Metabolism of and inhibition by chlorobenzoates in Pseudomonas putida P111

Hernandez, B. S.; Higson, F K; Kondrat, R. W.; Focht, D. D.

doi:10.1128/aem.57.11.3361-3366.1991

Cited by 74 publications

(28 citation statements)

References 31 publications

(28 reference statements)

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“…The highperformance liquid chromatography analysis showed no 4-chlorophenol before the acidification (data not shown). The same kind of result was observed by Hernandez et al (12). So, 4-chlorophenol detected would not be a true intermediate metabolite of 4-chlorobiphenyl.…”

Section: Discussionsupporting

confidence: 82%

A Novel Transformation of Polychlorinated Biphenyls by Rhodococcus sp. Strain RHA1

Seto¹,

Kimbara²,

Shimura³

et al. 1995

Appl Environ Microbiol

232

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We have characterized a biphenyl degrader, Rhodococcus sp. strain RHA1. Biphenyl-grown cells of strain RHA1 efficiently transformed 45 components in the 62 major peaks of a polychlorinated biphenyl (PCB) mixture of Kanechlors 200, 300, 400, and 500 within 3 days, which includes mono-to octachlorobiphenyls. Among the intermediate metabolites of PCB transformation, di-and trichlorobenzoic acids were identified. The gradual decrease of these chlorobenzoic acids during incubation indicated that these chlorobenzoic acids would also be degraded by this strain. The effect of the position of chlorine substitution was determined by using PCB mixtures that have chlorine substitutions mainly at either the ortho or the meta position. This strain transformed both types of congeners, and strong PCB transformation activity of RHA1 was indicated. RHA1 accumulated 4-chlorobenzoic acid temporally during the transformation of 4-chlorobiphenyl. The release of most chloride in the course of 2,2-dichlorobiphenyl degradation was observed. These results suggested that RHA1 would break down at least some PCB congeners into smaller molecules to a considerable extent.

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Section: Discussionsupporting

confidence: 82%

A Novel Transformation of Polychlorinated Biphenyls by Rhodococcus sp. Strain RHA1

Seto¹,

Kimbara²,

Shimura³

et al. 1995

Appl Environ Microbiol

232

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“…Proposed pathways for aerobic degradation of fluorobenzoates and benzoate (adapted from [218]). 46 2,5-dichloro-and 2,3,5-trichiorobenzoate to chlorocatechols was also reported for a Pseudomonas putida strain [209].…”

Section: Aerobic Degradation Of Halobenzoatesmentioning

confidence: 63%

Microbial breakdown of halogenated aromatic pesticides and related compounds

Häggblom

1992

FEMS Microbiology Letters

363

132

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Considerable progress has been made in the last few years in understanding the mechanisms of microbial degradation of halogenated aromatic compounds. Much is already known about the degradation mechanisms under aerobic conditions, and metabolism under anaerobiosis has lately received increasing attention. The removal of the halogen substituent is a key step in degradation of halogenated aromatics. This may occur as an initial step via reductive, hydrolytic or oxygenolytic mechanisms, or after cleavage of the aromatic ring at a later stage of metabolism. In addition to degradation, several biotransformation reactions, such as methylation and polymerization, may take place and produce more toxic or recalcitrant metabolites. Studies with pure bacterial and fungal cultures have given detailed information on the biodegradation pathways of several halogenated aromatic compounds. Several of the key enzymes have been purified or studied in cell extracts, and there is an increasing understanding of the organization and regulation of the genes involved in haloaromatic degradation. This review will focus on the biodegradation and biotransformation pathways that have been established for halogenated phenols, phenoxyalkanoic acids, benzoic acids, benzenes, anilines and structurally related halogenated aromatic pesticides. There is a growing interest in developing microbiological methods for clean‐up of soil and water contaminated with halogenated aromatic compounds.

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“…Chlorobenzoatedegrading bacteria were shown frequently to be absent in soils while PCB-co-metabolizing strains were routinely isolated from contaminated soil by the enrichment on biphenyl (Hickey et al 1993). Hernandez et al (1991) isolated Pseudomonas putida P111 which was shown to have the greatest range of chlorobenzoate utilization yet reported. Further studies (Brenner et al 1993) revealed the presence of the gene for ortho-chlorobenzoate-1,2-dioxygenase (OCBD) on transmissible plasmid pPB111 which was shown to be responsible for the extraordinarily broad degradation activities of this strain.…”

Section: Introductionmentioning

confidence: 99%

Characterization of chlorobenzoate degraders isolated from polychlorinated biphenyl-contaminated soil and sediment in the Czech Republic

et al. 1999

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Two polychlorinated biphenyl‐contaminated sites in the Czech Republic, a soil at Zamberk and a sediment sludge at Milevsko, were screened for the presence of chlorobenzoate degraders. Sixteen different chlorobenzoate degraders were isolated from the soil compared with only three strains isolated from the sediment. From these strains, only four soil degraders and one strain isolated from the sediment, respectively, were shown to possess a complete chlorobenzoate (CB) pathway. Bacteria isolated from the soil have expressed more flexibility for CB degradation, namely in the case of ortho‐chlorinated benzoates. They all possessed large plasmids, the restriction patterns of which were compared. Plasmids in Pseudomonas sp. A7, A8, A18 and A19, respectively, were cured and found to encode at least part of the metabolic pathway involved in the growth on ortho‐chlorinated benzoates.

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Metabolism of and inhibition by chlorobenzoates in Pseudomonas putida P111

Abstract: Pseudomonas putida Pill was isolated by enrichment culture on 2,5-dichlorobenzoate and was also able to grow on 2-chloro-, 3-chloro-, 4-chloro-, 2,3-dichloro-, 2,4-dichloro-, and 2,3,5-trichlorobenzoates. However, * Corresponding author.

Cited by 74 publications

References 31 publications

A Novel Transformation of Polychlorinated Biphenyls by Rhodococcus sp. Strain RHA1

A Novel Transformation of Polychlorinated Biphenyls by Rhodococcus sp. Strain RHA1

Microbial breakdown of halogenated aromatic pesticides and related compounds

Characterization of chlorobenzoate degraders isolated from polychlorinated biphenyl-contaminated soil and sediment in the Czech Republic

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