Metabolic utilization of intravenously injected iron from different iron sources in target tissues of broiler chickens

Lü, Lin; Dong, Xue-yu; Ma, Xuelian; Zhang, Jintao; Li, Sufen; Luo, Xugang; Liao, Xiudong

doi:10.1016/j.aninu.2021.06.018

Cited by 8 publications

(9 citation statements)

References 38 publications

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“…Furthermore, the hepatocytes incubated with FeSO4•7H2O, Fe-Met W, and Fe-Prot M had higher Fe concentration than those incubated with Fe-Prot ES group ( Table 1 ). These results are in agreement with the previous study ( Lu et al., 2022 ), which showed that the injected organic Fe source with strong chelation strength was the least favorable for tissue Fe utilization by broilers.…”

Section: Resultssupporting

confidence: 93%

“…Our previous study showed that SDH, COX, and CAT activities in liver increased as dietary supplemental Fe level increased and hepatic SDH, COX and CAT activities were new and sensitive criteria to evaluate the dietary Fe requirements of broilers ( Ma et al, 2016 ). Our current study showed that the Fe sources, added Fe levels and their interaction did not affect SDH, CAT, and COX activities in hepatocytes ( Table 1 ), which is in line with the study of our previous and recent in vivo studies with broilers ( Zhang et al, 2017 ; Lu et al., 2022 ). These results indicated that these enzyme activities lack enough sensitivity to detect differences in metabolic utilization of Fe from different Fe sources in primary cultured hepatocytes of broiler embryos.…”

Section: Resultssupporting

confidence: 93%

“…Additionally, in the present study, the SDH mRNA level in hepatocytes was lower in Fe-Prot ES group than in Fe-Prot M group ( Table 2 ), indicating that Fe-Prot ES might have lower Fe utilization than Fe-Prot M in hepatocytes of broiler embryos. Our recent study also indicated that injected Fe from Fe-Pro ES was less utilizable than that from Fe sulfate, Fe-Met W or Fe-Pro M for hepatic COX mRNA expression of broilers ( Lu et al., 2022 ). These results suggest that although the organic Fe source with greater chelation strength showed higher Fe absorption in small intestine of broilers, their Fe utilization in the liver was not as good as that of the Fe-Prot M and Fe-Met W. This might be due to its extremely strong chelation strength of the bonds between Fe and ligands, which retarded Fe from the organic Fe source being mobilized for metabolic utilization in the liver of broilers.…”

Section: Resultsmentioning

confidence: 96%

“…The liver is the center for the regulation of Fe homeostasis in the body, and hepatic Fe concentration was considered to be a sensitive criterion for assessment of Fe bioavailability ( Lu et al., 2022 ). Furugouri reported that hepatic concentrations of total Fe, non-hemin Fe, hemosiderin Fe and ferritin Fe increased linearly with increasing levels of Fe in pigs ( Furugouri, 1972 ).…”

Section: Resultsmentioning

confidence: 99%

“…Ferritin heavy chain 1 ( FTH1 ) is a 21-kDa subunit of the ferritin complex and plays a vital role in proper Fe 2+ management required for cell growth and function. Our recent study has indicated that hepatic COX mRNA level and splenic FTH1 protein level were sensitive enough for detecting differences in tissue utilization of injected Fe from different Fe sources ( Lu et al., 2022 ). Therefore, we hypothesized that the expression of iron-containing enzyme or protein might be sensitive criteria to detect the differences of metabolic utilization for different Fe sources in primary cultured hepatocytes of broiler embryos.…”

Section: Introductionmentioning

confidence: 99%

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Research Note: Metabolic utilization of iron from different iron sources in primary cultured hepatocytes of broiler embryos

Lü

et al. 2022

Poultry Science

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Research Note: Metabolic utilization of iron from different iron sources in primary cultured hepatocytes of broiler embryos

Lü

et al. 2022

Poultry Science

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Dietary trace mineral pattern influences gut microbiota and intestinal health of broilers

