Metabolite profiling of Ipomoea aquatica at different growth stages in correlation to the antioxidant and α-glucosidase inhibitory activities elucidated by 1H NMR-based metabolomics

Lawal, Umar; Mediani, Ahmed; Maulidiani, M.; Shaari, Khozirah; Ismail, Intan Safinar; Khatib, Alfi; Abas, Faridah

doi:10.1016/j.scienta.2015.06.036

Cited by 21 publications

(21 citation statements)

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“…Thus, the harvesting time should be optimized to preserve the plant’s health benefits and therapeutic properties of the final product. Determination of the appropriate growth stage for harvesting has provided valuable information on a plant’s nutritional and bioactivity properties [27, 29, 30]. It is interesting to note that C. caudatus extracted in EtOH:water (80:20) and harvested at the 10th week possess remarkable phenolic content and α-glucosidase inhibitory activity.…”

Section: Resultsmentioning

confidence: 99%

“…These changes may be caused by various factors, such as developmental stages [33, 34] and environmental conditions [32]. The study on Ipomoea aquatica by Lawal et al [27] revealed that the phenolic content of a plant increases as the plant grows older. Similar results were reported for the leaves of Moringa oleifera [70], in that a higher total phenolic content and antioxidant capacity were detected in the mature leaves compared to the young leaves.…”

Section: Resultsmentioning

confidence: 99%

“…The quality of plant material produced from herb and medicinal plant plantations is greatly influenced by a number of factors, including the environment, cultivation conditions, and management practises, such as temperature, irradiance, fertilizer supply, irrigation and harvesting time [27, 28]. The cultivated plant material should be harvested at the optimum growth stage when the parameters associated with the raw material quality are at their peak [27, 29–32]. This is because different metabolites are biosynthesized and may be present at different stages of plant growth.…”

Section: Introductionmentioning

confidence: 99%

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Variation in the metabolites and α-glucosidase inhibitory activity of Cosmos caudatus at different growth stages

Wan-Nadilah

Akhtar

Shaari

et al. 2019

BMC Complement Altern Med

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Background Cosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at different growth stages (6, 8. 10, 12 and 14 weeks) were determined in an effort to ascertain the best time to harvest the plant for maximum medicinal quality with respect to its glucose-lowering effects. Methods The aqueous ethanolic leaf extracts of C. caudatus were characterized by NMR and LC-MS/MS. The total phenolic content and α-glucosidase inhibitory activity were evaluated by the Folin-Ciocalteu method and α-glucosidase inhibitory assay, respectively. The statistical significance of the results was evaluated using one-way ANOVA with Duncan’s post hoc test, and correlation among the different activities was performed by Pearson’s correlation test. NMR spectroscopy along with multivariate data analysis was used to identify the metabolites correlated with total phenolic content and α-glucosidase inhibitory activity of the C. caudatus leaf extracts. Results It was found that the α-glucosidase inhibitory activity and total phenolic content of the optimized ethanol:water (80:20) leaf extract of the plant increased significantly as the plant matured, reaching a maximum at the 10th week. The IC 50 value for α-glucosidase inhibitory activity (39.18 μg mL − 1 ) at the 10th week showed greater potency than the positive standard, quercetin (110.50 μg mL − 1 ). Through an 1 H NMR-based metabolomics approach, the 10-week-old samples were shown to be correlated with a high total phenolic content and α-glucosidase inhibitory activity. From the partial least squares biplot, rutin and flavonoid glycosides, consisting of quercetin 3- O -arabinofuranoside, quercetin 3- O -rhamnoside, quercetin 3- O -glucoside, and quercetin 3- O -xyloside, were identified as the major bioactive metabolites. The metabolites were identified by NMR spectroscopy ( J -resolve, HSQC and HMBC experiments) and further supported by dereplication via LC-MS/MS. Conclusion For high phytomedicinal quality, the 10th week is recommended as the best time to harvest C. caudatus leaves with respect to its glucose lowering potential. Electronic supplementary material The online version of this article (10.1186/s12906-019-2655-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Variation in the metabolites and α-glucosidase inhibitory activity of Cosmos caudatus at different growth stages

Wan-Nadilah

Akhtar

Shaari

et al. 2019

BMC Complement Altern Med

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“…Lawal et al . 11 stated that the variability in the metabolite contents could be influenced by diverse factors, including plant harvest age or stage. Yusof et al .…”

Section: Introductionmentioning

confidence: 99%

“…The metabolite profile at various time points can be measured using several available state-of-the-art analytical tools, all with high reproducibility and sensitivity. Nuclear magnetic resonance (NMR) spectroscopy, in combination with chemometrics, is a proven technique used in metabolite profiling of various plant species 9,11,13,15 . NMR allows simultaneous detection of diverse groups of secondary metabolites and the metabolite quantification can be performed without the need for any calibration curves 15,16 .…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling of Andrographis paniculata (Burm. f.) Nees. young and mature leaves at different harvest ages using 1H NMR-based metabolomics approach

Tajidin

Shaari

Maulidiani

et al. 2019

Sci Rep

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Andrographis paniculata (Burm. F.) Nees. is considered as the herb of the future due to its precious chemical compounds, andrographolide (ANDRO), neoandrographolide (NAG) and 14-deoxyandrographolide (DAG). This study aims to profile the metabolites in young and mature leaf at six different harvest ages using 1HNMR-based metabolomics combined with multivariate data analysis. Principal component analysis (PCA) indicated noticeable and clear discrimination between young and mature leaves. A comparison of the leaves stage indicated that young leaves were separated from mature leaves due to its larger quantity of ANDRO, NAG, DAG, glucose and sucrose. These similar metabolites are also responsible for the PCA separation into five clusters representing the harvest age at 14, 16, 18, 20, 22 weeks of leaves extract. Loading plots revealed that most of the ANDRO and NAG signals were present when the plant reached at the pre-flowering stage or 18 weeks after sowing (WAS). As a conclusion, A. paniculata young leaves at pre-flowering harvest age were found to be richer in ANDRO, NAG and DAG compared to mature leaves while glucose and choline increased with harvest age. Therefore, young leaves of A. paniculata should be harvested at 18 WAS in order to produce superior quality plant extracts for further applications by the herbal, nutraceutical and pharmaceutical industries.

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Metabolomics based biomarker identification of anti-diabetes and anti-obesity properties of Malaysian herbs

et al. 2022

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Metabolite profiling of Ipomoea aquatica at different growth stages in correlation to the antioxidant and α-glucosidase inhibitory activities elucidated by 1H NMR-based metabolomics

Cited by 21 publications

References 38 publications

Variation in the metabolites and α-glucosidase inhibitory activity of Cosmos caudatus at different growth stages

Variation in the metabolites and α-glucosidase inhibitory activity of Cosmos caudatus at different growth stages

Metabolite profiling of Andrographis paniculata (Burm. f.) Nees. young and mature leaves at different harvest ages using 1H NMR-based metabolomics approach

Metabolomics based biomarker identification of anti-diabetes and anti-obesity properties of Malaysian herbs

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