Metabolism of zinc in the mussel, <i>Mytilus edulis</i> (L.): a combined ultrastructural and biochemical study

George, Stephen; Pirie, B. J. S.

doi:10.1017/s0025315400040273

Cited by 136 publications

(28 citation statements)

References 21 publications

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“…In this specific case, it seems that the kidneys, saturated with Cr, were unable to maintain their excretory function and were adopting a storage function. Our results are similar to those of George & Pirie (1980) who concluded that the kidney forms the major storage organ for zinc.…”

Section: Discussionsupporting

confidence: 92%

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Chromium uptake, distribution and loss in the mussel Mytilus edulis a structural ultrastructural and microanalytical study

Chassard-Bouchaud¹,

Boutin²,

Hallégot³

et al. 1989

Dis. Aquat. Org.

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Several microanalytical techniques were used to assess the distribution and chemical form of chromium in various tissues of Mytjlus edulls, at the cellular and subcellular levels After 2 wk exposure to Cr (111) salt (Cr occurs principally in the trivalent state in the natural environment), investigations were performed on mussels, using secondary ion mass spectrometry (ion microscope and ion microprobe) associated with photon microscope, and X-ray spectrometry (electron microprobe) associated with transmission electron miscroscope. Cr measurements gave the following: kidney and gills exhibited the highest values, intermediate values were found for muscle and byssus (where Cr was adsorbed onto the threads and was incorporated within them), and lowest values were found in the digestive gland. Cr was also detected in the amoebocytes but not in the reproductive cells. The target organelle of Cr accumulation was shown to be the lysosome where the metal was associated with phosphorus and sulfur and trapped in an insoluble form. The significance of these different data is discussed. Cr kinetics of metabolism was compared between mussels and other aq.uatic organisms. Comparison in M. edulis of Cr metabolism with metabolism of other metals leads to the conclusion that Cr is metabolised and transported differently from most toxic metals.

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Section: Discussionsupporting

confidence: 92%

“…Our results are in agreement with this concept, according to which granular amoebocytes perform detoxication of metals, such as iiac, iii the oysier Zstrea eduks (George et G!. 1978) and in the mussel Mytilus edulis (George & Pirie 1980).…”

Section: Discussionsupporting

confidence: 88%

Chromium uptake, distribution and loss in the mussel Mytilus edulis a structural ultrastructural and microanalytical study

Chassard-Bouchaud¹,

Boutin²,

Hallégot³

et al. 1989

Dis. Aquat. Org.

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“…13 In species of Mytilus , metals are likely to be absorbed both from solution and from ingested phytoplankton and other suspended particles. 43 The common mussel, Perna perna, which selects its ingested food by the particulate size, from 1 μm to 4 mm, showed a higher capacity to accumulate mercury than the other bivalves (mangrove oyster, clam).…”

Section: Resultsmentioning

confidence: 99%

Total and methyl mercury in different species of molluscs from two estuaries in Rio de Janeiro State

Kehrig¹,

Costa²,

Moreira³

et al. 2006

J. Braz. Chem. Soc.

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Mercúrio total e metilmercúrio foram avaliados em três espécies de moluscos (Perna pernamexilhão, Crassostrea rhizophorae -ostra, Anomalocardia brasiliana -vôngole) provenientes de dois estuários no estado do Rio de Janeiro, impactados por matéria orgânica e metais pesados. O mexilhão foi a espécie que apresentou melhor capacidade para acumular o mercúrio dentre as espécies de molusco estudadas. Foi observada uma diferença significante nas concentrações de mercúrio entre os organismos de mexilhão, fêmeas e machos (81 ± 1 μg kg -1 e 70 ± 5 μg kg -1 em peso seco respectivamente), com comprimento de concha semelhante. Entretanto, não foi observada diferença significante na percentagem de metilmercúrio entre fêmeas (64%) e machos (63%) de mexilhão. Apesar de possuírem o hábito alimentar semelhante, o mexilhão apresentou concentrações de mercúrio total e metilmercúrio superiores (76 ± 7 μg kg -1 e 48 ± 5 μg kg -1 peso seco) às da ostra (19 ± 4 μg kg -1 e 6 ± 1 μg kg -1 peso seco). Este fato pode estar relacionado com diferenças na capacidade em selecionar o tamanho de partículas e os alimentos ingeridos; também, estar refletindo a maior habilidade do mexilhão em concentrar e excretar o metilmercúrio dos seus tecidos, bem como refletindo as condições ambientais.The paper assesses methyl and total mercury concentrations in three mollusc species (Perna perna -common mussel, Crassostrea rhizophorae -mangrove oyster, Anomalocardia brasiliana -clam) from two estuaries in Rio de Janeiro State, both impacted by organic matter and heavy metals. Mussels showed higher capacity to accumulate mercury compared to the other mollusc species (oyster, clam). A significant difference was observed between mercury concentration in the female mussel organisms and in males (81 ± 1 μg kg -1 dry wt. and 70 ± 5 μg kg -1 dry wt. respectively) with similar total shell length. No significant difference was observed among the % MeHg in the female (64%) and male (63%) mussel organisms. Even though the feeding habits of the molluscs are similar; mussels presented higher mercury and methylmercury concentrations in their soft tissues (76 ± 7 μg kg -1 dry wt. and 48 ± 5 μg kg -1 dry wt.) than oysters (19 ± 4 μg kg -1 dry wt. and 6 ± 1 μg kg -1 dry wt.). This is possible related to their capacity to select particle size and the composition of the ingested food they assimilate, and also reflects the greater ability of mussels to concentrate and excrete methylmercury and also to reflect their environmental conditions.

