Metabolism of dihydroxyacetone in rat liver preparations

Tsao, Makepeace U.; Teagan, Susan J.; Borondy, Paul E.; Hogg, James F.

doi:10.1016/0026-0495(65)90065-x

Cited by 7 publications

(3 citation statements)

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“…While the precise mechanism of DHAc transport is not known, its entry into the hepatic Embden-Meyerhof pathway is rapid as evidenced by the downstream metabolites observed. DHAc kinase produces DHAP from DHAc, and has an activity of 0.71 units/mg protein in the rat liver 18 . Similar activities for the kinase are expected in the kidney 19 .…”

Section: Discussionmentioning

confidence: 99%

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐¹³C]dihydroxyacetone

Marco‐Rius

Morze

Sriram

et al. 2016

Magnetic Resonance in Med

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Purpose To investigate acute changes in glucose metabolism in liver and kidneys in vivo after a bolus injection of either fructose or glucose, using hyperpolarized [2‐13C]dihydroxyacetone. Methods Spatially registered, dynamic, multi-slice MRS were acquired for the metabolic products of [2-13C]dihydroxyacetone in liver and kidneys. Metabolism was probed in 13 fasted rats at three time points: 0, 70 and 140 minutes. At 60 minutes, rats were injected intravenously with fructose (n=5) or glucose (n=4) at 0.8 g/kg to initiate acute response. Controls (n=4) did not receive a carbohydrate challenge. Results Ten minutes after fructose infusion, levels of [2-13C]phosphoenolpyruvate and [2-13C]glycerol-3-phosphate halved in liver: 51% (P=0.0010) and 47% (P=0.0001) of baseline, respectively. Seventy minutes later, levels returned to baseline. The glucose challenge did not alter the signals significantly, nor did repeated administration of the dihydroxyacetone imaging bolus. In kidneys, no statistically significant changes were detected after sugar infusion other than a 20% increase of the glycerol-3-phosphate signal between 10 and 80 minutes after fructose injection (P=0.0028). Conclusion Hyperpolarized [2-13C]dihydroxyacetone detects a real-time, transient metabolic response of the liver to an acute fructose challenge. Observed effects possibly include ATP depletion and changes in the unlabeled pool sizes of glycolytic intermediates.

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Section: Discussionmentioning

confidence: 99%

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐¹³C]dihydroxyacetone

Marco‐Rius

Morze

Sriram

et al. 2016

Magnetic Resonance in Med

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show abstract

“…muZatta (2,3). Aside from study of human blood groups, much of the early work was concerned with study of the antigenic structure of erythrocytes of Old World monkeys, particularly the species Macaca mulatta (rhesus monkey).…”

Section: Oregon Regional Primate Research Center Beavertonmentioning

confidence: 99%

“…has been shown to be insulin dependent (3,4). This enzyme, too, is absent from the liver of the alloxan diabetic rat.…”

mentioning

confidence: 99%

Effect of Chlorothiazide on Rat Liver Glucokinase, Hexokinase and Dihydroxyacetone-kinase Activities.

Borondy

Weller

1965

Experimental Biology and Medicine

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Reductive metabolism of D-glyceraldehyde, L-glyceraldehyde and dihydroxyacetone in rat liver

Feraudi

Schmolz

1976

International Journal of Biochemistry

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Metabolism of dihydroxyacetone in rat liver preparations

Cited by 7 publications

References 10 publications

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐¹³C]dihydroxyacetone

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐¹³C]dihydroxyacetone

Effect of Chlorothiazide on Rat Liver Glucokinase, Hexokinase and Dihydroxyacetone-kinase Activities.

Reductive metabolism of D-glyceraldehyde, L-glyceraldehyde and dihydroxyacetone in rat liver

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Metabolism of dihydroxyacetone in rat liver preparations

Cited by 7 publications

References 10 publications

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐13C]dihydroxyacetone

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐13C]dihydroxyacetone

Effect of Chlorothiazide on Rat Liver Glucokinase, Hexokinase and Dihydroxyacetone-kinase Activities.

Reductive metabolism of D-glyceraldehyde, L-glyceraldehyde and dihydroxyacetone in rat liver

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Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐¹³C]dihydroxyacetone

Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐¹³C]dihydroxyacetone