Metabolite Profiling Based on Lipophilic Compounds for Quality Assessment of Perilla (Perilla frutescens) Cultivars

Kim, Jae Kwang; Park, Soo-Yun; Na, Jong-Kuk; Seong, Eun Soo; Yu, Chang Yeon

doi:10.1021/jf204977x

Cited by 37 publications

(19 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies on the chemistry and biological activities of Perilla frutescens and its varieties, including plant parts described as leaves (Kaufmann et al, ; Meng, Lozano, Gaydou, & Li, ; Natsume, Muto, Fukuda, Tokunaga, & Osakabe, ; Zhu et al, ), seeds (Kim, Park, Na, Seong, & Yu, ; Lee et al, ; Peng, Ye, & Kong, ), and fruits (Gu, Wu, & Wang, ; Hong, Park, & Kim, ; Zhao, Qin, Wang, Chu, & Guo, ), have been reported. However, potential confusion over the plant part investigated may occur, as the nutlets (single‐seeded fruits) may be mistaken for seeds, thus raising the possibility that knowledge of the chemistry and biological activities of this species may not always be associated with the correct plant part, with implications for quality, safety, and efficacy, when plant material is traded and used for medicinal and food applications.…”

Section: Discussionmentioning

confidence: 99%

A chemically characterized ethanolic extract of Thai Perilla frutescens (L.) Britton fruits (nutlets) reduces oxidative stress and lipid peroxidation in human hepatoma (HuH7) cells

Paradee

Howes

Utama‐ang

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

A chemically characterized ethanolic extract of Thai Perilla frutescens (L.) Britton fruits (nutlets) reduces oxidative stress and lipid peroxidation in human hepatoma (HuH7) cells Perilla frutescens is cultivated in East Asian countries including Thailand, and the nutlets (single-seeded fruits) are used as traditional and medicinal food. Perilla nutlets extracted by ethyl acetate (EA), 80% ethanol (Eth), and hot water (HW) sequentially were chemically characterized using high-resolution accurate liquid chromatography-mass spectrometry with the main compounds detected assigned as rosmarinic acid and derivatives of the flavones apigenin and luteolin, with the more diverse chemical composition observed with the Eth extract. All extracts showed dose-dependent free-radical scavenging activity, with the Eth extract the most potent (IC 50 = 3.43 mg/ml for ABTS • scavenging and 0.27 mg/ml for DPPH • scavenging). The Eth extract also inhibited AAPH-induced hemolysis (IC 50 = 0.07 mg/ml) more potently than did the HW (IC 50 = 0.38 mg/ml) and EA extracts (IC 50 = 1.63 mg/ml). An MTT test revealed all the extracts were noncytotoxic at concentrations up to 200 μg/ml. Only the Eth and EA extracts showed protective effects against the generation of reactive oxygen species and lipid peroxidation in FeCl 3 -induced HuH7 cells in a dose-dependent manner. Our findings suggest the Eth extract of Thai perilla nutlets, containing rosmarinic acid and flavones and their derivatives, may have potential to provide protection against oxidative stress in hepatic disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

A chemically characterized ethanolic extract of Thai Perilla frutescens (L.) Britton fruits (nutlets) reduces oxidative stress and lipid peroxidation in human hepatoma (HuH7) cells

Paradee

Howes

Utama‐ang

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…For extraction of lipophilic metabolites, the powdered fruit samples (50 mg) were mixed with 3 ml of ethanol containing 0.1% ascorbic acid (w/v) as previously described ( Kim J.K. et al, 2012 ). 5α-cholestane (50 μl, 10 μg/ml) was used an as IS, mixed by vortexing for 20 s, and placed in a water bath at 85°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

An Integrated Biochemical, Proteomics, and Metabolomics Approach for Supporting Medicinal Value of Panax ginseng Fruits

et al. 2016

Self Cite

View full text Add to dashboard Cite

Panax ginseng roots are well known for their medicinal properties and have been used in Korean and Chinese traditional medicines for 1000s of years. However, the medicinal value of P. ginseng fruits remain poorly characterized. In this study, we used an integrated biochemical, proteomics, and metabolomics approach to look into the medicinal properties of ginseng fruits. DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] assays showed higher antioxidant activities in ginseng fruits than leaves or roots. Two-dimensional gel electrophoresis (2-DE) profiling of ginseng fruit proteins (cv. Cheongsun) showed more than 400 spots wherein a total of 81 protein spots were identified by mass spectrometry using NCBInr, UniRef, and an in-house developed RNAseq (59,251 protein sequences)-based databases. Gene ontology analysis showed that most of the identified proteins were related to the hydrolase (18%), oxidoreductase (16%), and ATP binding (15%) activities. Further, a comparative proteome analysis of four cultivars of ginseng fruits (cvs. Yunpoong, Gumpoong, Chunpoong, and Cheongsun) led to the identification of 22 differentially modulated protein spots. Using gas chromatography-time of flight mass spectrometry (GC-TOF MS), 66 metabolites including amino acids, sugars, organic acids, phenolic acids, phytosterols, tocopherols, and policosanols were identified and quantified. Some of these are well known medicinal compounds and were not previously identified in ginseng. Interestingly, the concentration of almost all metabolites was higher in the Chunpoong and Gumpoong cultivars. Parallel comparison of the four cultivars also revealed higher amounts of the medicinal metabolites in Chunpoong and Gumpoong cultivars. Taken together, our results demonstrate that ginseng fruits are a rich source of medicinal compounds with potential beneficial health effects.

show abstract

“…This allows the analysis of multiple variables at the same time and draws a smaller number of variables, which allow for the visualization of data [33]. The two most common multivariate analyses are the principal component analysis (PCA), which is an unsupervised test, and the orthogonal partial least square discriminate analysis (OPLS-DA), which is a supervised test employed to build a predictive model [23].…”

Section: Introductionmentioning

confidence: 99%

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Montani

Cordeiro

Regiani

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy. Method Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built. Results S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the nonpregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation. Conclusion We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.

show abstract

Metabolite Profiling Based on Lipophilic Compounds for Quality Assessment of Perilla (Perilla frutescens) Cultivars

Cited by 37 publications

References 20 publications

A chemically characterized ethanolic extract of Thai Perilla frutescens (L.) Britton fruits (nutlets) reduces oxidative stress and lipid peroxidation in human hepatoma (HuH7) cells

A chemically characterized ethanolic extract of Thai Perilla frutescens (L.) Britton fruits (nutlets) reduces oxidative stress and lipid peroxidation in human hepatoma (HuH7) cells

An Integrated Biochemical, Proteomics, and Metabolomics Approach for Supporting Medicinal Value of Panax ginseng Fruits

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Contact Info

Product

Resources

About