Metabolite profiling of Mexican lime (Citrus aurantifolia) leaves during the progression of witches’ broom disease

Mollayi, Saeed; Zadali, Reza; Farzaneh, Mohsen; Ghassempour, Alireza

doi:10.1016/j.phytol.2015.07.010

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…Then, the DEMs in 5 PFI-75 vs. PFI, 6 PTI-75 vs. PTI, and 7 PTFI-75 vs. PTFI were compared. Similarly, the same DEMs in these three comparisons were generated 8 . Finally, 9 PaWB related to miRNAs were identified from the DEMs that intersected in 4 and 8 ( Figure 1).…”

Section: Identification Of Mirnas Related To Pawbmentioning

confidence: 99%

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Comparative Analysis of MicroRNA Expression in Three Paulownia Species with Phytoplasma Infection

Cao

Zhai

Zhang

et al. 2018

Forests

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Paulownia witches' broom (PaWB), caused by phytoplasma, is an important disease of Paulownia. To further identify the key miRNAs associated with the formation of PaWB symptoms, miRNA and degradome sequencing were performed to explore important miRNAs-target regulation in healthy and diseased Paulownia tomentosa, Paulownia fortunei, and P. tomentosa × P. fortunei seedlings, and the corresponding diseased seedlings treated with 75 mg L −1 dimethyl sulfate. A total of 212, 111, and 197 differentially expressed miRNAs (DEMs) were obtained in P. tomentosa, P. fortunei, and P. tomentosa × P. fortunei, respectively. Degradome sequencing detected 559, 251, and 568 target genes of the DEMs in P. tomentosa, P. fortunei, and P. tomentosa × P. fortunei, respectively. The expression patterns of selected miRNAs and the target genes were verified be qRT-PCR. Through analysis of the expression level of the DEMs in this study, combined with the results in our previous studies, as well as with those reported in other phytoplasma-infected plants, we concluded that miR156 is an important miRNA related to witches' broom. According to the functions of the target genes of DEMs, we constructed a co-regulatory network of the DEMs-target genes interaction. These results will help to advance the understanding of the mechanism of PaWB.

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Section: Identification Of Mirnas Related To Pawbmentioning

confidence: 99%

“…Therefore, researchers tend to focus on the molecular mechanisms in their host plants [4][5][6]. Accumulating evidence has revealed the gene, protein, and metabolite changes in the host plants by differential methods [7][8][9], and miRNA sequencing is one of the methods used to research the plant-phytoplasma interaction [7].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of MicroRNA Expression in Three Paulownia Species with Phytoplasma Infection

Cao

Zhai

Zhang

et al. 2018

Forests

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“…P. aurantifolia" infecting acid lime has indicated production of several metabolites significantly altered during infection. In Iran, infections are associated with catechin and epicatechin production in leaves [47,48]. Amino and organic acid concentrations (such as proline, arginine, glutamate, citrate and salicylate) are also significantly increased immediately after inoculation [47].…”

Section: Novel Asymptomatic Infection Biology: Results and Discussionmentioning

confidence: 99%

Asymptomatic Phytoplasma Reveal a Novel and Troublesome Infection

Donkersley¹,

Silva²,

Alves³

et al. 2020

Plant Diseases - Current Threats and Management Trends

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Asymptomatic infections are by their nature challenging to study and even more difficult to monitor across broad geographical ranges, particularly as methods are reliant on expensive molecular techniques. The plant pathogen that causes Witches' Broom disease of lime (Candidatus Phytoplasma aurantifolia) is a major limiting factor in lime production across the Middle East and was recently detected in Brazil, but without the typical symptoms from the Middle East. Here, we discuss the difficulty of monitoring asymptomatic infections and highlight the threat posed by highlight future outbreaks. Asymptomatic infections have important implications for understanding the evolution of pathogens within perennial hosts. We use three model systems of asymptomatic infections: (i) a Phytoplasma and (ii) a bacterial infection of lime (Citrus aurantifolia) and (iii) an "out-group" Phytoplasma of Cassava (Manihot esculenta) to demonstrate consistency across divergent hosts. We found that although all plants in the study were intentionally infected, assays typically did not confirm this diagnosis. Emergent technologies monitoring gene expression could be used to both study novel biology associated with asymptomatic infections and develop monitoring technologies. We highlight the difficulty of monitoring asymptomatic infections in possible future outbreaks and have important implications for understanding the evolution of pathogens within perennial hosts.

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“…Research on 40 metabolites of different categories including amino acids, fatty acids, organic acids, sugars and sugar alcohols has indicated that some compounds such as citrate, salicylate, arginine, glutamate, proline, inositol, fructose and some of the intermediates of TCA cycle displayed a significant increase 30 days after inoculation with Ca. P. aurantifolia (Mollayi et al 2015). In another study, 21 metabolites were analyzed in Witches' Broom Disease of Lime (WBDL)symptomatic leaves using GC-MS instrument.…”

Section: Introductionmentioning

confidence: 99%

Treatment of Lime Witches’ Broom Phytoplasma-Infected Mexican Lime with a Resistance Inducer and Study of its Effect on Systemic Resistance

Rastegar

Zamharir

Cai

et al. 2020

J Plant Growth Regul

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Destruction caused by plant diseases is terrible. Mexican lime witches' broom (MLWB) is a phytoplasma disease, which has caused considerable damage in Mexican lime tree in southern parts of Iran. In order to protect the plant against this pathogen, it is required to apply an appropriate treatment to induce resistance. Moreover, for better comprehension of disease mechanism, the analysis of phytohormones as one of the most critical basic components in plant cells, probably involved in resistance mechanisms, is of great importance. In this work, Mexican lime trees infected with lime witches'-broom phytoplasma (LWBP) were treated with Previcur Energy Tm (31% fosetyl-Alluminum plus 53% propamocarb) 4/ 000 as the resistance inducer against phytoplasma. In the next step, phytohormone levels in healthy Mexican lime were compared with those of infected and treated lime trees. For analysis of phytohormones, one-step dispersive solid phase extraction (DSPE) combined with liquid chromatography-tandem mass spectrometry-multiple reaction monitoring (LC-MS/MS-MRM) mode was used. A total of 54 phytohormones including cytokinins, auxins, jasmonates, gibberellins, salicylic acid and abscisic acid groups were simultaneously analyzed for treated, infected, and healthy plants. Totally, 18 phytohormones from plant leave extract were detected, among which levels of 11 phytohormones including SA, JA, JA-Phe, JA-Ile, JA-Leu, OPDA, CZ, iP7G, cZ9G, TZr and 2MeStZR were significantly changed in both infected and treated plants. Result of the present study indicated that the applied treatment was able to reduce the severity score in plant. On the other hand, phytoplasma (as a biotic stress) and treatment with Previcur Energy Tm (as a resistance inducer) could affect fundamental processes in plant via regulation of phytohormone levels.

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Metabolite profiling of Mexican lime (Citrus aurantifolia) leaves during the progression of witches’ broom disease

Cited by 9 publications

References 36 publications

Comparative Analysis of MicroRNA Expression in Three Paulownia Species with Phytoplasma Infection

Comparative Analysis of MicroRNA Expression in Three Paulownia Species with Phytoplasma Infection

Asymptomatic Phytoplasma Reveal a Novel and Troublesome Infection

Treatment of Lime Witches’ Broom Phytoplasma-Infected Mexican Lime with a Resistance Inducer and Study of its Effect on Systemic Resistance

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