Wang

Chen

et al. 2023

Journal of Animal Science

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Dietary trace minerals can impact gut flora, which can further affect intestinal health. However, dietary balance pattern of trace minerals for intestinal health of broilers needs to be explored. The present study was conducted to investigate the effect of dietary pattern of Cu, Fe, Mn, Zn and Se on the intestinal morphology, microbiota, short chain fatty acid concentrations, antioxidant status and the expression of tight junction proteins in broilers. A total of 240 1-d-old Arbor Acres male broilers were randomly assigned to 1 of 5 treatments with 6 replicate cages of 8 birds per cage for each treatment. The birds were fed the corn-soybean meal basal diet supplemented with 5 combination patterns of trace minerals for 42 d. The dietary treatments were as follows: the inorganic sources were added to the diet based on the recommendations of the current National Research Council (NRC, T1) and Ministry of Agriculture of P. R. China (MAP) (T2) for broiler chicks, respectively; the inorganic sources were added to the diet at the levels based on our previous results of inorganic trace mineral requirements for broilers (T3); the organic sources were added to the diet at the levels considering the bioavailabilities of organic trace minerals for broilers described in our previous studies (T4); and the organic sources were added to the diet based on the recommendations of the current MAP for broiler chicks (T5). The results showed that broilers from T1 had lower (P < 0.05) crypt depth (CD) and higher (P < 0.05) villus height : CD in duodenum on d 21 and lower CD (P < 0.05) in jejunum on d 42 than those from T3 and T4. Broilers from T1, T3 and T5 had higher (P < 0.05) Shannon index in cecum on d 21 than those from T4. Broilers from T1 had higher (P < 0.05) abundance of Lactobacillus in ileum on d 21 than those from T2 and T3. Broilers from T1, T2 and T5 had higher (P < 0.05) valeric acid concentrations in cecum on d 42 than those from T3 and T4. In addition, Birds from T2 had higher (P < 0.05) Claudin-1 mRNA level in jejunum on d 42 than those from T3 and T4. And birds from T3, T4 and T5 had higher (P < 0.05) Occludin protein expression levels in duodenum on d 42 than those from T2. These results indicate that dietary pattern of Cu, Fe, Mn, Zn and Se influenced gut flora and intestinal health of broilers, and the appropriate pattern of Cu, Fe, Mn, Zn and Se in the diet for intestinal health of broilers would be Cu 12 mg, Fe 229 mg, Mn 81 mg, Zn 78 mg and Se 0.24 mg/kg (1 to 21 d of age), and Cu 11 mg, Fe 193 mg, Mn 80 mg, Zn 73 mg and Se 0.22 mg/kg (22 to 42 d of age), when the trace minerals as inorganic sources were added to diets according to the recommendations of the current NRC.

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Iron requirements of broiler chickens as affected by supplemental phytase

Feijo,

Vieira,

Horn

et al. 2023

Journal of Animal Science

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Iron is routinely supplemented in broiler diets intending to prevent dietary deficiencies. The present research was conducted with the objective of assessing Fe requirements of broilers when fed supplemental phytase. A total of 1,280 one-day-old male Cobb x Cobb 500 were distributed in a 2 X 5 factorial arrangement (phytase supplemented diets x 5 graded increases of supplemental Fe) in 80 battery cages, 8 replications of 8 chicks each. The trial was replicated once. Chicks were fed a Fe-deficient diet without phytase (Fe analyzed at 31.30 ± 3.79 mg/kg) from placement to 7 d and then randomly distributed into battery cages with corresponding dieting treatments with or without phytase and graded increases of supplemental Fe. Diets were formulated with corn and soybean meal (SBM), laboratory grade calcium carbonate and phosphoric acid; therefore, the vast majority of dietary Fe originated from corn and SBM (analyzed diet had 53.3 ± 1.41 mg/kg Fe). Phytase was added in excess to the producer recommendation of 1,000 FYT (4,452 ± 487 FYT/kg analyzed) such that phytate degradation was expected to be maximized. Supplemental Fe was from laboratory grade ferrous sulfate heptahydrate (FeSO47H20) which was increasingly added to the diets (analyzed Fe in the supplemented diets were: 53.3 ± 1.41, 65.5 ± 0.59, 77.2 ± 1.97, 87.6 ± 1.72, 97.7 ± 1.33 mg/kg). There were no interactions between phytase and dietary Fe for any response throughout the study (P > 0.05). Supplementing phytase had not effects on Fe intake or Fe excretion, as well as on hematocrit (Ht), hemoglobin (Hb), ferritin, Fe contents in the liver or thigh muscle color (P > 0.05). However, phytase supplemented diets produced better live performance as well as higher ileal digestible energy and Fe digestibility (P<0.05). No effects were found for dietary Fe in live performance at d 28 (P > 0.05). On the other hand, increasing dietary Fe led to linear increases in Fe retention and excretion, Fe contents in livers, as well as Ht and Hb at 14 d (P < 0.05). Quadratic responses (P < 0.05) were observed for Hb at 21d, serum ferritin on d 14, 21 and 28 (maximum responses were 83.3, 104.0, 91.9 and 88.3 mg/kg Fe, respectively). In conclusion, supplementing Fe adding to a total of 97.7 mg/kg dietary Fe did not affect live performance traits. However, the average of Fe related blood parameters was maximized at 91.9 mg/kg dietary Fe. Supplementing phytase provided a significant increase in Fe digestibility.

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Metabolic utilization of intravenously injected iron from different iron sources in target tissues of broiler chickens

Cited by 8 publications

References 38 publications

Research Note: Metabolic utilization of iron from different iron sources in primary cultured hepatocytes of broiler embryos

Research Note: Metabolic utilization of iron from different iron sources in primary cultured hepatocytes of broiler embryos

Dietary trace mineral pattern influences gut microbiota and intestinal health of broilers

Iron requirements of broiler chickens as affected by supplemental phytase

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