Metabolism of adrenosterone☆

Metabolism of 11β-Hydroxyandrostenedione, Adrenosterone and Hydrocortisone to Urinary 11-Oxy 17-Ketosteroids1

“…The 5a/5/3 ratio ranged from 2.6 to 5.7, in good agreement with published studies. When 11KA served as the precursor, the ratio of 5a;/5/3 metabolites ranged from 2.9 to 8.6, confirming the findings of Bradlow et al (12). The patients with ovarian dysgenesis, Cushing's syndrome and virilizing adrenal tumor showed ratios of 2.5, 4.1 and 4.8, respectively.…”

“…The 5a/5/3 ratio of the C19O3 steroids of a single case of congenital adrenal hyperplasia given radioactive 110HA was 4.75 (4). Some years later, Slaunwhite et al (11) re-examined this problem and found 5a/5/3 ratios of 7 and 100 in the two cases studied; Bradlow et al (12) subsequently obtained a ratio of 4.6 in another study. However, the data in a case of Cushing's syndrome reported by Gold and Crigler (13) reveal that the 5a/5/3 ratio of the urinary Ci 9 O 3 steroids in this instance was only 0.1 (i.e., only 8.7% of these steroids were in the 5a configuration).…”

“…Bradlow et al (12) disagreed with these findings on the basis of two patients who received simultaneous injections of 11 OHA and 11KA. By contrast, they concluded that the 11-hydroxy and 11-keto groups were readily interconvertible, and did not have a differential effect on the 5a/50 ratio.…”

Induction of a deficiency of steroid delta 4-5 alpha-reductase activity in liver by a porphyrinogenic drug.

“…Phenobarbital is a prototype of druigs known to provoke htuman as well as experimental hepatic porphyria and to indtuce the cytochrome P-450-dependent mixed ftinction oxidase system in liver (1,2). This NADPH-requiring enzyme complex localized in the membranes of the endoplasmic retictultum mediates the oxidation of ntumerous, struictulrally diverse chemicals, incluiding natuLral steroid hormones (3 dione were prepared and administered and metabolites were isolated to radiochemical purity as described in oui previous studies (11,12,(18)(19)(20). Briefly, on dclay 1 of the study, an intravenous infusion of 5% glucose in water was begun and a weighed amount of radioactive testosterone was injected into the rubber tubing immediately adljacelnt to the needle and washed in with the intravenouis inf'usion over a 10-min period.…”

“…The 5,/-metabolites, 1 1-hydroxyetiocholanolone, and 1l-keto-etiocholanolone form only a small fraction of transformation products from 11-OHAD (usually less than 5-10%/c) (19,20). The latter compounds do not increase in conditions in which steroid A4-5a-reductase is depressed; instead a direct reduction of the C3 ketone to a C3-hydroxyl takes place and the double bond at C4-5 remains chemically intact (12,20). This pattern of The results of the metabolic studies with 1l-OHAD in the three normal subjects before and after phenobarbital are shown in Fig.…”