Metabolite levels in human skeletal muscle and adipose  tissue studied with microdialysis at low perfusion flow

Rosdahl, Hans; Hamrin, Kerstin; Ungerstedt, Urban; Henriksson, Jan

doi:10.1152/ajpendo.1998.274.5.e936

Cited by 87 publications

(134 citation statements)

References 17 publications

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“…While the concentrations of glucose and lactate in the dialysate from skeletal muscle are close to the expected values, the glycerol values obtained in muscle are puzzling. We have repeatedly found very high values during the first hour following catheter insertion, but thereafter glycerol values are lower than in venous blood (Rosdahl et al 1998a). However, a similar finding was not obtained in a study by Hagström-Toft et al (1997).…”

Section: Interstitial Concentrations May Be Measured With Microdialyssupporting

confidence: 55%

“…This explanation is in agreement with the results of studies of other investigators (Maggs et al 1995;Müller et al 1996), and most studies report that glucose concentrations in plasma equal those in the interstitial space of adipose tissue (Lönnroth et al 1987;Bolinder et al 1989). The result is further supported by the finding that urea, which is not taken up by skeletal muscle, is not lower in skeletal muscle dialysates than in plasma (Rosdahl et al 1998a), and is not different at low flow rates in skeletal muscle and adipose tissue dialysates. Changes in the interstitial glucose concentration during insulin stimulation should reflect the insulin sensitivity of the tissue (Rosdahl et al 1998b).…”

Section: Interstitial Concentrations May Be Measured With Microdialyssupporting

confidence: 53%

“…Results were collected from microdialysis catheters which were inserted in the m. quadriceps femoris of healthy subjects and perfused at a constant rate for 8 h (Rosdahl et al 1998a). Briefly, the results indicate that glucose, lactate and glycerol levels stabilized 90 min after catheter insertion, whereas urea levels stabilized faster.…”

Section: Stability Over Timementioning

confidence: 99%

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Microdialysis of skeletal muscle at rest

Henriksson

1999

Proc. Nutr. Soc.

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Techniques in human skeletal muscle research are by necessity predominantly 'descriptive'.Microdialysis has raised high expectations that it could meet the demand for a method that allows 'mechanistic' investigations to be performed in human skeletal muscle. In the present review, some views are given on how well the initial expectations on the use of the microdialysis technique in skeletal muscle have been fulfilled, and the areas in which additional work is needed in order to validate microdialysis as an important metabolic technique in this tissue. The microdialysis catheter has been equated to an artificial blood vessel, which is introduced into the tissue. By means of this 'vessel' the concentrations of compounds in the interstitial space can be monitored. The concentration of substances in the collected samples is dependent on the rate of perfusate flow. When perfusate flow is slow enough to allow complete equilibration between interstitial and perfusate fluids, the concentration in the perfusate is maximal and identical to the interstitial concentration. Microdialysis data may be influenced by changes in blood flow, especially in instances where the tissue diffusivity limits the recovery in vivo, i.e. when recovery in vitro is 100 %, whereas the recovery in vivo is less than 100 %. Microdialysis data indicate that a significant arterial-interstitial glucose concentration gradient exists in skeletal muscle but not in adipose tissue at rest. While the concentrations of glucose and lactate in the dialysate from skeletal muscle are close to the expected values, the glycerol values obtained for muscle are still puzzling. Ethanol added to the perfusate will be cleared by the tissue at a rate that is determined by the nutritive blood flow (the microdialysis ethanol technique). It is concluded that microdialysis of skeletal muscle has become an important technique for mechanistic studies in human metabolism and nutrition.

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Section: Interstitial Concentrations May Be Measured With Microdialyssupporting

confidence: 55%

Section: Interstitial Concentrations May Be Measured With Microdialyssupporting

confidence: 53%

Section: Stability Over Timementioning

confidence: 99%

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Microdialysis of skeletal muscle at rest

Henriksson

1999

Proc. Nutr. Soc.

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“…Significant glycerol concentration differences (asterisk) were found from 60 to 160 min during surgery between the paraspinal muscle and the deltoid muscle (f) the MD membrane, tissue temperature, and so on. Previous studies suggested that with a longer MD membrane (3 cm) and a slower perfusion rate (0.33 ll/min), the recovery close to 100% [17]. In the present study, relative recovery was not evaluated.…”

Section: Microdialysismentioning

confidence: 68%

“…The technique has been showed to be valuable in many tissues of human being, including skeletal muscles [17,18], even in the clinical settings [12,14,16].…”

Section: Microdialysismentioning

confidence: 99%

Microdialysis of paraspinal muscle in healthy volunteers and patients underwent posterior lumbar fusion surgery

et al. 2009

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Paraspinal muscle damage is inevitable during conventional posterior lumbar fusion surgery. Minimal invasive surgery is postulated to result in less muscle damage and better outcome. The aim of this study was to monitor metabolic changes of the paraspinal muscle and to evaluate paraspinal muscle damage during surgery using microdialysis (MD). The basic interstitial metabolisms of the paraspinal muscle and the deltoid muscle were monitored using the MD technique in eight patients, who underwent posterior lumbar fusion surgery (six male and two female, median age 57.7 years, range 37-74) and eight healthy individuals for different positions (five male and three female, age 24.1 ± 0.8 years). Concentrations of glucose, glycerol, and lactate pyruvate ratio (L/P) in both tissues were compared. In the healthy group, the glucose and glycerol concentrations and L/P were unchanged in the paraspinal muscle when the body position changed from prone to supine. The glucose concentration and L/P were stable in the paraspinal muscle during the surgery. Glycerol concentrations increased significantly to 243.0 ± 144.1 lM in the paraspinal muscle and 118.9 ± 79.8 lM in the deltoid muscle in the surgery group. Mean glycerol concentration difference (GCD) between the paraspinal muscle and the deltoid tissue was 124.1 lM (P = 0.003, with 95% confidence interval 83.4-164.9 lM). The key metabolism of paraspinal muscle can be monitored by MD during the conventional posterior lumbar fusion surgery. The glycerol concentration in the paraspinal muscle is markedly increased compared with the deltoid muscle during the surgery. It is proposed that GCD can be used to evaluate surgery related paraspinal muscle damage. Changing body position did not affect the paraspinal muscle metabolism in the healthy subjects.

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Metabolism in the Contracting Skeletal Muscle

Henriksson¹

2000

Endurance in Sport

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Metabolite levels in human skeletal muscle and adipose tissue studied with microdialysis at low perfusion flow

Cited by 87 publications

References 17 publications

Microdialysis of skeletal muscle at rest

Microdialysis of skeletal muscle at rest

Microdialysis of paraspinal muscle in healthy volunteers and patients underwent posterior lumbar fusion surgery

Metabolism in the Contracting Skeletal Muscle

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