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“…The gills of aquatic molluscs constitute a key interface for the uptake of dissolved metal ions from water George and Pirie, 1980;Carpene and George, 1981;Roesijadi, 1982;Jeantet et al, 1985;Hietanen et al, 1988;Viarengo, 1989;Everaarts, 1990;Roesijadi and Unger, 1993;Soto et al, 1996b). Cadmium influx in the bivalve Mytilus edulis is via the gills, and to a lesser extent the mantle, the primary uptake being negligible via the digestive gland (Scholz, 1980;Janssen and Ertelt-Janssen, 1983).…”

Section: Gillsmentioning

confidence: 99%

“…Once metals enter the organism, defense mechanisms include the participation of hemocytes and pore/ brown cells in transporting metal ions toward detoxification organs (George, 1980;George and Pirie, 1980;Robinson and Ryan, 1988;Marigómez et al, 1990a,b, Roesijadi andRobinson, 1993). Under metal exposure conditions (Cd, Cu, Pb), numerous hemocytes migrate towards gills or digestive tract and other detoxification organs (Benyahia et al, 1988;Giamberini et al, 1996b).…”

Section: Cell-mediated Transportmentioning

confidence: 99%

Cellular and subcellular distribution of metals in molluscs

Marigómez

Soto

Cajaraville

et al. 2002

Microscopy Res & Technique

420

202

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The cellular processes involved in metal metabolism in molluscs are reviewed, with emphasis on the contribution of microscopy (AMG, ARG, EPMA, and SIMS) to both basic research of metal cell biology and applied environmental research. In molluscs, metal uptake may occur by facilitated diffusion, active transport, or endocytosis, and can be enhanced by MT synthesis or formation of mineralized granules. In aquatic molluscs, gills constitute a key interface for dissolved metal uptake, where metals are bound to MT, incorporated into lysosomes, and released basally towards the blood plasma and circulating hemocytes. However, particulate metal uptake is mainly achieved via the digestive tract by endocytosis; further metals are transferred first to lysosomes and then to residual bodies, especially in the digestive cells of the digestive gland. Additionally, metals can be accumulated selectively in specific cell types. As ligands pools differ from cell to cell, different metals may be retained in different cell types. Class "a" metals are localized in cells with granules composed of carbonate, oxalate, phosphate, and sulfate (oxygen donors), whereas "b" metals are associated with those cell types rich in sulfur and nitrogen ligands (sulfur donors). In molluscs, oxygen donors occur in connective tissue calcium cells and basophilic cells, whereas sulfur donors are present in digestive cells, podocytes, nephrocytes, and rhogocytes. Hemocytes, which constitute the most relevant system for metal transport between tissues, move around the body and may penetrate tissues and remove metals from the inner medium to be accumulated in lysosomes as nondigested products. Rhogocytes also participate in metal mobilization, accumulation, and release. The assessment of metal levels in target cells of sentinel molluscs by microscopic techniques provides an early-warning measure, with promising applications as an exposure biomarker for environmental monitoring programs.

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Metabolism of zinc in the mussel, Mytilus edulis (L.): a combined ultrastructural and biochemical study

Cited by 136 publications

References 21 publications

Chromium uptake, distribution and loss in the mussel Mytilus edulis a structural ultrastructural and microanalytical study

Chromium uptake, distribution and loss in the mussel Mytilus edulis a structural ultrastructural and microanalytical study

Total and methyl mercury in different species of molluscs from two estuaries in Rio de Janeiro State

Cellular and subcellular distribution of metals in molluscs

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