Metabolite and expression profiling of steroidal alkaloids in wild tissues compared to bulb derived in vitro cultures of Fritillaria roylei – High value critically endangered Himalayan medicinal herb

Kumar, Pankaj; Partap, Mahinder; Ashrita,; Rana, Divya; Kumar, Pawan; Warghat, Ashish R.

doi:10.1016/j.indcrop.2019.111945

Cited by 36 publications

(11 citation statements)

References 30 publications

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“…Total phenolic content was calculated using the method of Kumar et al (2020) . In 1.5 mL Folin-Ciocalteu Reagent (1 F.C.…”

Section: Methodsmentioning

confidence: 99%

“…In 1.5 mL Folin-Ciocalteu Reagent (1 F.C. : 9 Water), 300 μL of methanol extract was added and incubated at room temperature for 10 min in dark conditions ( Kumar et al, 2020 ). After that 1.2 mL of 2.5% Na 2 CO 3 solution was added and the mixture was kept idle for 1 h. Absorbance was taken at 765 nm using a Shimadzu 8000 UV-Vis spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

“…In 1.5 mL Folin-Ciocalteu Reagent (1 F.C. : 9 Water), 300 µL of methanol extract was added and incubated at room temperature for 10 min in dark conditions (Kumar et al, 2020).…”

Section: Total Phenolic Contentmentioning

confidence: 99%

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Agro-Climatic Suitability Evaluation for Saffron Production in Areas of Western Himalaya

et al. 2021

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Saffron (Crocus sativus L.) is an expensive spice crop cultivated successfully in Iran, Afghanistan, India, Greece, Morocco, Spain, and Italy. The present study was conducted during the periods 2018–2019 and 2019–2020 to evaluate the morphological, yield and quality parameters of saffron in six different regions of non-traditional areas of the western Himalayas. The two experimental factors were “year” and “location.” The experiment was conducted using a factorial randomized block design with three replications. Yield attributes, viz., number of flowers, fresh flower yield, fresh stigma yield and dry stigma yield were significantly higher in location L3 compared to other geographical locations. Dry stigma yield in location L3 was higher by 50.0, 41.2, 33.3, 14.3, and 9.1% compared to locations L6, L5, L1, L4, and L2, respectively. These were characterized by the appropriate climatic conditions, viz., high altitude, sandy-loam texture of the soil, optimum temperature, lesser relative humidity and total rainfall, demonstrating that it is possible to cultivate this spice even in non-traditional areas of the western Himalaya. Positive correlations were established for stigma yield with increased altitude and lesser rainfall. Secondary metabolites viz., crocin and picrocrocin increased significantly with the increase in altitude; however, a reverse trend was recorded for safranal content. Total phenolics and flavonoids were significantly higher in the geographical location of Kinnaur, H.P. (L1) and Bharmour, H.P. (L4). In conclusion, the assessment of different geographical locations and soil types is particularly necessary to encourage saffron production and its qualitative traits. Based on current findings, saffron can be grown successfully in some non-traditional locations of the western Himalayan regions.

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“…Total phenolic content was calculated using the method of Kumar et al (2020) . In 1.5 mL Folin-Ciocalteu Reagent (1 F.C.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Agro-Climatic Suitability Evaluation for Saffron Production in Areas of Western Himalaya

et al. 2021

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“…Fritillaria is the biopharmaceutical source of many drug compounds used in traditional Chinese medicine [ 4 ]. The bulbs are famed for important bioactive compounds such as glycosides, terpenoids, saponins, and steroidal alkaloids [ 1 , 3 , 5 , 6 , 7 ]. Nonetheless, the flower, leaf, root, and stem are also used in folk medicine for their therapeutic and curative properties [ 4 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The abundant pharmacological and phytochemical profiles in Fritillaria species have made them the objects of diverse studies, and a broad variety of new steroid alkaloids have been recently isolated [ 7 , 10 ]. Understanding biosynthetic pathways is necessary for the efficient utilization of key phytochemicals present in F. hupehensis [ 5 , 9 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing Provides Insights into Flavonoid Biosynthesis in Fritillaria hupehensis

Guo

Chen²,

Niu³

et al. 2021

Life

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One of the most commonly utilized medicinal plants in China is Fritillaria hupehensis (Hsiao et K.C. Hsia). However, due to a lack of genomic resources, little is known about the biosynthesis of relevant compounds, particularly the flavonoid biosynthesis pathway. A PacBio RS II sequencing generated a total of 342,044 reads from the bulb, leaf, root, and stem, of which 316,438 were full-length (FL) non-redundant reads with an average length of 1365 bp and a N50 of 1888 bp. There were also 38,607 long non-coding RNAs and 7914 simple sequence repeats detected. To improve our understanding of processes implicated in regulating secondary metabolite biosynthesis in F. hupehensis tissues, we evaluated potential metabolic pathways. Overall, this study provides a repertoire of FL transcripts in F. hupehensis for the first time, and it will be a valuable resource for marker-assisted breeding and research into bioactive compounds for medicinal and pharmacological applications.

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Integrated Approach for the Quality Assurance of Commercially Important Himalayan Medicinal Plants

Bora

Suresh

Kumari

et al. 2021

Sustainable Development and Biodiversity

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Metabolite and expression profiling of steroidal alkaloids in wild tissues compared to bulb derived in vitro cultures of Fritillaria roylei – High value critically endangered Himalayan medicinal herb

Cited by 36 publications

References 30 publications

Agro-Climatic Suitability Evaluation for Saffron Production in Areas of Western Himalaya

Agro-Climatic Suitability Evaluation for Saffron Production in Areas of Western Himalaya

Full-Length Transcriptome Sequencing Provides Insights into Flavonoid Biosynthesis in Fritillaria hupehensis

Integrated Approach for the Quality Assurance of Commercially Important Himalayan Medicinal Plants

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