Metabolite Exchange between Mammalian Organs Quantified in Pigs

Jang, Cholsoon; Shen, Hui; Zhang, Xianfeng; Cowan, Alexis; Wang, Lin; Chen, Li; Morscher, Raphael J.; Reyes, Jorgé; Frezza, Christian; Hwang, Ho Young; Imai, Akito; Saito, Yoshiaki; Okamoto, Keitaro; Vaspoli, Christine; Kasprenski, Loewe; Zsido, Gerald A; Gorman, Joseph H.; Gorman, Robert C.; Rabinowitz, Joshua D.

doi:10.1016/j.cmet.2022.08.006

Cited by 4 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lysine has recently attracted attention for its ability to suppress saltsensitive hypertension 91 . Although lysine is one of the essential amino acids, we found it was released from the SD rat kidney after HS (Figure 7), consistent with observations by Jang et al 17 in pig study. This is likely explained by the degradation of protein either by glomerular epithelial or tubular lysosomes during renal passage [92][93][94] .…”

Section: Compensatory Mechanisms To Protect From Hypertension and Kid...supporting

confidence: 92%

“…Studies carried out in fasted swine found glutamine and proline from the arterial blood are largely disposed of by the kidneys and other amino acids such as serine, tyrosine and arginine generated and released from the kidneys for export to other tissues 17 . The current study carried out in unanesthetized non-fasted rats found glutamine was not taken up at LS state but a strong tendency for an increased uptake of glutamine (p=0.08) was observed during HS feeding.…”

Section: Compensatory Mechanisms To Protect From Hypertension and Kid...mentioning

confidence: 99%

“…With the emergence of large-scale mass spectrometry and analysis tools (aka metabolomics), it is currently possible to identify and prioritize several thousands of detected features providing a comprehensive analysis in a tissue specimen of the changes in metabolism that occur across various organs of the body. The potential power of 4 these methods was demonstrated recently in a study by Jang et al 17 who analyzed the arterial and venous blood of 11 organs in fasted pigs and mapped more than 700 cases of organspecific metabolite production or consumption. More relevant to the current study, Rinschen et al 18 recently carried out a metabolomic and proteomic analysis of the kidney glomeruli and cortical tissue to determine the effects of a high salt diet in a naturally occurring rat model of salt-sensitive hypertension, the Dahl SS rat.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolic responses of normal rat kidneys to a high salt intake

Shimada

Yang

Kurth

et al. 2023

Preprint

View full text Add to dashboard Cite

In the present study, novel methods were developed which allowed continuous (24/7) measurement of blood pressure (BP) and renal blood flow (RBF) in freely moving rats and the intermittent collection of arterial and renal venous blood to estimate kidney metabolic fluxes of O2 and metabolites. The study determined the effects of a high salt (HS) diet upon whole kidney O2 consumption and the metabolomic profiles of normal Sprague Dawley (SD) rats. A separate group of rats was studied to determine changes in the cortex (Cx) and outer medulla (OM) tissue metabolomic and mRNAseq profiles before and following the switch from a 0.4% to a 4.0% NaCl diet. Significant changes in the metabolomic and transcriptomic profiles occurred with feeding of the HS diet. A progressive increase of kidney O2 consumption was found despite a reduction in expression of most of the mRNA encoding enzymes of TCA cycle. Increased glycolysis was evident with the elevation of mRNA expression encoding key glycolytic enzymes and release of pyruvate and lactate from the kidney in the renal venous blood. Glycolytic production of NADH is used in either the production of lactate or oxidized via the malate aspartate shuttle. Aerobic glycolysis (e.g., Warburg-effect) may account for the needed increase in cellular energy. The study provides interesting and provocative new data of how normal kidneys respond to a HS diet and guides us toward deeper mechanistic studies examining the effects of a HS diet upon kidney metabolism.

show abstract

Section: Compensatory Mechanisms To Protect From Hypertension and Kid...supporting

confidence: 92%

Section: Compensatory Mechanisms To Protect From Hypertension and Kid...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic responses of normal rat kidneys to a high salt intake

Shimada

Yang

Kurth

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…A previous study examining metabolites in the pig circulation showed similar metabolite levels in various arterial sites, whereas altered levels in veins draining an organ compared with the arterial circulation reflected organ-specific metabolism. 27 The ciliary artery feeds the choroid circulation, bringing metabolites and oxygen to photoreceptors in the outer retina, and vortex veins drain quadrants of the choroid. 28 We performed experiments comparing levels of metabolites involved in SAM metabolism in the arterial circulation entering the choroid with levels in the four vortex veins ( Figures 5A – 5D ).…”

Section: Resultsmentioning

confidence: 99%

Metabolic transcriptomics dictate responses of cone photoreceptors to retinitis pigmentosa

Lee,

Emery,

Vukmanic

et al. 2023

Cell Reports

View full text Add to dashboard Cite

“…It is known that different tissues have unique metabolite and lipid profiles. 152,153 For example, organ-specific lipidomics studies reveal sterols are more concentrated in the liver and kidney, ubiquinone is more abundant in muscle tissue, and 24S-hydroxycholesterol is elevated in the brain. 152,154 Lipid composition can also be altered by infection and inflammation.…”

Section: Adding Cholesterol To Cultures Either In the Form Of Ldl Ormentioning

confidence: 99%

Decoding the crosstalk between mevalonate metabolism and T cell function

Kennewick

Bensinger

2023

Immunological Reviews

View full text Add to dashboard Cite

SummaryThe mevalonate pathway is an essential metabolic pathway in T cells regulating development, proliferation, survival, differentiation, and effector functions. The mevalonate pathway is a complex, branched pathway composed of many enzymes that ultimately generate cholesterol and nonsterol isoprenoids. T cells must tightly control metabolic flux through the branches of the mevalonate pathway to ensure sufficient isoprenoids and cholesterol are available to meet cellular demands. Unbalanced metabolite flux through the sterol or the nonsterol isoprenoid branch is metabolically inefficient and can have deleterious consequences for T cell fate and function. Accordingly, there is tight regulatory control over metabolic flux through the branches of this essential lipid synthetic pathway. In this review we provide an overview of how the branches of the mevalonate pathway are regulated in T cells and discuss our current understanding of the relationship between mevalonate metabolism, cholesterol homeostasis and T cell function.

show abstract

Metabolite Exchange between Mammalian Organs Quantified in Pigs

Cited by 4 publications

References 0 publications

Metabolic responses of normal rat kidneys to a high salt intake

Metabolic responses of normal rat kidneys to a high salt intake

Metabolic transcriptomics dictate responses of cone photoreceptors to retinitis pigmentosa

Decoding the crosstalk between mevalonate metabolism and T cell function

Contact Info

Product

Resources

About