Metabolism of Parathion by Two Species of Rhizobium1

Mick, David Lee; Dahm, Paul A.

doi:10.1093/jee/63.4.1155

Cited by 35 publications

(4 citation statements)

References 60 publications

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“…Rhizobium spp. were able to produce phosphotriesterases and phosphodiesterases and were found to degrade OP triesters including parathion and fenitrothion …”

Section: Resultsmentioning

confidence: 99%

“…were able to produce phosphotriesterases and phosphodiesterases 52 and were found to degrade OP triesters including parathion and fenitrothion. 53 Functional genes associated with 2-chloroethanol (2-CE) metabolism, which is another possible metabolite of TCEP degradation, were significantly higher in microcosms with TCEP addition than those with acetate addition (Welch's ttest, p < 0.05) (Figure 5). Those functional genes include genes coding for enzymes catalyzing the conversion of 2chloroethanol to chloroacetaldehyde (alcohol dehydrogenase K00114, methanol dehydrogenase K14028 and K14029), chloroacetaldehyde to choroacetate (aldehyde dehydrogenase K00128), and chloroacetate to glycolate (haloacetate dehalogenase K01561 and 2-haloacid dehalogenase K01560) (Figure 5).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Biotransformation of Tris(2-chloroethyl) Phosphate (TCEP) in Sediment Microcosms and the Adaptation of Microbial Communities to TCEP

Zhou

Liang

Ren

et al. 2020

Environ. Sci. Technol.

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Tris(2-chloroethyl) phosphate (TCEP), a typical chlorinated organophosphate ester (OPE), is an emerging contaminant of global concern because of its frequent occurrence, potential toxic effects, and persistence in the environment. In this study, we investigated the microbial TCEP biotransformation and the development of microbial communities in sediment microcosms with repeated TCEP amendments. The TCEP degradation fitted pseudo-zero-order kinetics, with reaction rates of 0.068 mg/(L h) after the first spike of 5 mg/L and 1.85 mg/(L h) after the second spike of 50 mg/L. TCEP was mainly degraded via phosphoester bond hydrolysis, evidenced by the production of bis(2-chloroethyl) phosphate (BCEP) and mono-chloroethyl phosphate (MCEP). Bis(2-chloroethyl) 2-hydroxyethyl phosphate (TCEP-OH), phosphoric bis(2-chloroethyl) (2-oxoethyl) ester (TCEP-CHO), phosphoric acid bis(2-chloroethyl)(carboxymethyl) ester (TCEP-COOH), and 2-chloroethyl 2-hydroxyethyl hydrogen phosphate (BCEP-OH) were also identified as microbial TCEP transformation products, indicating that TCEP degradation may follow hydrolytic dechlorination and oxidation pathways. Microbial community compositions in TCEP-amended microcosms shifted away from control microcosms after the second TCEP spike. Burkholderiales and Rhizobiales were two prevalent bacterial guilds enriched in TCEP-amended microcosms and were linked to the higher abundances of alkaline and acid phosphatase genes and genes involved in the metabolism of 2-chloroethanol, a side product of TCEP hydrolysis, indicating their importance in degrading TCEP and its metabolites.

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“…Rhizobium spp. were able to produce phosphotriesterases and phosphodiesterases and were found to degrade OP triesters including parathion and fenitrothion …”

Section: Resultsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Biotransformation of Tris(2-chloroethyl) Phosphate (TCEP) in Sediment Microcosms and the Adaptation of Microbial Communities to TCEP

Zhou

Liang

Ren

et al. 2020

Environ. Sci. Technol.

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“…Aminoparathion has been reported to be a degradation product of parathion in flooded soils (Sethunathan and Yoshida, 1973; Katan et al, 1976), in lake sediments (Graetz et al, 1970), in a simulated cranberry bog (Miller et al, 1966), in a loam soil and inoculated soil water (Lichtenstein and Schulz, 1964), and in cultures of Rhizobium (Mick and Dahm, 1970), soil bacteria (Graetz et al, 1970), Penicillium (Rao and Sethunathan, 1974), and of Chlorella (Zuckerman et al, 1970). It was suggested that aminoparathion, formed in cells of Chlorella, does not accumulate and is rapidly released into the culture medium.…”

Section: Binding Of Potential Metabolites Ofmentioning

confidence: 99%

Mechanisms of production of soil-bound residues of [14C]-parathion by microorganisms

Katan¹,

Lichtenstein²

1977

J. Agric. Food Chem.

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“…According to them, a high soil moisture content and the presence of soil microflora favored the degradation of parathion in soils. The major pathway of parathion breakdown in soils (Lichtenstein and Schultz, 1964), lake sediments (Graetz et al, 1970), and microorganisms in pure culture (Mick and Dahm, 1970;Zukerman et al, 1970) involves nitro reduction to its amino compound, aminoparathion (O, O-diethyl O-p-aminophenyl phosphorothioate).…”

mentioning

confidence: 99%