Metabolism and disposition of 2,2′,4,4′- tetrabromodiphenyl ether following administration of single or multiple doses to rats and mice

Sanders, J. Michael; Chen, L.-J.; Lebetkin, E. H.; Burka, Leo T.

doi:10.1080/00498250500485107

Cited by 50 publications

(81 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No PBDE metabolites were detected in this tissue. Results presented in Table II for BDE153 and for BDE47 and BDE99 presented elsewhere (Sanders et al 2006;Chen et al 2006b), demonstrated an increase in concentrations of all three PBDEs in adipose tissue and liver over time following repeated dosing with the individual congeners. However, relative to each other, concentrations of BDE47 increased, BDE99 decreased, and BDE153 remained constant in adipose tissue over time when administered together (Table III).…”

Section: Resultsmentioning

confidence: 99%

“…Bile was collected from BDE153-treated rats using the method described by Sanders et al (2006). Briefly, each rat (n=2) was anesthetized, the bile duct was isolated and ligated, and bile was collected for 4 h following iv administration of 1 µmol BDE153/kg/ml into a tail vein.…”

Section: Procedures For Collecting Bilementioning

confidence: 99%

“…Other male rats received the prepared radiolabeled mixture of equimolar doses (1 µmol/kg) of BDE47, BDE99, and BDE153 for 1 or three days by gavage. Comparisons were made to animals receiving similar doses of BDE47, BDE99, or BDE153, dosed here or in experiments described in Sanders et al (2006) and Chen et al (2006b). All treated animals were placed into individual metabolism cages for the collection of cumulative urine and feces 24 h following each dose.…”

Section: Disposition Experimentsmentioning

confidence: 99%

“…An equimolar mixture of BDE47, BDE99, and BDE153 (1 µmol/kg of each congener) was prepared in corn oil as previously described for delivery by gavage at 5 ml/kg. Dosing solutions of 1 µmol/kg of radiolabeled BDE47 or BDE99 were made as described in Sanders et al (2006) and Chen et al (2006b). BDE47, BDE99, and BDE153 were administered at ca.…”

Section: Dosing Solutionsmentioning

confidence: 99%

“…Our laboratory has previously investigated the disposition and metabolism of BDE47 and BDE99 in rodents (Sanders et al 2006;Chen et al 2006b). The results of those studies demonstrated quantitative differences in the in vivo fates of a tetra-and pentaBDE; therefore, the present work extends those investigations to a hexaBDE component of the BK70 and DE71 mixtures.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Disposition of 2,2′,4,4′,5,5′-hexabromodiphenyl ether (BDE153) and its interaction with other polybrominated diphenyl ethers (PBDEs) in rodents

et al. 2006

Self Cite

View full text Add to dashboard Cite

1.The disposition of the 14 C-labeled polybrominated diphenyl ether (PBDE), 2,2′,4,4′,5,5′-hexaBDE (BDE153) was investigated in rodents following single and multiple doses and in a mixture with radiolabeled 2,2′,4,4′-tetraBDE (BDE47) and 2,2′,4,4′,5-pentaBDE (BDE99). 2.In single exposure studies, there was little or no effect of dose on BDE153 disposition in male rats in the range of 1-100 µmol/kg. No major sex or species differences in the in vivo fate of BDE153 were detected. BDE153 was: 1) approximately 70% absorbed in rats or mice following gavage. 2) retained in tissues. 3) poorly metabolized and slowly excreted. 3.Mixture studies indicated that, relative to each other, more BDE47 was distributed to adipose tissue, more BDE153 accumulated in liver, and BDE99 was metabolized to the greatest extent. BDE153 was probably retained in liver due to minimal metabolism and elimination after "first pass" distribution to the tissue following gavage.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Procedures For Collecting Bilementioning

confidence: 99%

Section: Disposition Experimentsmentioning

confidence: 99%

Section: Dosing Solutionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Disposition of 2,2′,4,4′,5,5′-hexabromodiphenyl ether (BDE153) and its interaction with other polybrominated diphenyl ethers (PBDEs) in rodents

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

Development of a liquid chromatography/atmospheric pressure photo‐ionization high‐resolution mass spectrometry analytical method for the simultaneous determination of polybrominated diphenyl ethers and their metabolites: application to BDE‐47 metabolism in human hepatocytes

