Metabolism of fatty acid in yeast: Characterisation of Î²-oxidation and ultrastructural changes in the genus<i>Sporidiobolus</i>sp. cultivated on ricinoleic acid methyl ester

Féron, Gilles; Blin-Perrin, Caroline; Krasniewski, Isabelle; Lherminier, Jeannine

doi:10.1016/j.femsle.2005.06.045

Cited by 18 publications

(9 citation statements)

References 30 publications

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“…Acyl-CoA dehydrogenase (EC 1.3.99.3) was determined according to the procedure of Baltazar et al (1999) and Feron et al (2005). Total activity was determined in 1 mL of HEPES/KOH buffer (50 mmol/L, pH 8.0), and the reduction of 100 μmol of 2,6-dichlorophenolindophenol (DCPIP) was monitored at 600 nm in the presence of 50 μmol of phenazine metosulfate, 72 nmol of acyl CoA, and 200 μg of CFE.…”

Section: Acyl-coa Dehydrogenase Activitymentioning

confidence: 99%

Effects of metal ions on growth, β-oxidation system, and thioesterase activity of Lactococcus lactis

Li¹,

Ma²

2014

Journal of Dairy Science

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The effects of divalent metal ions (Ca(2+), Mg(2+), Fe(2+), and Cu(2+)) on the growth, β-oxidation system, and thioesterase activity of Lactococcus lactis were investigated. Different metal ions significantly influenced the growth of L. lactis: Ca(2+) and Fe(2+) accelerated growth, whereas Cu(2+) inhibited growth. Furthermore, Mg(2+) inhibited growth of L. lactis at a low concentration but stimulated growth of L. lactis at a high concentration. The divalent metal ions had significant effects on activity of the 4 key enzymes of the β-oxidation system (acyl-CoA dehydrogenase, enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, and thiolase) and thioesterase of L. lactis. The activity of acyl-CoA dehydrogenases increased markedly in the presence of Ca(2+) and Mg(2+), whereas it decreased with 1 mmol/L Fe(2+) or 12 mmol/L Mg(2+). All the metal ions could induce activity of enoyl-CoA hydratase. In addition, 12 mmol/L Mg(2+) significantly stimulated activity of L-3-hydroxyacyl-CoA dehydrogenase, and all metal ions could induce activity of thiolase, although thiolase activity decreased significantly when 0.05 mmol/L Cu(2+) was added into M17 broth. Inhibition of thioesterase activity by all 4 metal ions could be reversed by 2 mmol/L Ca(2+). These results help us understand the effect of metal ions on the β-oxidation system and thioesterase activity of Lactococcus lactis.

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Section: Acyl-coa Dehydrogenase Activitymentioning

confidence: 99%

Effects of metal ions on growth, β-oxidation system, and thioesterase activity of Lactococcus lactis

Li¹,

Ma²

2014

Journal of Dairy Science

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“…While glycerol is converted to dihydroxyacetone phosphate, free fatty acids are activated by esterification with coenzyme A, followed by their oxidative catabolism (β‐oxidation), utilization in the synthesis of complex lipids or as an attachment to certain proteins (McIlhinney et al ., ; Daum et al ., ). Different organisms have adopted distinct β‐oxidation strategies to utilize fatty acids (Kunau et al ., ; Hashimoto, ; Wanders et al ., ; Feron et al ., ). Although β‐oxidation of fatty acids occurs primarily in mitochondria, catabolism through peroxisomal β‐oxidation is also well known in mammals (Kunau et al ., ; Hashimoto, ; Wanders et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…In fungi, owing to extensive studies in yeasts, it was generally accepted that the β‐oxidation of fatty acids was exclusive to the peroxisomes (Hiltunen et al ., ). However, previous studies have demonstrated the presence of a mitochondrial β‐oxidation pathway in Sporidiobolus pararoseus (Feron et al ., ) and in the filamentous fungus Aspergillus nidulans (Maggio‐Hall and Keller, ; Hynes et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial β‐oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae

Patkar

Ramos‐Pamplona²,

Gupta

et al. 2012

Molecular Microbiology

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SummaryFatty acids stored as triglycerides, an important source of cellular energy, are catabolized through b-oxidation pathways predicted to occur both in peroxisomes and mitochondria in filamentous fungi. Here, we characterize the function of Enoyl-CoA hydratase Ech1, a mitochondrial b-oxidation enzyme, in the model phytopathogen Magnaporthe oryzae. Ech1 was found to be essential for conidial germination and viability of older hyphae. Unlike wild-type Magnaporthe, the ech1D failed to utilize C14 fatty acid and was partially impeded in growth on C16 and C18 fatty acids. Surprisingly, loss of b-oxidation led to significantly altered mitochondrial morphology and integrity with ech1D showing predominantly vesicular/ punctate mitochondria in contrast to the fused tubular network in wild-type Magnaporthe. The ech1D appressoria were aberrant and displayed reduced melanization. Importantly, we show that the significantly reduced ability of ech1D to penetrate the host and establish therein is a direct consequence of enhanced sensitivity of the mutant to oxidative stress, as the defects could be remarkably reversed through exogenous antioxidants. Overall, our comparative analyses reveal that peroxisomal lipid catabolism is essential for appressorial function of host penetration, whereas mitochondrial b-oxidation primarily contributes to conidial viability and maintenance of redox homeostasis during host colonization by Magnaporthe.

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“…Selected structures can be visualized from various angles, yielding detailed information about association and arrangement of organelles. For comparative reasons and to minimize misinterpretation due to fixation artifacts, both chemical fixation and cryofixation of yeast cells Konomi et al, 2003;Feron et al, 2005;Winey et al, 2005;Binns et al, 2006) were used in this study to obtain detailed data about organelle organization and membrane association in yeast.…”

Section: Introductionmentioning

confidence: 99%

Organelle association visualized by three-dimensional ultrastructural imaging of the yeast cell

Perktold¹,

Zechmann²,

Daum³

et al. 2007

FEMS Yeast Research

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This study was aimed at a better understanding of organelle organization in the yeast Saccharomyces cerevisiae with special emphasis on the interaction and physical association of organelles. For this purpose, a computer aided method was employed to generate three-dimensional ultrastructural reconstructions of chemically and cryofixed yeast cells. This approach showed at a high level of resolution that yeast cells were densely packed with organelles that had a strong tendency to associate at a distance of <30 nm. The methods employed here also allowed us to measure the total surface area and volume of organelles, the number of associations between organelles, and the ratio of associations between organelles per surface area. In general, the degree of organelle associations was found to be much higher in chemically fixed cells than in cryofixed cells, with endoplasmic reticulum/plasma membrane, endoplasmic reticulum/mitochondria and lipid particles/nuclei being the most prominent pairs of associated fractions. In cryofixed cells, similar preferences for organelle association were seen, although at lower frequency. The occurrence of specific organelle associations is believed to be important for intracellular translocation and communication. Membrane contact as a possible means of interorganelle transport of cellular components, especially of lipids, is discussed.

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Metabolism of fatty acid in yeast: Characterisation of Î²-oxidation and ultrastructural changes in the genusSporidiobolussp. cultivated on ricinoleic acid methyl ester

Cited by 18 publications

References 30 publications

Effects of metal ions on growth, β-oxidation system, and thioesterase activity of Lactococcus lactis

Effects of metal ions on growth, β-oxidation system, and thioesterase activity of Lactococcus lactis

Mitochondrial β‐oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae

Organelle association visualized by three-dimensional ultrastructural imaging of the yeast cell

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