Metabolism of Chylomicron Proteins in the Dog

Rodbell, Martin; Fredrickson, Donald S.; Ono, Katsuto

doi:10.1016/s0021-9258(18)70246-9

Cited by 121 publications

(3 citation statements)

References 11 publications

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“…The small amounts of aspartic acid and glutamic acid have been previously considered to be derived from the protein of overlapping chylomicrons and S; 0-10 lipoproteins, respectively. The reports by Rodbell (1958) and Rodbell and Fredrickson (1959) that N-terminal glutamic acid was not present in chylomicrons or lipoproteins of Sf >100, and by Grundy et al (1961) that only the high-density fraction of Sr 20-400 lipoproteins from nonlipemic plasma contained an ether-extracted residue similar to that found in 5/ 0-20 lipoproteins, provide a basis for this proposal. The results of the present study indicate that this hypothesis must be modified to account for a different protein distribution in Sf 10-400 lipoproteins from lipemic plasma.…”

Section: Discussionmentioning

confidence: 88%

Proteins of S_f 10-400 Lipoproteins from Lipemic Human Plasma^*

Bobbitt¹,

Levy²

1965

Biochemistry

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The relationship of the St10-400 lipoproteins to chylomicrons and other low-density lipoproteins in human lipemic plasma was investigated by comparing the protein portion of the lipoproteins. The Sf 10-400 lipoprotein class was divided into five fractions, each with a different lipid composition and encompassing a different Sf range. N-Terminal amino acid analyses of these fractions showed little variation in their protein composition. Serine and threonine were the major Nterminals and aspartic and glutamic acids the minor N-terminals in all fractions. The same four amino acids also were found, but in different ratios, in the chylomicron and Sf 0-10 classes. The 5 f 10-400 lipoproteins from different plasmas were exhaustively extracted with T A he four major classes of lipoproteins in human plasma have different chemical and physical properties.Although each class is usually defined in terms of a specific range of densities or a corresponding range of flotation rates, the transition from the properties of one density class to those of another is gradual and continuous (Lindgren and Nichols, 1960;Searcy and Bergquist, 1962). Such a continuous spectrum of different lipoproteins suggests either that the classes are transformed from one into another in the plasma, or that the classes are unrelated but occupy overlapping density ranges. A knowledge of the extent to which these two possibilities occur is necessary for understanding the metabolic interrelationships, if any, that exist between the various lipoproteins.Characterization of the protein components of various lipoproteins is a useful method of identifying different lipoproteins. The evidence indicates that a small number of different proteins serves as structural elements for the

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Section: Discussionmentioning

confidence: 88%

Proteins of S_f 10-400 Lipoproteins from Lipemic Human Plasma^*

Bobbitt¹,

Levy²

1965

Biochemistry

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“…Paper electrophoresis and N-terminal studies led to the conclusion that three different proteins constitute the protein moiety from chylomicrons (Rodbell and Fredrickson, 1959). One of these proteins was found to be identical with apo-HDL:1 another, apo-LP-B, was identified in chylomicrons using immunochemical methods (Lees, 1967).…”

mentioning

confidence: 99%

Characterization and quantitation of the apolipoproteins from human chyle chylomicrons

Kostner¹,

Holasek²

1972

Biochemistry

128

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“…Peptide Maps. Tryptic digests of the subunits were prepared according to Rodbell and Fredrickson (1959), except that the reaction was carried out for 6 hr at 25°. The resulting peptides were desalted, then separated in two dimensions on paper, according to Helinski and Yanofsky (1962).…”

Section: Methodsmentioning

confidence: 99%

Aspartate Transcarbamylase. Amino-Terminal Analyses and Peptide Maps of the Subunits^*

Hervé¹,

Stark²

1967

Biochemistry

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suggests that there are four each of the two different kinds of peptide chains in the native enzyme (molecular weight 3.1 X 10s). Taken together with the data of Changeux et al. (Changeux, J.-P., Gerhart, J. C., and Schachman, . K. (1967), in Regulation of Nucleic Acid and Protein Biosynthesis, Koningsberger, V. V., and Bosch, L, Ed., New York, N. Y., Elsevier, p 344) our results indicate that each of the binding sites for succinate (a competitive inhibitor of aspartatate) and cytidine triphosphate resides on a separate chain.

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Metabolism of Chylomicron Proteins in the Dog

Cited by 121 publications

References 11 publications

Proteins of S_f 10-400 Lipoproteins from Lipemic Human Plasma^*

Proteins of S_f 10-400 Lipoproteins from Lipemic Human Plasma^*

Characterization and quantitation of the apolipoproteins from human chyle chylomicrons

Aspartate Transcarbamylase. Amino-Terminal Analyses and Peptide Maps of the Subunits^*

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Metabolism of Chylomicron Proteins in the Dog

Cited by 121 publications

References 11 publications

Proteins of Sf 10-400 Lipoproteins from Lipemic Human Plasma*

Proteins of Sf 10-400 Lipoproteins from Lipemic Human Plasma*

Characterization and quantitation of the apolipoproteins from human chyle chylomicrons

Aspartate Transcarbamylase. Amino-Terminal Analyses and Peptide Maps of the Subunits*

Contact Info

Product

Resources

About

Proteins of S_f 10-400 Lipoproteins from Lipemic Human Plasma^*

Proteins of S_f 10-400 Lipoproteins from Lipemic Human Plasma^*

Aspartate Transcarbamylase. Amino-Terminal Analyses and Peptide Maps of the Subunits^*