Metabolism and Pathogenic Mechanisms of Parasitic Protozoa

Ultrastructural cytochemical identification of peroxisomes as organelles of terminal oxidation in amitochondrial trichomonad flagellates

“…( Figures 36C, D, E). The presence of mitochonrial-type succinic dehydro genase is questionable, however, in the light of the lack of biochemical evidence for this enzyme in trichomonads (See LITERATURE REVIEW) and the evidence for succinic acid as a principal metabolic end product excreted by these trichomonads (Shorb, 1964;Fulton, 1969). Alternatively, the increased level of formazan deposition over no-substrate control organisms ( Figures 35G, D) might be accounted for by metabolic efforts of the ceil to eliminate succinic acid leaking through the plasma membrane damaged by dimethylforamide, using endogenous glycogen stores as substrates.…”

Ultrastructural cytochemical identification of peroxisomes as organelles of terminal oxidation in amitochondrial trichomonad flagellates

“…Complete breakdown of the carbohydrates necessary for growth to CO^ and H^O via the TCA cycle does not occur (Shorb, 1964). Trichomonad metabolic end products, grown aerobically or anaerobically, are lactic, succinic, malic, acetic, and smaller amounts of other incompletely oxidized organic acids, Hg, CO^ and NH^ (Fulton, 1969). 2» vaginalis produces lactic acid (the glycolysis end product) and malic acid pre dominantly (Wellerson et al, 1960), while %.…”

“…Recent reviews of the biochemical studies of trichomonad metabolism include: Baernstein, 1963;Shorb, 1964;Danforth, 1967;Ryley, 1967;and Fulton, 1969. Trichomonad enzyme cytochemistry Trichomonad "mitochondriarelated" metabolism has also been partially characterized, and related to the structure of the organism, by light-microscope level enzyme cyto chemistry.…”

“…In Figures 32D and 34E ( Figures 36C, D, E). The presence of mitochonrial-type succinic dehydro genase is questionable, however, in the light of the lack of biochemical evidence for this enzyme in trichomonads (See LITERATURE REVIEW) and the evidence for succinic acid as a principal metabolic end product excreted by these trichomonads (Shorb, 1964;Fulton, 1969) Figures 39A and B). Also, tangential sections show depositions not appar ently associated with PAG-PGG, when the organelles are nearly out of the plane of section ( Figures 39C and D).…”

“…Lactate, like succinate, is a metabolic end product of trichomonad metabolism. (Shorb, 1964;Fulton, 1969). This substrate was used with moderate success by for localization of the peroxisomal flavoprotein, Q-hydroxy acid oxidase, in particulate sites at the light microscope level in rat kidney.…”