Metabolism of ?-aminoisobutyric acid in mungbean hypocotyls in relation to metabolism of 1-aminocyclopropane-1-carboxylic acid

Liu, Yu; Su, Ling-Yuan; Yang, Shang Fa

doi:10.1007/bf00394575

Cited by 20 publications

(6 citation statements)

References 18 publications

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“…Studies of the reaction products are important to the understanding of the chemical mechanism by which ACCO catalyzes its reaction. Previous studies showed that radiolabeled R-AIB was metabolized in mung bean hypocotyls to CO 2 and that this reaction was inhibited by the addition of ACC, suggesting that the (then unidentified) enzyme responsible for ethylene production from ACC was responsible for R-AIB turnover (20). It was assumed that R-AIB was oxidized at the amino group in a manner similar to that of ACC and degraded to CO 2 and acetone.…”

Section: Steady-state Kinetics Of O 2 Consumption With Substratementioning

confidence: 99%

“…Since CO 2 was detected as a product of AIB metabolism in mung bean hypocotyls (20), we determined the ability of ACCO to turn over ACC or the acyclic substrate analogues (R-AIB, D-Ala, and Gly) by CO 2 formation. [1-14 C]ACC, [1-14 C]-D-Ala, [1-14 C]-R-AIB, or [1-14 C]Gly was incubated with the ACCO-Fe(II) complex in a closed reaction vial containing a CO 2 trap (see Experimental Procedures).…”

Section: Steady-state Kinetics Of O 2 Consumption With Substratementioning

confidence: 99%

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Mechanistic Investigations of 1-Aminocyclopropane 1-Carboxylic Acid Oxidase with Alternate Cyclic and Acyclic Substrates

et al. 2006

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The behavior of three cyclic and three acyclic analogues of 1-aminocyclopropane-1-carboxylic acid (ACC) with ACC oxidase has been analyzed with regard to turnover rates, product distribution, and O(2) uncoupling. The cyclic analogues all form ethylene, and the acyclic analogues all undergo decarboxylation. The degree of uncoupling varies from almost none (ACC) to 21-fold (glycine), while turnover rates (k(cat)) are all within a factor of 4-fold of that of ACC. The aggregate data point toward a rate-determining formation of an activated iron-oxo intermediate, which partitions between amine oxidation and reductive uncoupling in a manner that is dependent on substrate structure.

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Section: Steady-state Kinetics Of O 2 Consumption With Substratementioning

confidence: 99%

Section: Steady-state Kinetics Of O 2 Consumption With Substratementioning

confidence: 99%

Mechanistic Investigations of 1-Aminocyclopropane 1-Carboxylic Acid Oxidase with Alternate Cyclic and Acyclic Substrates

et al. 2006

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“…AIB uptake in plant cells showed characteristics similar to ACC uptake, indicating that these amino acids may behave similarly in plants (Saftner and Baker, 1987). AIB is not converted to ethylene but may be converted to CO, and malonyl-AIB, but not to the extent to which ACC is usually metabolized to ethylene and malonyl-ACC (Liu et al, 1984). Since AIB is not converted to ethylene, these experiments were carried out in non-pretreated flowers.…”

Section: Treatment With Chemicalsmentioning

confidence: 90%

The Role of Ethylene in Interorgan Signaling during Flower Senescence

Woltering¹,

Somhorst²,

Veer³

1995

Plant Physiol.

