Metabolism of DD-2,6-Diaminopimelic Acid by a Diaminopimelate-requiring Mutant of Bacillus megaterium

Saleh, Farangis; White, Peter J

doi:10.1099/00221287-115-1-95

Cited by 12 publications

(4 citation statements)

References 8 publications

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“…This organism is a second stage mutant derived from strain SW 1 (parent strain 8. megaterium, NCIB 7581). Mutant SW1 requires A,pm for growth and has been shown to lack the enzyme N-acetyl-LLdiaminopimelate deacylase (Saleh & White 1979). In addition to this enzyme, GW1 also lacks diaminopimelic acid decarboxylase and therefore requires A,pm and lysine to be supplied for growth (White, personal communication).…”

Section: Bacillus Megaterium Gw1mentioning

confidence: 99%

The in vitro metabolism of free and bacterially‐bound 2,2'‐diaminopimelic acid by rumen micro‐organisms

Masson

Ling

1986

Journal of Applied Bacteriology

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Protozoa and bacteria were isolated from the rumen of a sheep given a concentrate and hay diet and were incubated separately with either free 2,2'‐diaminopimelic acid (A2pm) as [G‐3H]A2pm or bacterially‐bound A2pm in the form of [G‐3H]A2pm‐labelled Bacillus megaterium GW1. Lysine was the only radiolabelled metabolite produced in pellet and supernatant fluid when ciliates were incubated with 0·1 mmol/l free [G‐3H]A2pm; pipecolate was an additional product when 1·0 and 8·0 mmol/l A2pm were used. As well as incorporating A2pm, rumen bacteria decarboxylated it and produced a further three unidentified metabolites in the supernatant fluids. Protozoa rapidly engulfed and digested [G‐3H]A2pm‐labelled B. megaterium GW1. Radioactive A2pm and lysine were present in the protozoal pellets. A2pm, lysine and three other radiolabelled compounds were excreted into the supernatant fluids; the major product, designated G, appeared to be a series of A2pm‐containing peptides and derivatives. When rumen bacterial cells were incubated with [G‐3H]A2pm‐labelled B. megaterium GW1 they accumulated A2pm and lysine. Only A2pm and G were detected in bacterial supernatant fluids. The significance of these results to the use of A2pm as a marker of bacterial outflow from the rumen is assessed.

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Section: Bacillus Megaterium Gw1mentioning

confidence: 99%

The in vitro metabolism of free and bacterially‐bound 2,2'‐diaminopimelic acid by rumen micro‐organisms

Masson

Ling

1986

Journal of Applied Bacteriology

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“…The fractions have been designated as follows (Park & Hancock, 1960): cold TCA-soluble, pool metabolites fraction ; ethanol-soluble, lipid fraction ; hot TCA-soluble, nucleic acidteichoic acid-capsular polysaccharide fraction ; trypsin-soluble, protein fraction ; trypsin-insoluble, peptidoglycan fraction. To determine uptake of radioactivity, duplicate 0-1 ml samples of uninoculated growth medium, spent growth medium and resuspended cells were counted (Saleh & White, 1979). After each extraction in the cell fractionation scheme, the pellets were resuspended in 5 ml H 2 0 , and duplicate 0.1 ml samples were counted, except for the final peptidoglycan pellet which was resuspended directly in scintillation cocktail.…”

Section: Methodsmentioning

confidence: 99%

Survey of Taurine Uptake and Metabolism in Staphylococcus aureus

Smiley

Wilkinson

1983

Microbiology

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Taurine has been reported to be a component of the capsular polysaccharide of the encapsulated M strain of Staphylococcus aureus. This led to a study of the uptake and metabolism of [ 1,2-4C]taurine in a variety of encapsulated and unencapsulated S. aureus strains. Taurine was taken up by all strains studied. A discrepancy between uptake measured as depletion of radioactivity from growth medium and as cell-associated radioactivity suggested that taurine may be catabolized to CO, in some strains. In most strains, cell-associated radioactivity was located mainly in cold TCA-soluble (pool metabolites) fractions. About 90% of the cellassociated radioactivity was present in the pool metabolites fraction in the M strain, and about 10 % in hot TCA-soluble (nucleic acid-teichoic acid-capsular polysaccharide) fraction.Radioactivity in spent medium and the capsular polysaccharide-containing fraction appeared to be present as taurine in this strain. Radioactivity in the pool metabolites fraction of three of the strains examined did not chromatograph as taurine, indicating that taurine was converted into other cell metabolites. One strain incorporated radioactivity from taurine into cellular macromolecules, thus revealing a heterogeneity of staphylococcal taurine metabolism.

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“…1). NAD-DAC was first identified from studies involving the isolation of a B. megaterium DAP auxotroph (Saleh & White., 1979;Sundharadas & Gilvarg., 1967). The mutant strain possesses a non-functional form of NADwww.intechopen.com…”

Section: N-acetyldiaminopimelate Deacetylasementioning

confidence: 99%