Metabolism responses in the intestine of Oreochromis mossambicus exposed to salinity, alkalinity and salt-alkalinity stress using LC-MS/MS-based metabolomics

Su, Huanhuan; Li, Yaya; Ma, Dongmei; Fan, Jiajia; Zhong, Zaixuan; Zhu, Hai‐Lei

doi:10.1016/j.cbd.2022.101044

Cited by 6 publications

(4 citation statements)

References 101 publications

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“…The increase in betaine in the mussel mantle indicated osmotic pressure from acute acidification indicating that it may help cope with external osmotic stress. Similar results have been reported in fish [65] and shrimp [66] under different salinity stresses. Asp is a proteinogenic amino acid and an important metabolite of energy metabolism pathways, such as the tricarboxylic acid cycle (TCA).…”

Section: Plos Onesupporting

confidence: 90%

Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification

Fan,

Wang,

Tang

et al. 2023

PLoS ONE

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Mytilus coruscus is an economically important marine bivalve mollusk found in the Yangtze River estuary, which experiences dramatic pH fluctuations due to seasonal freshwater input and suffer from shell fracture or injury in the natural environment. In this study, we used intact-shell and damaged-shell M. coruscus and performed metabolomic analysis, free amino acids analysis, calcium-positive staining, and intracellular calcium level tests in the mantle to investigate whether the mantle-specific metabolites can be induced by acute sea-water acidification and understand how the mantle responds to acute acidification during the shell repair process. We observed that both shell damage and acute acidification induced alterations in phospholipids, amino acids, nucleotides, organic acids, benzenoids, and their analogs and derivatives. Glycylproline, spicamycin, and 2-aminoheptanoic acid (2-AHA) are explicitly induced by shell damage. Betaine, aspartate, and oxidized glutathione are specifically induced by acute acidification. Our results show different metabolic patterns in the mussel mantle in response to different stressors, which can help elucidate the shell repair process under ocean acidification. furthermore, metabolic processes related to energy supply, cell function, signal transduction, and amino acid synthesis are disturbed by shell damage and/or acute acidification, indicating that both shell damage and acute acidification increased energy consumption, and disturb phospholipid synthesis, osmotic regulation, and redox balance. Free amino acid analysis and enzymatic activity assays partially confirmed our findings, highlighting the adaptation of M. coruscus to dramatic pH fluctuations in the Yangtze River estuary.

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Section: Plos Onesupporting

confidence: 90%

Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification

Fan,

Wang,

Tang

et al. 2023

PLoS ONE

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“…The regulatory-associated protein of MTOR complex 1 ( RPTOR ) is associated with the mammalian target of rapamycin (mTOR), which plays a key role in controlling and shaping the effector responses of innate immune cells (Weichhart et al, 2015). The activation of this pathway has been identified using transcriptomics in the euryhaline fish Oreochromis mossambicus following osmotic stress (Su et al, 2023). In conclusion, our findings highlight a comprehensive differential expression of immune system processes and stress-related pathways, emphasizing the intricate molecular responses of minnow to salinity stress in a polluted environment.…”

Section: Discussionmentioning

confidence: 99%

Unravelling the molecular mechanisms of fish salinity adaptation in the face of multiple stressors: A comparative multi-tissue transcriptomic study in the Llobregat River, Barcelona, Spain

Escobar-Sierra,

Cañedo-Argüelles,

Vinyoles

et al. 2023

Preprint

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Freshwater salinization poses a growing global environmental concern, introducing complex chemical cocktails and jeopardizing freshwater biodiversity, particularly fish populations. This research aimed to elucidate the molecular foundations of salinity adaptation in a non-native minnow species (Phoxinus septimaniaexP. dragarum) exposed to saline effluents from potash mines in the Llobregat River, Barcelona, Spain. Employing high-throughput mRNA sequencing and differential gene expression analyses, we examined brain, gills, and liver tissues collected from fish at two stations (upstream and downstream of saline effluent discharge). Salinization markedly influenced global gene expression profiles, with the brain exhibiting the most differentially expressed genes, emphasizing its unique sensitivity to salinity fluctuations. Pathway analyses revealed the expected enrichment of ion transport and osmoregulation pathways across all tissues. Furthermore, tissue-specific pathways associated with stress, reproduction, growth, immune responses, methylation, and neurological development were identified in the context of salinization. Rigorous validation of RNA-seq data through quantitative PCR (qPCR) underscored the robustness and consistency of our findings across platforms. This investigation unveils intricate molecular mechanisms steering salinity adaptation in non-native minnows confronting diverse environmental stressors. Advancing our comprehension of genomic responses to salinity changes, our study provides crucial insights into the adaptive strategies of aquatic organisms grappling with freshwater salinization. This comprehensive analysis sheds light on the underlying genetic and physiological mechanisms governing fish adaptation in salinity-stressed environments, offering essential knowledge for the conservation and management of freshwater ecosystems facing escalating salinization pressures.

