Metabolismo de serina: caracterização de serina hidroximetiltransferase de <i>Trypanosoma cruzi</i>.

Abstract

Chagas' disease is a disorder caused by the protozoan parasite Trypanosoma cruzi, which affects about 10 million people, mainly in the Americas. T. cruzi uses amino acids as an important energy source and in several biological processes such as differentiation, resistance to stress conditions and in the host-cell invasion. Serine is involved in many biosynthetic pathways. One of the relevant functions of serine is the formation of C1 compounds for the biosynthesis of nucleotides. The use of serine for that purpose is initiated by Serine Hydroxymethyltransferase, whose activity was detected in T. cruzi but its role in the biology of parasite remains poorly explored. In this work we identified a putative gene encoding a SHMT with dual localization, cytoplasmic and mitochondrial. We generated a single knockout cell line by homologous recombination in which one allele of SHMT was replaced by the neomycin phosphotransferase gene. Knockout parasites showed no difference in epimastigote growth rate or in in vitro metacyclogenesis. However, knockout parasites showed a significant decrease in both, infection index and in the number of trypomastigotes released from CHO-K1cells infected with knockout metacyclic forms.