Metabolism of Dehydroisoandrosterone and Other Steroids by Surviving Slices of the Potato Tuber and Additional Plant Tissues

Abstract. 13-Glucuronidase from callus cultures of Scutellaria baicalensis Georgi was purified to apparent homogeneity by fractionated ammonium-sulfate precipitation and chromatography on diethylaminoethyl-cellulose, hydroxylapatite and baicalin-conjugated Sepharose 6B. A 650-fold purification was obtained by this purification system. When subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis the purified protein migrated as a single band with a molecular mass of 55 kDa. We determined that the native enzyme has a molecular mass of 230 kDa using gel-filtration chromatography. These results suggested that the enzyme exists as a homotetramer composed of four identical 55-kDa subunits. The enzyme showed a broad pH optimum between 7.0 and 8.

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Metabolism of Dehydroisoandrosterone and Other Steroids by Surviving Slices of the Potato Tuber and Additional Plant Tissues

Cited by 17 publications

References 5 publications

Biochemistry of the Multiple Forms of Glycosidases in Plants

Biochemistry of the Multiple Forms of Glycosidases in Plants

Secondary Plant Substances

Purification and characterization of flavone-specific ?-glucuronidase from callus cultures of Scutellaria baicalensis Georgi

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