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“…Currently, this approach is widely applied to unravel the intricate metabolic mechanisms through which aquatic organisms respond to fluctuations in external environmental factors [19,20]. Employing LC-MS/MS-based metabolomics, Su et al [21] explored the metabolic responses of Mozambique tilapia (Oreochromis mossambicus) under varying saline-alkaline concentrations. Their study elucidated its molecular regulatory mechanisms in response to different osmotic pressures.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Impact of Disrupting the Glutamine Metabolism Pathway on Ammonia Excretion in Crucian Carp (Carassius auratus) under Carbonate Alkaline Stress Using Metabolomics Techniques

Sun,

Geng,

Liu

et al. 2024

Antioxidants

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With the gradual decline in freshwater resources, the space available for freshwater aquaculture is diminishing and the need to maximize saline water for aquaculture is increasing. This study aimed to elucidate the impact mechanisms of the disruption of the glutamate pathway on serum metabolism and ammonia excretion in crucian carp (Carassius auratus) under carbonate alkaline stress. A freshwater control group (C group), a 20 mmol/L NaHCO3 stress group (L group), and a 40 mmol/L NaHCO3 stress group (H group) were established. After 30 days of exposure, methionine sulfoximine (MSO) was injected to block the glutamate pathway metabolism, and the groups post-blocking were labeled as MC, ML, and MH. Ultra-high-performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) metabolomics technique was employed to detect changes in the composition and content of crucian carp serum metabolites. Significant differential metabolites were identified, and related metabolic pathways were analyzed. The results revealed that, following the glutamate pathway blockade, a total of 228 differential metabolites (DMs) were identified in the three treatment groups. An enrichment analysis indicated significant involvement in glycerophospholipid metabolism, arachidonic acid metabolism, sphingolipid metabolism, purine metabolism, arginine and proline biosynthesis, pantothenate and CoA biosynthesis, glutathione metabolism, and fatty acid degradation, among other metabolic pathways. The results showed that ROS imbalances and L-arginine accumulation in crucian carp after the glutamate pathway blockade led to an increase in oxidative stress and inflammatory responses in vivo, which may cause damage to the structure and function of cell membranes. Crucian carp improves the body’s antioxidant capacity and regulates cellular homeostasis by activating glutathione metabolism and increasing the concentration of phosphatidylcholine (PC) analogs. Additionally, challenges such as aggravated ammonia excretion obstruction and disrupted energy metabolism were observed in crucian carp, with the upregulation of purine metabolism alleviating ammonia toxicity and maintaining energy homeostasis through pantothenate and CoA biosynthesis as well as fatty acid degradation. This study elucidated the metabolic changes in crucian carp under carbonate alkaline stress after a glutamate pathway blockade at the cellular metabolism level and screened out the key metabolic pathways, which provide a scientific basis for further in-depth studies on the ammonia excretion of freshwater scleractinian fishes under saline and alkaline habitats at a later stage.

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Metabolism responses in the intestine of Oreochromis mossambicus exposed to salinity, alkalinity and salt-alkalinity stress using LC-MS/MS-based metabolomics

Cited by 6 publications

References 101 publications

Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification

Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification

Unravelling the molecular mechanisms of fish salinity adaptation in the face of multiple stressors: A comparative multi-tissue transcriptomic study in the Llobregat River, Barcelona, Spain

Investigating the Impact of Disrupting the Glutamine Metabolism Pathway on Ammonia Excretion in Crucian Carp (Carassius auratus) under Carbonate Alkaline Stress Using Metabolomics Techniques

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