Metabolism of immunoglobulin A in lactating mice: origins of immunoglobulin A in milk

Halsey, John F.; Mitchell, Craig; Meyer, R R; Cebra, John J.

doi:10.1002/eji.1830120203

Cited by 41 publications

(28 citation statements)

References 29 publications

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“…Another possibility could reside in the inappropriate time of collection of bile and plasma used to compare their specific activities. Others have also reported higher (1.5 times) specific activities in mouse milk than in mouse serum for injected labeled pIgA [67]. The same phenomenon was also reported for transfer of pIgA in sheep bile [55].…”

Section: Discussionsupporting

confidence: 67%

Hepatobiliary transport of plasma IgA in the mouse: contribution to clearance of intravascular IgA

1985

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Labeled monomeric and polymeric (pIgA) mouse monoclonal IgA were injected intravenously into mice which were either sequentially bled for plasma turnover studies of IgA, or cannulated at their common bile duct, with excluded gallbladder, for quantitation of plasma-to-bile transport of pIgA. Our data show that mice do display a relatively high rate of biliary transport of plasma pIgA (22-28% of the injected 125I-labeled pIgA over 3 h), which accounts for approximately 90% of the total amount of pIgA (8.8 mg/kg/day) daily delivered by hepatic bile into the duodenal fluid of this species. However, in mice the absolute biliary output of pIgA does not exceed that of IgG (9.5 mg/kg/day) and the kinetics of the hepatobiliary transport of plasma pIgA appear to be slower than in the rat. Furthermore, as plasma survival studies of 125I-labeled pIgA yielded a plasma turnover of pIgA averaging 20.6 mg/kg/day, it can be approximated that the hepatobiliary pathway contributes for only 38% to the elimination of intravascular pIgA from mouse plasma, a figure to be compared to 89.8% in the rat and approximately 8.9% in man. We conclude that internal catabolism plays a dominant role in the clearance of intravascular pIgA in the mouse which appears as a model intermediate between rats and humans. Supporting this conclusion, serum pIgA two days after common bile duct ligation in 6 mice was increased by 2.5-fold vs. greater than 14-fold in ligated rats and 1.1-fold in humans with complete biliary obstruction.

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Section: Discussionsupporting

confidence: 67%

Hepatobiliary transport of plasma IgA in the mouse: contribution to clearance of intravascular IgA

1985

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“…Much higher values have been reported for rodent milk. These range from 5 mg/ml for rats (Jordan & Morgan, 1967;Grigor, Allan, Carne, Carrington & Geursen, 1986) to over 20 mg/ml for mice (Halsey, Mitchell, Meyer & Cebra, 1982). This latter value approaches the concentration of albumin found in serum of lactating mice.…”

Section: Introductionmentioning

confidence: 70%

Serum albumin secretion in rat milk.

Geursen

Grigor

1987

The Journal of Physiology

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SUMMARY1. The concentration ofserum albumin in rat milk, rat serum, the stomach contents of suckling rats and mammary homogenates has been measured.2. Serum albumin in expressed milk ranges in concentration between 3-5 and 6 mg/ml and amounts to an average of 4-5 mg/ 100 mg total protein whereas, in the stomach contents of suckling rats, serum albumin was 3-2 mg/100 mg total protein.3. Corresponding concentrations of a second soluble milk protein, a-laetalbumin, were 2-9 mg/100 mg total protein in the milk and 1-2 mg/100 mg total protein in the stomach contents.4. Serum albumin is a major protein in mammary homogenates at 10 mg/100 mg total soluble protein and the calculated total mammary pool of albumin amounts to 110 mg/rat.5. There was a significant negative correlation between the serum albumin concentration and the ratio of potassium to sodium ions in milk samples obtained from rats milked with varying oxytocin treatments.6. No evidence for albumin mRNA could be detected when mammary total RNA was probed with a complementary DNA (cDNA) for rat albumin.7. The data are consistent with albumin being a major whey protein in the rat, it being synthesized at an extramammary site and transferred to the milk space by a paracellular mechanism from an extravascular mammary pool rather than directly from the serum.

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“…Throughout lactation, mice source milk IgA from distant mucosal sites. Immediately postpartum, this is achieved by seconding IgA at mucosal surfaces into the serum, which results in a rapid increase in circulating polymeric IgA that permits loading of IgA into the milk via the blood, such that all IgA in milk from 4-day lactating dams is serum derived (26,27). By around day 8 of lactation, substantial numbers of mucosally derived IgA plasma cells have migrated to the mammary glands to establish local production, such that the proportion of serum-derived IgA decreases to below 25% of the total milk IgA (57,60,61).…”

Section: Discussionmentioning

confidence: 99%

Antibody-Mediated Protection against Infection withHelicobacter pyloriin a Suckling Mouse Model of Passive Immunity

Gorrell

Robins-Browne

2009

Infect Immun

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Studies of active immunization againstHelicobacter pylori indicate that antibodies play a minor role in immunity. There is also evidence, however, that the translocation of antibodies in the stomach may be insufficient to achieve functional antibody levels in the gastric lumen. We have used a suckling mouse model of passive immunity to determine if perorally delivered antibodies can protect against infection with H. pylori. Female C57BL/6 mice were immunized parenterally with formalin-fixed cells of three clinical isolates of H. pylori (3HP) or the mouse-adapted H. pylori strain SS1 before mating. Their pups were challenged with the SS1 strain at 4 days of age and left to suckle before determination of bacterial loads 14 days later. Compared to age-matched controls, pups suckled by 3HP-vaccinated dams were significantly protected against infection (>95% reduction in median bacterial load; P < 0.0001). Pups suckled by SS1-vaccinated dams were also significantly protected in terms of both median bacterial load (>99.5% reduction; P < 0.0001) and the number of culture-negative pups (28% versus 2% for immune and nonimmune cohorts, respectively; P < 0.0001). Similar results were obtained with pups suckled by dams immunized with a urease-deficient mutant of SS1. Fostering experiments demonstrated that protection was entirely attributable to suckling from an immunized dam, and antibody isotype analysis suggested that protection was mediated by the immunoglobulin G fraction of immune milk. Analysis of the bacterial loads in pups sampled before and after weaning confirmed that infection had been prevented in culture-negative animals. These data indicate that antibodies can prevent colonization by H. pylori and suppress the bacterial loads in animals that are colonized.

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Metabolism of immunoglobulin A in lactating mice: origins of immunoglobulin A in milk

Cited by 41 publications

References 29 publications

Hepatobiliary transport of plasma IgA in the mouse: contribution to clearance of intravascular IgA

Hepatobiliary transport of plasma IgA in the mouse: contribution to clearance of intravascular IgA

Serum albumin secretion in rat milk.

Antibody-Mediated Protection against Infection withHelicobacter pyloriin a Suckling Mouse Model of Passive Immunity

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