Metabolism of Lactic Acid Containing Radioactive Carboxyl Carbon

Tracer isotopes in biochemistry

“…Interesting carbohydrate experiments have been carried out with C11. About two hours were consumed in preparing a sodium lactate labeled with this isotope (25). The material was fed to rats for 2.5 hours before they were killed.…”

Gluconeogenesis: A teaching pathway. Criteria to establish a metabolic pathway

“…pathways is supported by some experiments that can not be explained by a direct phosphorylation of pyruvate to PEP via the pyruvate kinase reaction. It has been shown that kidney cortex extracts catalyze the conversion of fumarate and malate to PEP (6) and that less than 2% of lactate carboxyl carbon is converted to liver glycogen (7,8). These data suggested that only carbon 2 and 3 of the lactate molecule participate to PEP synthesis from pyruvate and that dicarboxylic acids are metabolic intermediates.…”

Chiral nickel(ii) complexes in the preparation of11C- and18F-labelled enantiomerically pure α-amino acids

“…[ 11 C]lactic acid was employed for evaluation of its in vivo metabolism. 11 The first synthesis of an [ 11 C]amino acid was published in 1973. 12 Racemic [ 11 C]amino acids have been separated to enantiomers by means of chromatography on a chiral stationary phase or enzymes.…”

“…16 Stoichiometric chiral auxiliaries and chiral catalysts for the creation of a chiral centre are seldom used. 17 For broadening the scope of available tools for PET labelling it is important to develop new approaches for the creation of a quaternary asymmetric centre via alkylation of tertiary carbanions by 11 CH 3 I or 11 CH 3 OTf and for the asymmetric synthesis of clinically valuable amino acids starting with easily available 18 F -.…”