Marteau

Chevolleau

Jouanin

et al. 2012

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Polybrominated diphenyl ethers (PBDEs) are flame retardants widely used in electronic and domestic goods. These persistent pollutants are present in the environment and in humans, and their toxicological properties are of growing concern. PBDEs can be metabolised into compounds suspected to be responsible for their toxicity. These metabolites have been characterised quite well in rodents and fish, but available information in humans remains scarce. For their identification, an efficient method for the simultaneous analysis of PBDEs, hydroxylated PBDEs (OH-PBDEs), and other PBDE metabolites in a single run was needed and has been developed in this work. Atmospheric pressure ionisation modes were compared, and Atmospheric Pressure Photo-Ionization (APPI) was selected. After careful setting of APPI parameters such as dopant and operating temperature, the optimised method was based on APPI ionization coupled to High-Resolution Mass Spectrometry operating in the full scan mode at a resolution of 60 000. This provided excellent sensitivity and specificity, allowing the discrimination of signals which could not be resolved on a triple quadrupole used as a reference. The full-scan high-resolution acquisition mode allowed monitoring of both parent PBDEs and their metabolites, including hydroxylated PBDEs, with detection limits ranging from 0.1 pg to 4.5 pg injected on-column based on the investigated standard compounds. The method was applied to the study of BDE-47 metabolism after incubation with human primary cultures of hepatocytes, and proved to be efficient not only for monitoring the parent compound and expected hydroxylated metabolites, but also for the identification of other non-targeted metabolites. In addition to hydroxy-BDE-47, several conjugated metabolites could be located, and the formation of a dihydrodiol derivative was evidenced for the first time in the case of PBDEs in this work.

show abstract

Brominated diphenyl ether (BDE) levels in liver, adipose, and milk from adult and juvenile rats exposed by gavage to the DE‐71 technical mixture

Bondy

Gaertner

Cherry

et al. 2011

Environmental Toxicology

View full text Add to dashboard Cite

Brominated diphenyl ethers (BDEs) are used as flame retardants in consumer products. Rodent studies indicate that the liver, thyroid, and nervous system of developing animals are targets of BDEs. To explore the relationship between exposure and health in developing animals, BDE accumulation in adult and juvenile rats was examined in conjunction with changes in liver weight and serum thyroxine (T4). Adult (F0) rats received the commercial BDE mixture DE-71 by gavage at doses of 0.5, 5, and 25 mg kg(-1) body weight (bw)/day for 21 weeks. F0 rats were mated and exposure continued throughout breeding, pregnancy, lactation, and postweaning until the pups (F1 generation) reached postnatal day (PND) 42. Milk was collected from lactating dams. Adipose and liver samples were collected from F0 and F1 males and females for BDE congener analysis. Congener prevalence in rat tissues mimicked congener prevalence in wildlife and humans. Tissue concentrations of all congeners except BDE-153 were lower than would be expected based on dose proportionality, confirming that BDE-153 has a high capacity for bioaccumulation. BDEs were transferred from maternal tissues to milk during lactation. Milk congener profiles differed from maternal tissue profiles indicating that degree of bromination and maternal sequestration influenced BDE transfer to milk. Female F1 rats accumulated more BDEs than F1 males, indicating that female rats were less able to metabolize and/or excrete BDEs. Significant effects on liver weight and serum T4 levels were observed in adults and juveniles in the middle and high dose groups, corresponding to BDE levels in the μg g(-1) range. Although it remains to be determined how human liver and thyroid are affected by exposure to much lower BDE levels, the present study confirmed that gender and reproductive status influence BDE accumulation in tissues and BDE transfer to the neonate via milk.

show abstract

Metabolism and disposition of 2,2′,4,4′- tetrabromodiphenyl ether following administration of single or multiple doses to rats and mice

Cited by 50 publications

References 19 publications

Disposition of 2,2′,4,4′,5,5′-hexabromodiphenyl ether (BDE153) and its interaction with other polybrominated diphenyl ethers (PBDEs) in rodents

Disposition of 2,2′,4,4′,5,5′-hexabromodiphenyl ether (BDE153) and its interaction with other polybrominated diphenyl ethers (PBDEs) in rodents

Development of a liquid chromatography/atmospheric pressure photo‐ionization high‐resolution mass spectrometry analytical method for the simultaneous determination of polybrominated diphenyl ethers and their metabolites: application to BDE‐47 metabolism in human hepatocytes

Brominated diphenyl ether (BDE) levels in liver, adipose, and milk from adult and juvenile rats exposed by gavage to the DE‐71 technical mixture

Contact Info

Product

Resources

About