Metabolism of glucose by unicellular blue-green algae

Pelroy, R.A.; Rippka, Rosmarie; Stanier, Roger Y.

doi:10.1007/bf00409131

Cited by 162 publications

(97 citation statements)

References 27 publications

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“…Our CO2 fixation studies suggested the efficacy of this approach. The stimulation of CO2 fixation in Anabaena by glucose-6-P is promising since this compound has been implicated as an important branch point intermediate in blue-green algae, with its turnover being controlled by some product(s) of photosynthesis (6,16). Our goal, therefore, is to demonstrate that glucose-6-P enters Anabaena cells intact, turns over in a manner consistent with previous studies on in vivo pool size changes (8,13), and affects several physiological functions.…”

Section: Methodsmentioning

confidence: 99%

“…Although their mode of photosynthesis seems to be essentially identical to that of eucaryotic organisms (9), a blocked tricarboxylic acid cycle and a limited glycolytic cycle, due to the absence or low levels of phosphofructokinase (11,12,16,18), suggest that carbon flow in these organisms is either by a catabolic pentose phosphate shunt, as shown by Cheung and Gibbs (5), or by direct utilization of triose produced during photosynthetic carbon reduction. The questionable functioning of glycolysis and the tricarboxylic acid cycle, and the concomitant lack of ATP synthesis in the dark, might be the reason for limited growth on glucose or acetate (11,12,16).…”

Section: Atp In Blueen Algementioning

confidence: 99%

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Uptake and Utilization of Sugar Phosphates by Anabaena flos-aquae

Rubin

Zetooney²,

McGowan³

1977

Plant Physiol.

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The effect of various sugar phosphates on CO2 fixation in Anabacna flos-aquae was investigated and found to be very smilar to that found for isolated spinch chloroplasts. One exception, glucose 6-phosphate, has a stimulatory effect on CO2 fixation in Anabena but not in isolated chloroplast.Further examination of the role of glucose 6-phosphate metabolsm in The generally obligate nature of photoautotrophic growth in the blue-green algae has prompted the investigation of the biochemical basis for this growth habit. Although their mode of photosynthesis seems to be essentially identical to that of eucaryotic organisms (9), a blocked tricarboxylic acid cycle and a limited glycolytic cycle, due to the absence or low levels of phosphofructokinase (11,12,16,18), suggest that carbon flow in these organisms is either by a catabolic pentose phosphate shunt, as shown by Cheung and Gibbs (5), or by direct utilization of triose produced during photosynthetic carbon reduction. The questionable functioning of glycolysis and the tricarboxylic acid cycle, and the concomitant lack of ATP synthesis in the dark, might be the reason for limited growth on glucose or acetate (11,12,16).Recently, Pelroy and , have shown that turnover of glucose-6-P is restricted in the light and that rapid turnover occurs in the dark. Grossman and McGowan (6) have investigated the kinetics of glucose-6-P dehydrogenase in Anacystis and Anabaena and have determined regulation of the enzyme's catalytic activity by pH, NADPH, and ATP. These data suggest that if glucose-6-P could be assimilated in the dark, its turnover via the pentose phosphate pathway should go unhindered. However, it is generally believed that sugar phosphates are not assimilated by whole algal cells, or that if they are, they are first dephosphorylated at the cell membrane.In this study we present data which suggest that some sugar

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Section: Methodsmentioning

confidence: 99%

Section: Atp In Blueen Algementioning

confidence: 99%

Uptake and Utilization of Sugar Phosphates by Anabaena flos-aquae

Rubin

Zetooney²,

McGowan³

1977

Plant Physiol.

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“…19). In the dark, the endogenous glycogen reserve is mobilised and respired through the oxidative pentose phosphate cycle (6,31), as shown in fig. 20.…”

Section: Cyanobacterial Carbon Metabolism and The Phenomenon Of Obligmentioning

confidence: 99%

“…20. The two dehydrogenases of this metabolic pathway, glucose-6-phosphate and 6-phosphogluconate dehydrogenases, are both NADP-specific in cyanobacteria (31). Dark ATP synthesis is thus associated with the reoxidation of NADPH via an 05-linked respiratory transport chain (3), the components of which have not so far been characterized.…”

Section: Cyanobacterial Carbon Metabolism and The Phenomenon Of Obligmentioning

confidence: 99%

The position of cyanobacteria in the world of phototrophs

Stanier

1977

Carlsberg Res. Commun.

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Recent advances in our knowledge of the biological properties of the cyanobacteria (former ,blue-green algae,) are reviewed. The subjects discussed include: cyanobacterial cell structure and development; comparative aspects of photosyntetic structure and function in prokaryotes; carbon metabolism and its relation to obligate photoautotrophy; and special properties of cyanobacteria that are of ecological significance (temperature relationships, nitrogen fixation and facultative anoxygenic photosynthesis).

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“…This suggested the possibility that the high level of ribulose-1,5-diphosphate in the light might act to suppress the activity of gl ucose-6-phosphate dehydrogenase. Finally, Pe 1 roy, et al [10] found the glucose-6-phosphate dehydrogenase activity of several strains of blue-green algae to be inhibited by physiological levels of 4 9 7…”

Section: Introductionmentioning

confidence: 99%