Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolites

Multi-laboratory study of the analysis and kinetics of stanozolol and its metabolites in treated calves†

“…1). [5][6][7] Other workers have used these metabolites in an effort to improve the GC-MS 8,9 or LC-MS-MS 10 methodology for the determination of these compounds in doping control. For veterinary inspection, it was demonstrated by GC-MS and LC-MS that 16-hydroxystanozolol (16-OHStan) 11,12 and 4,16-dihydroxystanozolol (4,16-diOHStan) 12 were the major metabolites of Stan in cattle.…”

Determinação de esteróides androgênicos anabólicos em urina por cromatografia gasosa acoplada à espectrometria de massas

“…meio alcalino (Schanzer, Donike, 1993;Ayotte, Goudreault, Chanlebois, 1996;Özer, Temizer, 1997;Schanzer, 1998;Yoon, Lee, 2001;Marcos et al, 2002). Na extração em fase sólida, a resina XAD-2 e colunas C-18 são as mais utilizadas (Özer, Temizer, 1997;Yoon, Lee, 2001).…”

“…Na literatura científica alguns métodos cromatográ-ficos foram propostos para detecção de EAA em urina (Schanzer, Donike, 1993;Ayotte, Goudreault, Chanlebois, 1996;Özer, Temizer, 1997;Schanzer, 1998;Yoon, Lee, 2001;Marcos et al, 2002). No presente trabalho, um método analítico por GC-MS para a determinação de EAA e/ou seus produtos de biotransformação em amostras de urina foi validado de acordo com os guias internacionais e nacionais que norteiam as normas de boas práticas laboratoriais (World Anti-Doping Agency, 2003a;ANVISA, 2003).…”

Characterization and Quantification of Fluoxymesterone Metabolite in Horse Urine by Gas Chromatography/Mass Spectrometry

“…Based on these results, the metabolites were presumed to be a C-16 hydroxylated isomer; a 3-keto or 4-ene reduced and C-16 hydroxylated isomer; a 3-keto and 4-ene reduced and C-16 hydroxylated isomer; a C-6,16 dihydroxylated isomer; a 3-keto or 4-ene reduced and C-6,16 dihydroxylated isomer; and a 3-keto and 4-ene reduced and C-6,16 dihydroxylated isomer, and they have been reported as FOM metabolites in horse or humans. [3][4][5][6][7] However, their peaks in the total ion chromatograms were very small compared with those of the metabolites of the unconjugated fraction, particularly F1.…”

“…2 Schänzer et al identified FOM metabolites originating from 17-epimerization, 6b-hydroxylation, and 3-keto reduction, as well as 17,17-dimethyl-18-norandrost-13-ene, using synthesized reference compounds. [3][4][5][6] On the other hand, Stanley et al reported on FOM metabolites in horse urine; metabolites originating from C-6 or C-6, C-16 hydroxylation, and the 17-epimer were confirmed by LC/MS and GC/MS analyses. 7 To develop a suitable doping analysis of FOM in our laboratory, we investigated FOM metabolites in horse urine, and detected metabolites that have not been reported to date.…”