Metabolie Aspects in Mice Administered Live Salmonella typhimurium

Abstract

The influence of intraperitoneal inoculation of live Salmonella typhimurium on carbohydrate and lipid metabolisms in mice was investigated at doses of 9.2 × 107 cells, 1.9 × 108 cells, and 3.8 × 108 cells. The hepatic glycogen content in mice at 18 hr after the inoculation decreased in inverse proportion to an increase in the injection dose. The activities of hepatic phosphorylase and G‐6‐Pase increased significantly after 2 hr, but after 18 hr the levels of both enzyme activities, especially G‐6‐Pase, declined in inverse porportion to an increase in dose of viable cells administered to the mice. The levels of serum glucose and free fatty acids (FFA) in mice markedly decreased at doses of 1.9 × 108 and 3.8 × 108 cells after a transient rise at early stage (1 hr) after the injection. Marked hypertriglyceridemia was seen in infected mice. However, the activity in serum lipoprotein lipase (LPL) was reduced by an increase in the injection dose. The effect of intraperitoneal administration of viable cells on the serum triglyceride level was prevented in mice immunized with S. typhimurium endotoxin or administered with the anti‐endotoxin serum. These results indicate that hypertriglyceridemia mainly results from the action of endotoxin in the pathogen. Serum lactate dehydrogenase (LDH) activity markedly increased at the dose of 3.8 × 108 cells within 8–16 hr, and the infected mice exhibited a leakage of isozymes LDH‐3 and 5 in the serum 16 hr post‐inoculation.

Metabolic Disorders of Serum Lipoproteins in Endotoxin‐Poisoned Mice: The Role of High Density Lipoprotein (HDL) and Triglyceride‐Rich Lipoproteins

“…However, up to now, there have been few reports on serum lipoprotein metabolism in endotoxicosis (2,17,18,21,39,(41)(42)(43)45). The present study was undertaken to determine whether or not there are any differences in the levels of serum lipoprotein or enzymes, especially serum HDL, triglyceride-rich lipoproteins, LCAT and LPL (clearing factor lipase) in mice after endotoxin administration as compared with those in the controls.…”