Metabolism of Benzoic Acids and Phenols in Cell Suspension Cultures of Soybean and Mung Bean

Barz, Wolfgang; Schlepphorst, Rita; Wilhelm, Peter; Kratzl, K.; Tengler, Erich

doi:10.1515/znc-1978-5-610

Cited by 13 publications

(10 citation statements)

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“…In this report, the metabolism of SA to glucose conjugates and the vacuolar localization of these glucose conjugates are described. In contrast to a previous report that indicated that [ 14 C]SA supplied to soybean cells was converted only to SGE (Barz et al 1978), we have been able to demonstrate that SAG is the major metabolite of SA formed in soybean along with lower levels of glucosylated 2,5‐DHBA and MeSA 2‐ O ‐β‐ d ‐glucose. In addition, we have provided the first direct evidence that the glucose conjugates of SA are localized in the vacuole and that the SAGT activity that forms SAG is located outside the vacuole.…”

Section: Introductioncontrasting

confidence: 99%

“…The majority of the SA found in untreated and tobacco mosaic virus (TMV)‐inoculated leaves of tobacco ( Nicotiana tabacum L. ‘Xanthi‐nc’ NN genotype) was thought to exist primarily as SAG (Enyedi et al 1992, Malamy et al 1992); however, subsequent research revealed that small amounts of SGE could also be formed (Edwards 1994, Lee and Raskin 1998). In soybean cell suspension cultures, SA was reported to be exclusively metabolized to SGE (Barz et al 1978) and in a variety of other plant species SA was metabolized primarily to the 5‐ O ‐glucoside of gentisic acid (Cooper‐Driver et al 1972, Schulz et al 1993).…”

Section: Introductionmentioning

confidence: 99%

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Formation and vacuolar localization of salicylic acid glucose conjugates in soybean cell suspension cultures

Dean

Shah

Mohammed

2003

Physiologia Plantarum

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The metabolism and intracellular localization of salicylic acid (SA) was investigated in soybean (Glycine max[L.] cv Williams 82) cell suspension cultures. [7–14C]SA was added to the cell cultures, the metabolites were extracted from the cells at various time points and analysed by TLC and HPLC. The [7–14C]SA was taken up rapidly from the culture media and converted primarily to SA 2‐O‐β‐d‐glucose (SAG). Lower levels of glucosylated 2,5‐dihydroxbenzoic acid (gentisic acid) and methyl salicylate 2‐O‐β‐d‐glucose were also formed. Examination of the intracellular localization of the glucose conjugates revealed that all of the conjugates associated with the protoplasts were found in the vacuoles. An SA glucosyltransferase (SAGT) that could catalyse the formation of SAG from SA and UDP‐glucose could be extracted from soybean cells and assayed in vitro. Increasing concentrations of SA added to the culture media induced the SAGT activity. The highest levels of SAGT activity were observed in cells treated with 0.5 mM SA. The SAGT activity in these cells was 88‐fold greater than the SAGT activity in the untreated cells. The intracellular localization of the SAGT activity was also examined and it was determined that the majority of the SAGT activity in the protoplasts was located outside the vacuole. Therefore, it appears as if SAG is formed from SA outside the vacuole, presumably in the cytoplasm, and then subsequently transported into the vacuole where it accumulates.

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Section: Introductioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Formation and vacuolar localization of salicylic acid glucose conjugates in soybean cell suspension cultures

Dean

Shah

Mohammed

2003

Physiologia Plantarum

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“…The question arises as to why the inhibitory effect of SA on ethylene production only lasted for 5 h . One possibility is that SA is readily conjugated and/or degraded in rice leaf cells [2,7] . The present investigation demonstrated that SA inhibited the conversion of ACC to ethylene in rice leaves.…”

Section: Discussionmentioning

confidence: 99%

Salicylic acid inhibits the biosynthesis of ethylene in detached rice leaves

1993

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The effects of salicylic acid (SA) on ethylene biosynthesis in detached rice leaves were i nvestigated . S A at pH 3 .5 effectively inhibited ethylene production within 2 h of its application . It inhibited the conversion of ACC to ethylene, but did not affect the levels of ACC and conjugated ACC . Thus, the inhibitory effect of SA resulted from the inhibition of both synthesis of ACC and the conversion of ACC to ethylene .

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“…Their fate in the environment and their sensitivity to degradation systems in plants are determined by the nature of the substituents carried by their aromatic ring. Earlier studies have shown that plants, like microorganisms (Gottschalk, 1979), are able to degrade benzene by several pathways including oxidative cleavage of the aromatic ring and incorporation of the resulting products in the cell regular metabolism (Durmishidze and Ugrekhelidze, 1969;Barz et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

“…Ever since numerous literary data prove uptake and mineralization of benzene or its derivatives by higher plants (Ferro et al, 1997;Ugrekhelidze et al, 1997; see for review Korte et al, 2000). However, evidence for ring cleavage of aromatic compounds in plants is scarce.…”

Section: Introductionmentioning

confidence: 99%

Degradation of Aromatic Compounds in Plants Grown under Aseptic Conditions

Mithaishvili¹,

Scalla²,

Ugrekhelidze³

et al. 2005

Zeitschrift Für Naturforschung C

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The aim of the work is to investigate the ability of higher plants to absorb and detoxify environmental pollutants - aromatic compounds via aromatic ring cleavage. Transformation of 14C specifically labelled benzene derivatives, [1-6-14C]-nitrobenzene, [1-6-14C]-aniline, [1-14C]- and [7-14C]-benzoic acid, in axenic seedlings of maize (Zea mays L.), kidney bean (Phaseolus vulgaris L.), pea (Pisum sativum L.) and pumpkin (Cucurbita pepo L.) were studied. After penetration in plants, the above xenobiotics are transformed by oxidative or reductive reactions, conjugation with cell endogenous compounds, and binding to biopolymers. The initial stage of oxidative degradation consists in hydroxylation reactions. The aromatic ring can then be cleaved and degraded into organic acids of the Krebs cycle. Ring cleavage is accompanied by 14CO2 evolution. Aromatic ring cleavage in plants has thus been demonstrated for different xenobiotics carrying different substitutions on their benzene ring. Conjugation with low molecular peptides is the main pathway of aromatic xenobiotics detoxification. Peptide conjugates are formed both by the initial xenobiotics (except nitrobenzene) and by intermediate transformation products. The chemical nature of the radioactive fragment and the amino acid composition of peptides participating in conjugation were identified.

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Metabolism of Benzoic Acids and Phenols in Cell Suspension Cultures of Soybean and Mung Bean

Cited by 13 publications

References 0 publications

Formation and vacuolar localization of salicylic acid glucose conjugates in soybean cell suspension cultures

Formation and vacuolar localization of salicylic acid glucose conjugates in soybean cell suspension cultures

Salicylic acid inhibits the biosynthesis of ethylene in detached rice leaves

Degradation of Aromatic Compounds in Plants Grown under Aseptic Conditions

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