Metabolism of DHEA in postmenopausal women following percutaneous administration

Labrie, Fernand; Bélanger, Alain; Bélanger, Patrick; Bérubé, René; Martel, Céline; Cusan, Lionel; Gómez, José L.; Candas, Bernard; Chaussade, V.; Castiel, Isabelle; Deloche, Claire; Leclaire, Jacques

doi:10.1016/j.jsbmb.2006.09.034

Cited by 115 publications

(121 citation statements)

References 28 publications

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“…GC-MS requires solvolysis prior to E 1 assay, whereas LC-MS/MS [19] is more practicable and RIA after chromatographic purification requires a less expensive apparatus, but is cumbersome. According to the material available, these methods could allow accurate plasma E 1 S level determination, which is very important and useful in oncology and endocrinology [12,25] since it is a precursor of unconjugated estrogens.…”

Section: Resultsmentioning

confidence: 99%

“…This condition was maintained for 2 min, and then the column was flushed with 95 % B for 2 min and re-equilibrated to initial conditions for an additional 2 min. transitions for E 1 S and E 1 S-d 4 , respectively [19].…”

Section: Lc-ms/ms Assay (Methods I)mentioning

confidence: 99%

“…Briefly, as previously published [19], plasma samples (100 µL) were diluted in PBS (2 mL) containing a deuterated internal E 1 S standard, then solid-phase extracted using Oasis HLB SPE columns. E 1 S analytes were eluted in 4 mL of methanol then evaporated at 35°C and reconstituted in 125 µL of methanol:water (50:50, v:v).…”

Section: Lc-ms/ms Assay (Methods I)mentioning

confidence: 99%

“…In an attempt to replace this specific but cumbersome RIA assay method, we tried a direct convenient E 1 S kit DSL-5400. In the present study, this direct E 1 S RIA method was compared with previously described E 1 S RIA [13] and with mass spectrometry coupled either with gas chromatography (GC-MS) or liquid chromatography (LC-MS/MS) in plasma samples from men [19].…”

Section: Introductionmentioning

confidence: 99%

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Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Giton¹,

Caron²,

Bérubé³

et al. 2010

Clinica Chimica Acta

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, et al.. Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS).: Plasma estrone sulfate assay in men: comparison between four different assay methods. Clinica Chimica Acta, Elsevier, 2010, 411 (17-18) Acknowledgments:The authors thank Dr. Noah Hardy for editing the manuscript. Methods:We simultaneously assayed estrone sulfate in the plasma of one hundred men aged 30-50 years, according to LC-MS/MS, GC-MS after solvolysis of E 1 S, radioimmunoassay after a chromatographic purification step, and a direct RIA commercial kit.Results: Estrone sulfate plasma levels obtained with the first three methods were not significantly different. However, estrone sulfate levels measured by the direct RIA were threefold higher than those obtained by the first three methods. We showed that the excessively high estrone sulfate levels obtained with the direct RIA kit had two origins:interference by high dehydroepiandrosterone sulfate plasma levels in men, and estrone sulfate inaccurate low concentrations in the standards. Conclusion:The LC-MS/MS method can be considered as an optimum option for clinical laboratory. The GC-MS method requires solvolysis to estrone, but allows simultaneous unconjugated steroid measurement. RIA method, with chromatographic purification, is cumbersome, but less expensive. DSL-5400 kit yielded estrone sulfate plasma levels that were too high.Plasma estrone sulfate assay in men: comparison between four different assay methods 3

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Section: Resultsmentioning

confidence: 99%

Section: Lc-ms/ms Assay (Methods I)mentioning

confidence: 99%

Section: Lc-ms/ms Assay (Methods I)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Giton¹,

Caron²,

Bérubé³

et al. 2010

Clinica Chimica Acta

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“…LH, FSH, and SHBG were measured as described previously (3,7). T and E 2 were measured using gas chromatography-mass spectrometry as described before (11,12). The lower limit of quantification of T was 170 pmol/liter, and of E 2 , 2.2 pmol /liter.…”

Section: Hormone Assaysmentioning

confidence: 99%

Effect of Polymorphisms in Selected Genes Involved in Pituitary-Testicular Function on Reproductive Hormones and Phenotype in Aging Men

Huhtaniemi

Pye

Holliday

et al. 2010

The Journal of Clinical Endocrinology & Metabolism

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, and the European Male Aging Study Group* † Context: Polymorphisms in genes involved in regulation, biosynthesis, metabolism, and actions of testicular sex hormones may influence hormone balance and phenotype of aging men. Objective:We investigated the relationships between polymorphisms in genes related to pituitarytesticular endocrine function and health status. Design and Setting:Using cross-sectional baseline data, we conducted a multinational prospective cohort observational study consisting of a population survey of community-dwelling men.Participants: A total of 2748 men, aged 40 -79 (mean Ϯ SD, 60.2 ϩ 11.2) yr, were randomly recruited from eight European centers. Forty-three polymorphisms were genotyped in the following genes: androgen receptor (AR), estrogen receptor-␣ and -␤ (ESR1 and ESR2), steroid 5␣-reductase type II (SRD5A2), 17␣-hydroxylase/17,20-lyase (CYP17A1), aromatase (CYP19A1), sex hormone-binding globulin (SHBG), LH ␤-subunit (LHB), and LH receptor (LHCGR). Main Outcome Measures:We measured the associations between gene polymorphisms and endocrine, metabolic, and phenotypic parameters related to aging and sex hormone action. Results: Several polymorphisms in SHBG, ESR2, AR, CYP19A1, and LHB were significantly associated with circulating levels of SHBG, LH, total, free, and bioavailable testosterone and estradiol, the LH ϫ testosterone product, and indices of insulin sensitivity. Apart from several previously reported associations between genes affecting estrogen levels and heel ultrasound parameters, no associations existed between polymorphisms and nonhormonal variables (anthropometry, blood lipids, blood pressure, hemoglobin, prostate symptoms, prostate-specific antigen, sexual dysfunction, cognition). Conclusion:In aging men, polymorphisms in genes related to the pituitary-testicular endocrine function significantly influence circulating LH, testosterone, and estradiol levels, but the downstream effects may be too small to influence secondary phenotypic parameters. (J Clin Endocrinol Metab 95:

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Dehydroepiandrosterone for women in the peri- or postmenopausal phase

Scheffers

Armstrong

Cantineau

et al. 2015

Cochrane Database of Systematic Reviews

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Metabolism of DHEA in postmenopausal women following percutaneous administration

Cited by 115 publications

References 28 publications

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Effect of Polymorphisms in Selected Genes Involved in Pituitary-Testicular Function on Reproductive Hormones and Phenotype in Aging Men

Dehydroepiandrosterone for women in the peri- or postmenopausal phase

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