Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans

Langberg, Henning; Skovgaard, Dorthe; Karamouzis, Michael; Bülow, Jens; Kjær, Michael

doi:10.1111/j.1469-7793.1999.919ab.x

Cited by 156 publications

(167 citation statements)

References 42 publications

(57 reference statements)

Supporting

Mentioning

152

Contrasting

Unclassified

Order By: Relevance

“…We choose to study IL‐6 as this inflammatory mediator has previously been linked to tendinopathy24 and COX‐2 as increased levels of COX‐2 have been shown in patellar tendinopathy,26, 27 loaded human tendon cells39 and injured tendons 40. Other markers of inflammation such as IL‐1 were not included in this study but have previously been implicated in tendinopathic tendons41, 42 or bursa of the shoulder 25.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Spiesz

Thorpe

Chaudhry

et al. 2015

Journal Orthopaedic Research

View full text Add to dashboard Cite

The role of inflammation in tendon injury is uncertain and a topic of current interest. In vitro studies of tendon accelerated overload damage can serve as a valuable source of information on the early stages of tendinopathy. Viable fascicle bundles from bovine flexor tendons were subjected to cyclic uniaxial loading from 1–10% strain. Immuno‐staining for inflammatory markers and matrix degradation markers was performed on the samples after mechanical testing. Loaded samples exhibited visible extracellular matrix damage, with disrupted collagen fibers and fiber kinks, and notable damage to the interfascicular matrix. Inflammatory markers COX‐2 and IL‐6 were only expressed in the cyclically loaded samples. Collagen degradation markers MMP‐1 and C1,2C were colocalized in many areas, with staining occurring in the interfascicular matrix or the fascicular tenocytes. These markers were present in control samples, but staining became increasingly intense with loading. Little MMP‐3 or MMP‐13 was evident in control sections. In loaded samples, some sections showed intense staining of these markers, again localized to interfascicular regions. This study suggests that inflammatory markers may be expressed rapidly after tendon overload exercise. Interestingly, both inflammation and damage‐induced matrix remodeling seem to be concentrated in, or in the vicinity of, the highly cellular interfascicular matrix. © 2015 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. J Orthop Res 33:889–897, 2015.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Both the inflammatory markers selected, IL‐624, 25 and COX‐226, 27 have previously been shown to be upregulated in tendinopathy. MMPs 1 and 13 were selected as they are known to be active against fibrillar collagens, including the predominant collagen type I 1, 28, 29, 30.…”

Section: Methodsmentioning

confidence: 99%

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Spiesz

Thorpe

Chaudhry

et al. 2015

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Although levels of prostaglandin E 2 were not significantly higher in the fluids surrounding painful tendons, 41,42 levels of prostaglandin E 2 and several other inflammatory mediators, such as thromboxane, bradykinin and interleukin (IL)-6, are increased in peritendinous tissue after prolonged exercise. [43][44][45] Other studies have reported increased levels of IL-1 in the tissues surrounding painful tendons, such as the bursa in the shoulder. 46 Increased expression Platelet-rich plasma injection Contains growth factors (e.g.…”

Section: Molecular Pathology Of Tendinopathymentioning

confidence: 98%

Tendinopathy—from basic science to treatment

2008

View full text Add to dashboard Cite

“…Tendon inflammation is marked by the presence of inflammatory mediators, including prostaglandin E 2 (PGE 2 ) and leukotriene B 4 (LTB 4 ) (Almekinders et al, 1993). Although the exact amounts of both PGE 2 and LTB 4 in inflamed tendons are not known, their levels are markedly increased when tendons or tendon fibroblasts are subjected to repetitive mechanical loading conditions (Langberg et al, 1999;Wang et al, 2003;Li et al, 2004). These data suggest that PGE 2 and LTB 4 may play a role in tendon degeneration, the late stages of tendinopathy (Wang, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…PGE 2 is a potent inhibitor of type I collagen synthesis in several connective tissue cell types such as fibroblasts (Varga et al, 1987;Riquet et al, 2000). In addition, elevated PGE 2 levels were found in the human tendon after repetitive mechanical loading (Langberg et al, 1999). Recently, we have shown that exogenous addition of PGE 2 at a dose of 100 ng/ml decreases proliferation and collagen production in human patellar tendon fibroblasts (HPTFs) (Cilli et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Leukotriene B4 at low dosage negates the catabolic effect of prostaglandin E2 in human patellar tendon fibroblasts

Thampatty¹,

Im²,

Wang³

2006

Gene

View full text Add to dashboard Cite

Tendinopathy often involves inflammation and matrix degeneration. The inflammatory mediators such as prostaglandin E 2 (PGE 2 ) and leukotriene B 4 (LTB 4 ) are implicated in the development of tendinopathy. Therefore, the purpose of this study was to determine the effect of PGE 2 and LTB 4 on the proliferation of human patellar tendon fibroblasts (HPTFs), the gene expression of collagen type I, MMP-1 and MMP-3, as well as the protein secretion of these gene products by the cells. The results showed that LTB 4 at low doses (0.1 and 1 nM) significantly increased cell proliferation compared to controls and LTB 4 at 0.1 nM negated the PGE 2 -induced decrease in cell proliferation. In addition, PGE 2 at 100 ng/ml significantly increased the expression of MMP-1 and MMP-3 at both mRNA and protein levels. These stimulatory effects were significantly diminished by co-treatment with LTB 4 at 0.1 nM. Finally, neither PGE 2 nor LTB 4 treatment affected collagen type I gene expression. These results suggest that low levels of LTB 4 counterbalance the negative effects mediated by PGE 2 on tendon fibroblast proliferation and MMP production, which may lead to matrix degradation. Thus, our findings suggest that although LTB 4 is generally thought to be pathogenic, low levels of LTB 4 are actually beneficial in maintaining tendon tissue homeostasis.

show abstract

Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans

Cited by 156 publications

References 42 publications

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Tendinopathy—from basic science to treatment

Leukotriene B4 at low dosage negates the catabolic effect of prostaglandin E2 in human patellar tendon fibroblasts

Contact Info

Product

Resources

About