Metabolism of Glutamic Acid in Aspergillus ochraceus During the Biosynthesis of Ochratoxin A

Bacon, Charles W.; Robbins, J. D.; Burdick, D.

doi:10.1128/aem.29.3.317-322.1975

Cited by 12 publications

(4 citation statements)

References 15 publications

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“…This increased production of citrinin associated with a reduction of red pigments is actually in accordance with the fact that both metabolites aroused from the same metabolite precursors. This result is also in agreement with a previous work showing that glutamate and proline stimulated ochratoxin in Aspergillus ochraceus (Bacon and others 1975; Haggblom and Ghoch 1985). Also, proline has been described as positive effect on aflatoxin production in A. parasiticus and A. flavus (Payne and Hagler, 1983).…”

Section: Resultssupporting

confidence: 94%

Effect of Amino Acids on Red Pigments and Citrinin Production in Monascus ruber

Hajjaj¹,

François²,

Goma³

et al. 2012

Journal of Food Science

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Amino acids were used as sole nitrogen sources to examine their effects on the production of water-soluble red pigments and citrinin by Monascus ruber ATCC 96218 cultivated on chemically defined media. In general, when glycine, tyrosine, arginine, serine, or histidine were used as sole nitrogen sources, they favored the production of red pigments, and restricted the synthesis of the mycotoxin. In contrast, the production of citrinin was enhanced in media supplemented with either glutamate, alanine, or proline. Histidine was found to be the most valuable amino acid as it resulted in the highest production of red pigments and almost completely eliminated the formation of mycotoxin.

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Section: Resultssupporting

confidence: 94%

Effect of Amino Acids on Red Pigments and Citrinin Production in Monascus ruber

Hajjaj¹,

François²,

Goma³

et al. 2012

Journal of Food Science

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“…Furthermore, glutamic acid is incorporated in both the isocoumarin ring and phenylalanine of OTA by A . ochraceus [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius

et al. 2016

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Ochratoxin A (OTA) is a mycotoxin harmful for animals and humans. Aspergillus carbonarius is the main responsible for OTA contamination of grapes and derived products. Gene transcriptional profiling of 4 A. carbonarius strains was carried out by RNA-Seq analysis to study transcriptome changes associated with OTA production. By comparing OTA inducing (OTAI) vs. non-inducing (OTAN) cultural conditions, a total of 3,705 differentially expressed genes (DEGs) (fold change > |2| and FDR ≤ 0.05) were identified. Several genes involved in primary metabolic processes, with particular regard to carbohydrate and amino acid metabolisms, secondary metabolic processes, transport, response to stress and sporulation were up-regulated by OTAI conditions at all the analysed sampling times (4, 6 and 8 DAI) or starting from 6 DAI. Highly up-regulated DEGs encoding enzymes involved in biosynthesis of secondary metabolites, oxidoreductases, transporters and transcription factors were examined for their potential involvement in OTA biosynthesis and related metabolic pathways. Differential expression of genes encoding polyketide synthases (pks), non-ribosomal peptide synthetases (nrps) and chloroperoxidase (cpo) was validated by RT-qPCR. Among clusters of co-regulated genes involved in SM biosynthesis, one putative OTA-gene cluster, including both pks and nrps genes, was detected in the A. carbonarius genome.

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“…Ferreira (6) reported that glutamic acid was indirectly involved in the production of ochratoxin A in shaken culture and that proline could be substituted. A study of the metabolism of glutamic acid during the production of ochratoxin A indicated that portions of it were incorporated into the mycotoxin (1). The production of F-2 followed an increase in pH of the medium, which may represent the deamination of glutamic acid.…”

Section: Concmentioning

confidence: 99%

Media for identification of Gibberella zeae and production of F-2-(Zearalenone)

Bacon¹,

Robbins²,

Porter³

1977

Appl Environ Microbiol

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Media are described for the isolation of Fusarium graminearum in the perithecial state, Gibberella zeae, and for the production of F-2 (zearalenone) by Fusarium species. On soil extract-corn meal agar isolation medium, G. zeae produced perithecia in 9 to 14 days under a 12-h phot'operiod. Species of Fusarium were screened for F-2 production on a liquid medium. From strains that produced F-2, the yields, from stationary cultures of G. zeae and F. culmorum after 12 days of incubation, ranged from 22 to 86 mg/liter. Three strains produced no F-2. Glutamic acid, starch, yeast extract, and the proper ratio of medium volume-to-flask volume were necessary for F-2 synthesis.

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Metabolism of Glutamic Acid in Aspergillus ochraceus During the Biosynthesis of Ochratoxin A

Cited by 12 publications

References 15 publications

Effect of Amino Acids on Red Pigments and Citrinin Production in Monascus ruber

Effect of Amino Acids on Red Pigments and Citrinin Production in Monascus ruber

RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius

Media for identification of Gibberella zeae and production of F-2-(Zearalenone)

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