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Also within flowers, ACC translocation has been implicated in interorgan C0"miCatiOn. SeqUential inCreaSeS in ACC concentrations in different flower parts following pollination or stigma wounding indicated ACC translocation (Nichols et al., 1983;Nichols and Frost, 1985). More direct evidente was obtained in experiments in which flowers were treated with radiolabeled ACC, In carnation, Reid et al, (1984) showed that petals produced radiolabeled ethylene following treatment of the stigma with radiolabeled ACC. Similarly, in ethYlene following aPPlication of r d i d~b e l e d ACC to the rostehm (modified lobe of the stigma) (Woltering, 1990a). These data suggest that ACC may act as a mobile senescence factor during senescence of flowers.In orchid flowers, senescence is rapidly induced by emasculation (removal of the anther cap and the pollinia) and by pollination. ~~l l~~i~~ these treatments, an increase in ethylene production and rapid perianth senescence occurs. In addition, in Cymbidium, red coloration of the labellum (lip) as a result of increased anthocyanin synthesis is apparent within 24 h of emasculation or pollination (Burg and Dijkman, 1967; Arditti et al., 1973;Woltering and Somhorst, 1990). These responses have been used to study interorgan relations during senescence, and numerous experiments have indicated that translocation of ACC from the central column to the other flower parts plays an important role in coordination of the senescence process (Woltering, 1990a(Woltering, , 1990bONeill et al., 1993).In addition, it was shown in Cymbidium flowers that application of ethylene to the central column induces peta1 senescence, indicating that ethylene can also be translocated and that this may represent an additional signaling mechanism (Woltering, 1990a). In Phalaenopsis, ONeill et al. (1993) also suggested a role for ethylene in interorgan signaling following pollination.In this paper we describe experiments that we used to examine the relative contributions of ACC and ethylene translocation in interorgan communication during senescence of flowers.

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“…Formation of N-heteroatom derivatives leads to the nitrene or nitrenium ion and results in a concerted mechanism, while electron transfer/free radical oxidants lead to a radical cation and result in a nonconcerted mechanism. Despite the significant evidence in favor of the radical pathway, reference to N-hydroxylation and nitrenium ion formation as a key step in ethylene biosynthesis has persisted, particularly in the plant physiology literature (2,(43)(44)(45)(46). The sequence similarity of the EFE and several hydroxylase enzymes (vide supra) has only added fuel to this fire.…”

Section: The Sequential Single-electron Transfer Mechanism It Has Sumentioning

confidence: 99%

Ethylene Biosynthesis from 1-Aminocyclopropanecarboxylic Acid

Pirrung

1993

Natural and Engineered Pest Management Agents

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Advances in the molecular biology of ethylene formation in plants have provided essential knowledge for the isolation and purification of the ethylene-forming enzyme from apple fruit.Comparisons with related enzymes have provided guidance concerning the mechanism of ethylene biosynthesis, and the history of mechanistic postulates for this process has been reviewed in this light. The biosynthesis of ethylene is of interest because of the role that it plays in catabolic proceses in plant physiology: ripening, senescence, abscission, germination, and response to environmental stress.Ethylene is an endogenous plant hormone that controls a number of processes in growth and development.Since the original report (1) that 1-aminocyclopropanecarboxylic acid (ACC) is the immediate precursor of ethylene (eq 1), the macromolecule that conducts the oxidative conversion of ACC to ethylene has been an important topic in biochemistry, and plant physiology. However, because over a decade of effort in protein purification had failed to provide authentic cell-free ethylene-forming enzyme (EFE) activity, the lore developed that the enzyme is membrane-bound and does not survive breakage of the cell wall (2). Consequently, the many mechanistic studies of ethylene production (3-8) have been limited to biosynthesis experiments in which only substrates and products can be inferred. Recent advances in molecular genetics, including the determination of the DNA sequence of the EFE gene, have provided information concerning the class of proteins to which EFE belongs but cannot address key issues concerning the mechanism. This chapter reviews the genetics and mechanisms of ethylene biosynthesis and connects them to our recent purification of the ethylene-forming enzyme.

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Metabolism of ?-aminoisobutyric acid in mungbean hypocotyls in relation to metabolism of 1-aminocyclopropane-1-carboxylic acid

Cited by 20 publications

References 18 publications

Mechanistic Investigations of 1-Aminocyclopropane 1-Carboxylic Acid Oxidase with Alternate Cyclic and Acyclic Substrates

Mechanistic Investigations of 1-Aminocyclopropane 1-Carboxylic Acid Oxidase with Alternate Cyclic and Acyclic Substrates

The Role of Ethylene in Interorgan Signaling during Flower Senescence

Ethylene Biosynthesis from 1-Aminocyclopropanecarboxylic Acid

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