Metabolism and Metabolomics of Eukaryotes Living Under Extreme Conditions

Weber, Andreas P.M.; Horst, Robin J.; Barbier, Guillaume G.; Oesterhelt, Christine

doi:10.1016/s0074-7696(07)56001-8

Cited by 38 publications

(24 citation statements)

References 187 publications

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“…Our pyrosequencing study reveals a similarly high diversity of these organisms and even though it is assumed that most heterotrophic flagellates disappear above a salinity of 25 % (Pedrós-Alió 2004), this and other studies indicate that the diversity of these microorganisms in hypersaline habitats is strongly underestimated (Park et al 2003;Stock et al 2012). Our study and also previous studies Triadó-Margarit and Casamayor 2013;Heidelberg et al 2013) clearly question the traditional view that most extreme environments on Earth harbor a relatively low diversity of eukaryotic microorganisms, mostly restricted to few evolutionary lineages like some algae, heterotrophic flagellates and fungi (Weber et al 2007). …”

Section: Discussioncontrasting

confidence: 57%

Deep sequencing uncovers protistan plankton diversity in the Portuguese Ria Formosa solar saltern ponds

et al. 2014

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We used high-throughput sequencing to unravel the genetic diversity of protistan (including fungal) plankton in hypersaline ponds of the Ria Formosa solar saltern works in Portugal. From three ponds of different salinity (4, 12 and 38 %), we obtained ca. 105,000 amplicons (V4 region of the SSU rDNA). The genetic diversity we found was higher than what has been described from solar saltern ponds thus far by microscopy or molecular studies. The obtained operational taxonomic units (OTUs) could be assigned to 14 high-rank taxonomic groups and blasted to 120 eukaryotic families. The novelty of this genetic diversity was extremely high, with 27 % of all OTUs having a sequence divergence of more than 10 % to deposited sequences of described taxa. The highest degree of novelty was found at intermediate salinity of 12 % within the ciliates, which traditionally are considered as the best known and described taxon group within the kingdom Protista. Further substantial novelty was detected within the stramenopiles and the chlorophytes. Analyses of community structures suggest a transition boundary for protistan plankton between 4 and 12 % salinity, suggesting different haloadaptation strategies in individual evolutionary lineages as a result of environmental filtering. Our study makes evident the gaps in our knowledge not only of protistan and fungal plankton diversity in hypersaline environments, but also in their ecology and their strategies to cope with these environmental conditions. It substantiates that specific future research needs to fill these gaps.

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Section: Discussioncontrasting

confidence: 57%

Deep sequencing uncovers protistan plankton diversity in the Portuguese Ria Formosa solar saltern ponds

et al. 2014

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“…In fact, the pattern of catalysis for trx* approaches that of a eukaryotic thioredoxin (see Figure 1B). The combination of high stability and eukaryotic pattern of catalysis in trx* is a particularly suggestive one, as, in most cases, eukaryotic organisms are not thermophiles [27]. …”

Section: Resultsmentioning

confidence: 99%

Role of conservative mutations in protein multi-property adaptation

Rodríguez-Larrea

Pérez-Jiménez

Sánchez-Romero

et al. 2010

Biochemical Journal

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Protein physicochemical properties must undergo complex changes during evolution, as a response to modifications in the organism environment, the result of the proteins taking up new roles or because of the need to cope with the evolution of molecular interacting partners. Recent work has emphasized the role of stability and stability–function trade-offs in these protein adaptation processes. In the present study, on the other hand, we report that combinations of a few conservative, high-frequency-of-fixation mutations in the thioredoxin molecule lead to largely independent changes in both stability and the diversity of catalytic mechanisms, as revealed by single-molecule atomic force spectroscopy. Furthermore, the changes found are evolutionarily significant, as they combine typically hyperthermophilic stability enhancements with modulations in function that span the ranges defined by the quite different catalytic patterns of thioredoxins from bacterial and eukaryotic origin. These results suggest that evolutionary protein adaptation may use, in some cases at least, the potential of conservative mutations to originate a multiplicity of evolutionarily allowed mutational paths leading to a variety of protein modulation patterns. In addition the results support the feasibility of using evolutionary information to achieve protein multi-feature optimization, an important biotechnological goal.

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“…C. merolae is distinguished from others by the lack of a rigid cell wall, which enables its cells to be broken down simply by freeze-thawing. Galdieria is a facultative heterotroph, which contrasts to the other two groups Cyanidium and Cyanidioschyzon that are obligate autotrophy (Weber et al, 2007). Like most of other species of Cyanidiaceae (Weber et al, 2007), C. caldarium is moderately thermophilic (grows at temperatures around 42-45 ºC).…”

Section: Introductionmentioning

confidence: 99%

“…Galdieria is a facultative heterotroph, which contrasts to the other two groups Cyanidium and Cyanidioschyzon that are obligate autotrophy (Weber et al, 2007). Like most of other species of Cyanidiaceae (Weber et al, 2007), C. caldarium is moderately thermophilic (grows at temperatures around 42-45 ºC). This makes its proteins rather thermostable, which facilitates its use as a model organism for studies on photosynthetic pigment-protein complexes of red algae.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Acido-Tolerance and Characteristics of Photosystems in an Acidophilic and Thermophilic Red Alga, Cyanidium Caldarium

Enami

Adachi

Shen

2010

Cellular Origin, Life in Extreme Habitats and Astrobiology

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In this chapter, we describe the mechanisms of acido-tolerance in an acido-and thermo-philic red alga, Cyanidium caldarium. In spite of the extremely acidic environments it inhabits, the intracellular pH of Cyanidium cells is kept neutral by pumping out the protons previously leaked into the cells according to the steep pH gradient. The H + pump is driven by the plasma membrane ATPase, utilizing intracellular ATP produced by both oxidative phosphorylation and cyclic photophosphorylation via photosystem I. We also describe the characteristics and function of the two photosystems, Photosystem I (PSI) and II (PSII) in Cyanidium caldarium in comparison with those of cyanobacteria, other eukaryotic algae and higher plants, based on the crystal structures of the two complexes reported so far.

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Metabolism and Metabolomics of Eukaryotes Living Under Extreme Conditions

Cited by 38 publications

References 187 publications

Deep sequencing uncovers protistan plankton diversity in the Portuguese Ria Formosa solar saltern ponds

Deep sequencing uncovers protistan plankton diversity in the Portuguese Ria Formosa solar saltern ponds

Role of conservative mutations in protein multi-property adaptation

Mechanisms of Acido-Tolerance and Characteristics of Photosystems in an Acidophilic and Thermophilic Red Alga, Cyanidium Caldarium

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