Metabolism of [8-<scp>L</scp>-[14C] phenylalanine]-angiotensin I in the pulmonary circulation

Ryan, James W.; Niemeyer, R.; Goodwin, Donald W.; Smith, Una

doi:10.1042/bj1250921

Cited by 29 publications

(10 citation statements)

References 7 publications

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“…In two experiments, effluent fractions were collected into test tubes (18 by 150 mm), each containing 1.0 ml of absolute ethanol. Radioactive peptides were purified and identified (2). Approximately 18 percent of the radioactivity was identified as angiotensin II, the remainder of the radioactivity being present 'as tetrapeptides (36 percent), tripeptides (22 percent), prolyl-phenylalanine (15 percent), and free phenylalanine (7 percent).…”

Section: Wolfgang Wiltschkomentioning

confidence: 99%

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Angiotensin I: Metabolism by Plasma Membrane of Lung

Ryan¹,

Smith²,

Niemeyer³

1972

Science

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(8-L-[(14)C] phenylalanine) angiotensin I is metabolized in one passage through blood-free lungs. Approximately 20 percent of the radioactivity emerges as angiotensin 11, the remainder as lower homologs. Radioactivity is not retained by the lungs but has the same volume of distribution and mean transit time as blue dextran, a compound unlikely to leave the intravascular space. Plasma membrane fractions of lung are capable of converting angiotensin I to angiotensin II. These data, taken together, indicate the circulating angiotensin I is metabolized by enzymes of the luminal surface of pulmonary endothelial cells.

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Section: Wolfgang Wiltschkomentioning

confidence: 99%

“…Reactions were stopped after 0.25, 5, 10, 15, 30, and 60 minutes of incubation by heating in a boiling water bath. Radioactive reaction products were identified by a combination of gel chromatography and paper electrophoresis (1,2).…”

Section: Wolfgang Wiltschkomentioning

confidence: 99%

Angiotensin I: Metabolism by Plasma Membrane of Lung

Ryan¹,

Smith²,

Niemeyer³

1972

Science

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“…Studying the metabolism of radioactive AI, Oparil ef d. IS] did not detect any products other than leucine or histidylleucine in vitro as well as in vim, and Ryan d al. [19] reported the presence of di-, tri-, and tetrapeptides, but did not find hexapeptide. We thus feel confident that what was measured as immunoreactive angiotensin I1 was actually the product of the action of converting enzyme upon angiotensin I and conclude from this study that effective extra-pulmonary conversion takes place during cardiopulmonary bypass in man.…”

Section: Diseiissionmentioning

confidence: 97%

“…The present study was designed to investigate the extrapulmonary conversion in vivo in man. The effect of pulmonary bypass upon tho concentrations of endogenous plasma angiotensin I and 11, determined by specific radioimmunoassays, and their relationship with the simultaneously measured plasma renin activity w; 19 examined during extra-corporeal circulation in 13 patienL9 undergoing major cardiac surgery.…”

mentioning

confidence: 99%

Relationship between Plasma Concentrations of Angiotensin I, Angiotensin II and Plasma Renin Activity during Cardio‐Pulmonary Bypass in Man*

Favre

Vallotton

Müller

1974

Eur J Clin Investigation

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Abstract. Several reports have demonstrated that the lungs are the most important site of conversion of angiotensin I to angiotensin II. The purpose of the present study was to assess the extent of extra‐pulmonary conversion hi man, during cardiopulmonary bypass. Plasma concentrations of angiotensin I and immunoreactive angiotensin II, and plasma renin activity were simultaneously determined, using specific radioimmunoassays, during extra‐corporeal circulation in 13 patients undergoing major cardiac surgery. Generally the renin‐angiotensin system was stimulated during cardiopulmonary bypass with maximum values occurring at different time. A highly significant correlation was found between plasma renin activity and angiotensin I and II concentrations respectively, as well as between these two peptides. Positive correlations were also obtained between arterial and venous samples for plasma renin activity and angiotensin I and II. Thus the presence of angiotensin II in plasma in the absence of pulmonary circulation and its parallel variations with plasma renin activity indicate that converting activity by extra‐pulmonary sources is not negligible.

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“…Obwohl schon im letzten Jahrhundert eine Filtrationsfunktion des Gefäßsystems erörtert wurde (1 1 1), konnte man erst in den letzten zwei Jahrzehnten feststellen, da13 das Lungenendothel eine entscheidende metabolische Funktion ausübt (1 14). So wurde nachgewiesen, daß vasoaktive Hormone wie Angiotensin I (85), Adeninnucleotide (86) und Amine (30) in der Lunge metabolisiert werden.…”

Section: Stellenwert Des Endothels Beim Ardsunclassified

Die Rolle des Endothels beim Adulten Respiratory Distress Syndrom (ARDS): Gegenwart und Zukunft

Kirkpatrick¹,

Mittermayer²

1989

Anästhesiol Intensivmed Notfallmed Schmerzther

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Metabolism of [8-L-[14C] phenylalanine]-angiotensin I in the pulmonary circulation

Cited by 29 publications

References 7 publications

Angiotensin I: Metabolism by Plasma Membrane of Lung

Angiotensin I: Metabolism by Plasma Membrane of Lung

Relationship between Plasma Concentrations of Angiotensin I, Angiotensin II and Plasma Renin Activity during Cardio‐Pulmonary Bypass in Man*

Die Rolle des Endothels beim Adulten Respiratory Distress Syndrom (ARDS): Gegenwart und Zukunft

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