Metabolically engineered bacteria as light-controlled living therapeutics for anti-angiogenesis tumor therapy

Liu, Xingang; Wu, Min; Wang, Meng; Duan, Yukun; Phan, Chiuyen; Qi, Guobin; Tang, Guping; Liu, Bin

doi:10.1039/d0mh01582b

Cited by 30 publications

(25 citation statements)

References 37 publications

(43 reference statements)

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“…The VEGFR2 protein expression taken by host cells as a foreign antigen activates T-cells’ autoimmune response that targets epithelial cells. This facilitates tumor suppression by acting as an antiangiogenic ( Liu et al, 2021 ).…”

Section: Engineered Bacteria For Disease Managementmentioning

confidence: 99%

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Engineered Bacteria-Based Living Materials for Biotherapeutic Applications

Omer

Mohsin

et al. 2022

Front. Bioeng. Biotechnol.

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Future advances in therapeutics demand the development of dynamic and intelligent living materials. The past static monofunctional materials shall be unable to meet the requirements of future medical development. Also, the demand for precision medicine has increased with the progressively developing human society. Therefore, engineered living materials (ELMs) are vitally important for biotherapeutic applications. These ELMs can be cells, microbes, biofilms, and spores, representing a new platform for treating intractable diseases. Synthetic biology plays a crucial role in the engineering of these living entities. Hence, in this review, the role of synthetic biology in designing and creating genetically engineered novel living materials, particularly bacteria, has been briefly summarized for diagnostic and targeted delivery. The main focus is to provide knowledge about the recent advances in engineered bacterial-based therapies, especially in the treatment of cancer, inflammatory bowel diseases, and infection. Microorganisms, particularly probiotics, have been engineered for synthetic living therapies. Furthermore, these programmable bacteria are designed to sense input signals and respond to disease-changing environments with multipronged therapeutic outputs. These ELMs will open a new path for the synthesis of regenerative medicines as they release therapeutics that provide in situ drug delivery with lower systemic effects. In last, the challenges being faced in this field and the future directions requiring breakthroughs have been discussed. Conclusively, the intent is to present the recent advances in research and biomedical applications of engineered bacteria-based therapies during the last 5 years, as a novel treatment for uncontrollable diseases.

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Section: Engineered Bacteria For Disease Managementmentioning

confidence: 99%

“…(E) Salmonella encodes the VEGFR2 plasmid engineered with MA and promotes anti-angiogenesis therapy of breast cancer in vivo . Reproduced with permission from Liu et al (2021) , Copyright 2021, Royal Society of Chemistry.…”

Section: Engineered Bacteria For Disease Managementmentioning

confidence: 99%

Engineered Bacteria-Based Living Materials for Biotherapeutic Applications

Omer

Mohsin

et al. 2022

Front. Bioeng. Biotechnol.

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“…Besides, AIE PSs have been successfully developed for pathogen detection, discrimination, and treatment. [ 15 ] Therefore, AIE PSs could be promising antibacterial candidates for antibacterial BC fabrication.…”

Section: Introductionmentioning

confidence: 99%

Direct Synthesis of Photosensitizable Bacterial Cellulose as Engineered Living Material for Skin Wound Repair

Liu

Wang

et al. 2022

Advanced Materials

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porosity to support sufficient air exchange. The current clinical approach employed for wound bacterial infection treatment is to apply antibiotics-based wound dressing, which is costly, inefficient, and may lead to antibiotic resistance. [4] Bacterial cellulose (BC), biosynthesized by microorganism fermentation with acetic acid bacteria such as Komagataeibacter sucrofermentans, possesses superior features including high safety, high permeability, high waterholding capacity, excellent flexibility adaptability, low cost, outstanding biocompatibility, and biodegradability. [5] Taking these advantages, BC has been widely applied in artificial skin and blood vessels, scaffolds for tissue engineering, bone regeneration, dental implants, and wound care products. [6] Among these applications, BC is especially suitable for wound dressing because of its 3D coherent nanofiber network structure, leading to ultrahigh porosity and water-holding ability. [6c,7] BC in its natural state does not display any intrinsic antimicrobial capacity, but it can be functionalized with antibacterial agents to realize the infection prevention feature during the wound healing process. [8] Different antibacterial agents such as silver or gold nanoparticles, antibiotics, and chitosan have been attached to BC through physical coating or chemical modification. [9] The physical coating method only requires a moderate modification condition but suffers from shedding of antibacterial moieties from time to time. [10] BC is a natural biopolymer chain composed of β-d-glucose units formed from β-1,4glycosidic bonds. [11] Due to its strong hydrogen bonding and polarizability, BC has poor solubility in conventional organic solvents or water, which often leads to low reaction efficiency for chemical modification. [12] Biosynthesis design for BC by microorganism fermentation has been a new alternative technique that may potentially overcome the limitations of physical coating and chemical modification. [13] Ideally, antibacterial agents could be firmly incorporated into BC during the fermentation process. However, incorporation of an antibacterial agent on BC via microbial biosynthesis is challenging because conventional antibacterial agents could kill the fermentation microorganism. Photosensitizers (PSs), as a class of promising candidates for antibacteria, have no toxicity under dark, but can selectively generate reactive oxygen species (ROS) to induce pathogen lethal injury under light irradiation. [14] Of particular interest are the PSs with aggregationinduced emission characteristics (AIE PSs), which have unique Living materials based on bacterial cellulose (BC) represent a natural and promising candidate for wound dressing. Both physical adsorption and chemical methods have been applied to BC for realizing antibacterial function. However, effective and long-lasting incorporation of bactericidal moieties to BC remains challenging. Herein, a Komagataeibacter sucrofermentans-based direct synthetic method to fabricate photosensitizer-grafted BC thr...

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“…In addition to mammalian cells, large efforts have also been reported for the engineering of bacterial cells. Many different functional materials, including fluorophores, 18,19 photosensitizers, 20,21 polymers, 22,23 biomolecules (unnatural sugars, 24 unnatural amino acids, 18,25 peptides, 26 proteins, 27 nucleic acids, 28 etc. ), nanoparticles, 29,30 as well as extruded cell membranes, 31,32 have been incorporated onto the bacterial surface.…”

Section: Introductionmentioning

confidence: 99%

“…), nanoparticles, 29,30 as well as extruded cell membranes, 31,32 have been incorporated onto the bacterial surface. These artificial functions have enabled a better understanding of natural interactions and realized a wide range of applications including pathogen detection, 33 cell-based therapeutics, 20 and multicellular assembly. 26,27 In addition to the above-mentioned functional materials, DNA as a highly programmable biopolymer has been intensively studied as biological glue to control interactions in 3D space, due to its biorecognition properties, addressability, and ease of functionalization.…”

Section: Introductionmentioning

confidence: 99%