Metabolite profiling, hypolipidemic, and anti-atherosclerosis activity of mixed vegetable fermentation extract

Rachmawati, Ermin; Suharti, Suharti; Sargowo, Djanggan; Kinasih, Larasati Sekar; Octaviano, Yudi Her; Mutiah, Roihatul; Ismail, Mahrus; Nasih, Ahmad Munjin

doi:10.1016/j.jsps.2023.03.004

Cited by 2 publications

(7 citation statements)

References 65 publications

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“…ROS molecules induce the expression of proinflammatory proteins by activating various transcription factors, including NF-κB [6]. Following HBO exposure, ROS molecules modify adaptive mechanisms that mitigate cell damage [3]. Hyperbaric oxygen exposure triggers signaling processes in cells involving proteins like heme oxygenase-1 (HO-1), Sirtuin-1 (SIRT1), and endothelial nitric oxide synthase (eNOS), which collectively exert a protective effect on endothelial dysfunction in animal models of endothelial dysfunction.…”

Section: Resultsmentioning

confidence: 99%

“…Antioxidants such as HO-1 and SIRT1 have been identified as cardioprotective proteins [7,8]. Hydrogen peroxide (H2O2) constitutes the majority of ROS generated during hyperbaric oxygen therapy (HBO) [3]. In the presence of ROS, cells do not necessarily undergo cell death.…”

Section: Resultsmentioning

confidence: 99%

“…Following hyperbaric oxygen (HBO) administration, reactive nitrogen species (RNS) and reactive oxygen species (ROS) levels are increased. Hydrogen peroxide (H2O2), as a reactive oxygen species (ROS), is subsequently converted into water (H2O) by enzymes such as catalase and glutathione peroxidase [3]. In addition to prevent oxygen toxicity, the inclusion of air breaks (intervals) of 5 minutes during HBO therapy creates a relatively hypoxic environment, leading to ischemic preconditioning.…”

Section: Hypoxiamentioning

confidence: 99%

“…Oxygen can reach bones and soft tissues that red blood cells cannot access, enhancing the function of white blood cells. It also promotes the formation of new capillaries (neovascularization) and peripheral blood vessels, thereby facilitating a rapid healing process [3]. HBO treatment increases oxygen transport to tissues through two processes.…”

Section: Hypoxiamentioning

confidence: 99%

“…These molecules act as signaling molecules in the transduction cascade for various transcription factors, growth factors, cytokines, and hormones. The beneficial or detrimental effects of these reactive species molecules depend on their concentration and intracellular localization [3]. Atherosclerosis, a prevalent cardiovascular disease in Indonesia, is often initiated by endothelial dysfunction resulting from factors such as hyperglycemia, hyperinsulinemia, hypertension, dyslipidemia, and obesity.…”

Section: Introductionmentioning

confidence: 99%

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2023

J. Med. Chem. Sci.

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Background:The utilization and advancement of hyperbaric science continue to evolve, encompassing the investigation of cardiovascular disease. Macrophage activation stimulates the expression of matrix metalloproteinases, specifically MMP-12, which play a crucial role in atherosclerosis by influencing vascular tissue. Methods: This study aimed to examine MMP-12 expression in cardiovascular disease induced by an atherogenic high-fat diet and explore the effects of hyperbaric oxygen therapy. Previous research has focused on the primary mechanism of hyperbaric oxygen therapy through the SIRT1 (Sirtuin) pathway, which addresses endothelial dysfunction in atherosclerosis treatment. However, the impact of HBO utilization on the treatment and prevention of cardiovascular disease remains unexplored. Results: Molecular analysis of MMP-12 was conducted using a rat model administered a high-fat diet, followed by HBO treatment. Endothelial dysfunction caused by the high-fat diet was identified as a marker of cardiovascular disease, assessed by endothelin-1 (ET-1) levels. The administration of hyperbaric oxygen (HBO) yielded significant improvements in atherosclerosis and heart disease. The results, with a pvalue of 0.05, were significant for both the treatment and prevention of atherosclerotic heart disease in the two groups used as models. Conclusion: Sprague Dawley rats subjected to hyperbaric oxygen therapy at a pressure of 2.4 ATA, three sessions of 30 minutes each, for ten consecutive days, demonstrated potential for application in cardiovascular disease prevention and treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Hypoxiamentioning

confidence: 99%

Section: Hypoxiamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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2023

J. Med. Chem. Sci.

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Vegetables Spices Fermentation Extract Inhibit NAFLD Development by Attenuation of Steatosis through Suppression of C/EBPα and TNFα mRNA Expression

Rachmawati,

Machdaniar,

Kinasih

et al. 2024

Indones Biomed J

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BACKGROUND: Non-alcoholic fatty liver disease (NAFLD), characterized by inflammation and steatosis, is one of the metabolic problems that trigger serious complications. Statin is usually used for NAFLD therapy, however, statin intolerance and resistance reduce the effectiveness of NAFLD treatment. Therefore, this study was conducted to develop the phytopharmaca against NAFLD by elucidating the anti-inflammatory and steatosis suppression of vegetable spices fermentation extract (VSFE) in rabbits induced with high-fat diet (HFD).METHODS: Twenty New Zealand White rabbits were determined into 4 groups; which were groups receiving standard diet (SD), HFD, HFD and 100 mg/kgBW VSFE, as well as HFD and 200 mg/kgBW VSFE. mRNA expressions of tumor necrosis factor alpha (TNFα) and CCAAT/enhancer-binding protein alpha (C/EBPα) were evaluated. The TNFα concentration was quantified after protein and RNA isolation. Inflammation and steatosis were observed in liver hematoxylin-eosin-stained preparate using microscope.RESULTS: Macrophage cells and hepatic steatosis showed significant decrease in a drug dependent manner. An effective dose of 200 mg/kgBW decreased C/EBPα and TNFα expression to 0.45±0.32 and 0.72±0.29 (p=0.013 and p=0.002, compared to the HFD group), respectively. TNFα inflammatory cytokine concentrations also responded to the administration of 100 mg/kgBW and 200 mg/kgBW doses, with the value of 31.72±10.40 mg/dL and 48.35±7.15 mg/dL (p=0.009 and p=0.002, compared to the HFD group), respectively.CONCLUSION: VSFE might prevent NAFLD by inhibit steatosis and inflammation through suppression of C/EBPα expression and TNFα expression.KEYWORDS: C/EBPα, TNFα, fermentation, steatosis, NAFLD

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Metabolite profiling, hypolipidemic, and anti-atherosclerosis activity of mixed vegetable fermentation extract

Cited by 2 publications

References 65 publications

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Untitled

Vegetables Spices Fermentation Extract Inhibit NAFLD Development by Attenuation of Steatosis through Suppression of C/EBPα and TNFα mRNA Expression

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