Metabolite Profiling Approach Reveals the Interface of Primary and Secondary Metabolism in Colored Cauliflowers (Brassica oleracea L. ssp. <i>botrytis</i>)

Park, Soo-Yun; Lim, Sung-Hun; Ha, Sun-Hwa; Yeo, Yunsoo; Park, Woo-Tae; Kwon, Donghyok; Park, Sang Un; Kim, Jae Kwang

doi:10.1021/jf401330e

Cited by 55 publications

(42 citation statements)

References 25 publications

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“…As shown in Table 2, all samples contained six phenolic acids, including p-hydroxybenzoic, vanillic, syringic, p-coumaric, ferulic, and sinapic acid, which were previously identified as phenolics by our group using GC-TOFMS (8,20). However, the levels of these compounds in sweet potatoes with different tuber colors have not been previously evaluated.…”

Section: Resultsmentioning

confidence: 99%

“…ChromaTOF software was used to assist with peak location and for automated deconvolution of reference mass spectra. The peaks were identified by comparison with reference compounds and an in-house library (8). In addition, several metabolites were identified by directly comparing their sample mass chromatograms with those of commercially available standard compounds, which were obtained by a similar MO/TMS derivatization and GC-TOFMS analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Extraction and analysis of anthocyanins Anthocyanin extraction was performed according to method of Park et al (8). Anthocyanin was separated in a C 18 column (250×4.6 mm, 5 µm, Inertsil ODS-3; GL Sciences) by HPLC as described above.…”

Section: Methodsmentioning

confidence: 99%

“…Metabolite profiling Polar metabolite extraction was performed as described previously (8). Ribitol was used as an IS.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, hydrophilic primary metabolites from sweet potatoes were profiled using gas chromatography-time-of-flight mass spectrometry (GC-TOFMS) to determine phenotypic variations and relationships among metabolite contents. A GC-TOFMS-based metabolic profiling analysis facilitates rapid and highly sensitive detection of plant metabolites from the central pathways of primary metabolism (7,8). Carotenoids, flavonoids, anthocyanins, and phenolic acids were quantified as bioactive secondary metabolites to evaluate the quality of three sweet potato varieties.…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers

Park

Lee

Yang

et al. 2016

Food Sci Biotechnol

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We determined the phytochemical diversity, including carotenoids, flavonoids, anthocyanins, and phenolic acids, in sweet potatoes ( L.) with distinctive flesh colors (white, orange, and purple) and identified hydrophilic primary metabolites. Carotenoid content was considerably higher in orange-fleshed sweet potatoes, wherein β-carotene was the most plentiful, and anthocyanins were detected only in purple-fleshed sweet potatoes. The levels of phenolic acids and flavonoids were relatively higher in purple-fleshed sweet potatoes than those in the other two varieties. Forty-one primary and 18 secondary metabolite profiles were subjected to multivariate statistical analyses, which fully distinguished among the varieties and separated orange- and purple-fleshed sweet potatoes from white-fleshed sweet potatoes based on the high levels of sugars, sugar alcohols, and secondary metabolites. This is the first study to determine comprehensive metabolic differences among different color-fleshed sweet potatoes and provides useful information for genetic manipulation of sweet potatoes to influence primary and secondary metabolism.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Metabolite profiling Polar metabolite extraction was performed as described previously (8). Ribitol was used as an IS.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers

Park

Lee

Yang

et al. 2016

Food Sci Biotechnol

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Broccoli and Cauliflower

Bhattacharjee

2018

Handbook of Vegetables and Vegetable Processing

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Landscape‐dependent effects of varietal mixtures on insect pest control and implications for farmer profits

Snyder

Gómez

Mudrak

et al. 2021

Ecological Applications

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Intraspecific plant diversity can significantly impact insect herbivore populations in natural systems. Yet, its role as an insect pest control strategy in agriculture has received less attention, and little is known about which crop traits are important to herbivores in different landscape contexts. Moreover, empirical economic analyses on the cost-effectiveness of varietal mixtures is lacking. We used varietal mixtures of Brassica oleracea crops on working farms to examine how two metrics of intraspecific crop diversity-varietal richness and number of plant colors (color richness)-affect crop damage and the incidence and abundance of two insect pest species, Pieris rapae and Phyllotreta spp. We evaluated the context-dependency of varietal mixtures by sampling early and late season plantings of B. oleracea crops in farms across a gradient of landscape composition. We developed crop budgets and used a net present value analysis to assess the impact of varietal mixtures on input and labor costs, crop revenues, and profit. We found context-dependent effects of varietal mixtures on both pests. In early season plantings, color richness did not affect Phyllotreta spp. populations. However, increasing varietal richness reduced Phyllotreta spp. incidence in simple landscapes dominated by cropland, but this trend was reversed in complex landscapes dominated by natural habitats. In late season plantings, color richness reduced the incidence and abundance of P. rapae larvae, but only in complex landscapes where their populations were highest. Varietal richness had the same effect on P. rapae larvae as color richness. Unexpectedly, we consistently found lower pest pressure and reduced crop damage in simple landscapes. Although varietal mixtures did not affect crop damage, increasing color richness corresponded with increased profits, due to increased revenue and a marginal reduction in labor and input costs. We demonstrate varietal mixtures can significantly impact pest populations, and this effect can be mediated by intraspecific variation in crop color. However, the strength and direction of these effects vary by season, landscape composition, and pest species. The association between varietal color richness and profitability indicates farmers could design mixtures to enhance economic returns. We recommend additional research on the benefits of intraspecific trait variation for farmers.

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Metabolite Profiling Approach Reveals the Interface of Primary and Secondary Metabolism in Colored Cauliflowers (Brassica oleracea L. ssp. botrytis)

Cited by 55 publications

References 25 publications

Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers

Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers

Broccoli and Cauliflower

Landscape‐dependent effects of varietal mixtures on insect pest control and implications for farmer profits

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