Metabolism of Tryptophan by Pseudomonas aureofaciens and Its Relationship to Pyrrolnitrin Biosynthesis

Salcher, Olga; Lingens, Franz

doi:10.1099/00221287-121-2-465

Cited by 13 publications

(11 citation statements)

References 19 publications

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“…Trp has also been implicated as the sole source of carbon and nitrogen in some species of Pseudomonadaceae and Bacillaceae [36][37][38][39][40]. The resumption of growth after an intervening stationary phase when Trp was present in Fig.…”

Section: Discussionmentioning

confidence: 99%

Growth and indole-3-acetic acid biosynthesis ofAzospirillum brasilenseSp245 is environmentally controlled

Ona

Impe

Prinsen

et al. 2005

FEMS Microbiology Letters

102

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Batch and fed batch cultures of Azospirillum brasilense Sp245 were conducted in a bioreactor. Growth response, IAA biosynthesis and the expression of the ipdC gene were monitored in relation to the environmental conditions (temperature, availability of a carbon source and aeration). A. brasilense can grow and produce IAA in batch cultures between 20 and 38 degrees C in a standard minimal medium (MMAB) containing 2.5 gl(-1)l-malate and 50 microgml(-1) tryptophan. IAA synthesis requires depletion of the carbon source from the growth medium in batch culture, causing growth arrest. No significant amount of IAA can be detected in a fed batch culture. Varying the concentration of tryptophan in batch experiments has an effect on both growth and IAA synthesis. Finally we confirmed that aerobic growth inhibits IAA synthesis. The obtained profile for IAA synthesis coincides with the expression of the indole-3-pyruvate decarboxylase gene (ipdC), encoding a key enzyme in the IAA biosynthesis of A. brasilense.

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Section: Discussionmentioning

confidence: 99%

Growth and indole-3-acetic acid biosynthesis ofAzospirillum brasilenseSp245 is environmentally controlled

Ona

Impe

Prinsen

et al. 2005

FEMS Microbiology Letters

102

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“…Excretion of the antifungal compound pyrrolnitrin by bacteria was studied by TLC as described earlier (Salcher & Lingens, 1980). Bacterial cells grown on NA supplemented with 2.0% (v/v) glycerine were scrapped from the medium after cultivation for 3 days at 30 °C and suspended in 6.0 mL distilled water.…”

Section: Methodsmentioning

confidence: 99%

Quorum-sensing effects in the antagonistic rhizosphere bacterium Serratia plymuthica HRO-C48

Müller

Westendorf

Leitner

et al. 2009

FEMS Microbiology Ecology

125

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The rhizosphere-associated bacterium Serratia plymuthica HRO-C48 is not only able to suppress symptoms caused by soil-borne pathogens but is also able to stimulate growth of plants. Detailed knowledge about the underlying mechanisms and regulation are crucial for the application in biocontrol strategies. To analyse the influence of N-acyl homoserine lactone (AHL)-mediated communication on the biocontrol activity, the AHL-degrading lactonase AiiA was heterologously expressed in the strain, resulting in abolished AHL production. The comparative analysis of the wild type and AHL negative mutants led to the identification of new AHL-regulated phenotypes. In the pathosystem Verticillium dahliae-oilseed rape, the essential role of AHL-mediated signaling for disease suppression was demonstrated. In vitro, the regulatory function of AHLs in the synthesis of the plant growth hormone indole-3-acetic acid is shown for the first time. Additionally, swimming motility was found to be negatively AHL regulated. In contrast, production of extracellular hydrolytic enzymes is shown to be positively AHL-regulated. HRO-C48 emits a broad spectrum of volatile organic compounds that are involved in antifungal activity and, interestingly, whose relative abundances are influenced by quorum sensing (QS). This study shows that QS is crucial for biocontrol activity of S. plymuthica and discusses the impact for the application of the strain as a biocontrol agent.

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“…Interestingly, the PcO6 gacS mutant was auxotrophic for tryptophan, because transcription of trpE, encoding a key tryptophan biosynthetic enzyme, was significantly reduced in the gacS mutant (Kim et al, 2014a). Tryptophan is the key precursor for biosynthesis of IAA (Spaepen and Vanderleyden, 2011) and pyrrolnitrin (Salcher and Lingens, 1980), and tryptophan is derived from chorismate, which is known as the precursor of phenazines (Blankenfeldt and Parsons, 2014). Consequently, Gac/Rsmregulated metabolism is linked to the biosynthetic pathways of several intermediates that are important for the biocontrol potential of PcO6.…”

Section: Traits Regulated Negatively By the Gac Regulonmentioning

confidence: 99%

The Gac/Rsm Signaling Pathway of a Biocontrol Bacterium, Pseudomonas chlororaphis O6

Anderson

Kang

Kim

2017

Research in Plant Disease

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Pseudomonas chlororaphis O6, isolated from the roots of dryland, field-grown commercial wheat in the USA, enhances plant health and therefore it is used in agriculture as a biofertilizer and biocontrol agent. The metabolites produced by this pseudomonad stimulate plant growth through direct antagonism of pathogens and by inducing systemic resistance in the plant. Studies upon P. chlororaphis O6 identify the pathways through which defined bacterial metabolites generate protection against pathogenic microbes, insects, and nematodes. P. chlororaphis O6 also triggers plant resistance to drought and salinity stresses. The beneficial determinants are produced from bacterial cells as they form biofilms during root colonization. Molecular control these processes in P. chlororaphis O6 involves the global regulatory Gac/Rsm signaling cascade with cross-talk between other global regulatory pathways. The Gac/Rsm regulon allows for coordinate phasing of expression of the genes that encode these beneficial traits among a community of cells. This review provides insights on the Gac/Rsm regulon in expression of beneficial traits of the P. chlororaphis O6 which can contribute to help yield enhancement and quality in agricultural production.

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Metabolism of Tryptophan by Pseudomonas aureofaciens and Its Relationship to Pyrrolnitrin Biosynthesis

Cited by 13 publications

References 19 publications

Growth and indole-3-acetic acid biosynthesis ofAzospirillum brasilenseSp245 is environmentally controlled

Growth and indole-3-acetic acid biosynthesis ofAzospirillum brasilenseSp245 is environmentally controlled

Quorum-sensing effects in the antagonistic rhizosphere bacterium Serratia plymuthica HRO-C48

The Gac/Rsm Signaling Pathway of a Biocontrol Bacterium, Pseudomonas chlororaphis O6

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