Metabolic Tumor Profiling with pH, Oxygen, and Glucose Chemosensors on a Quantum Dot Scaffold

Lemon, Christopher M.; Curtin, Peter N.; Somers, Rebecca C.; Greytak, Andrew B.; Lanning, Ryan M.; Jain, Rakesh K.; Bawendi, Moungi G.; Nocera, Daniel G.

doi:10.1021/ic401587r

Cited by 59 publications

(47 citation statements)

References 279 publications

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“…350,596,597 The QD donor was used as an antennae for the oxygen sensitive emitters as well as being an oxygen insensitive internal standard within a ratiometric signal. Given their vast multiphoton action cross sections, the QDs also served as a two-photon antennae allowing for excitation at longer wavelengths.…”

Section: Aptamermentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials currently utilized within biological applications. However, what is not widely appreciated is their growing role as versatile energy transfer (ET) donors and acceptors within a similar biological context. The progress made on integrating QDs and ET in biological configurations and applications is reviewed in detail here. The goal is to provide the reader with (1) an appreciation for what QDs are capable of in this context, (2) how this field has grown over a relatively short time span, and, in particular, (3) how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates. An initial discussion of QD materials along with key concepts surrounding their preparation and bioconjugation is provided given the defining role these aspects play in the QDs ability to succeed in subsequent ET applications. The discussion is then divided around the specific roles that QDs provide as either Förster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor. For each QD-ET mechanism, a working explanation of the appropriate background theory and formalism is articulated before examining their biosensing and related ET utility. Other configurations such as incorporation of QDs into multistep ET processes or use of initial chemical and bioluminescent excitation are treated similarly. ET processes that are still not fully understood such as QD interactions with gold and other metal nanoparticles along with carbon allotropes are also covered. Given their maturity, some specific applications ranging from in vitro sensing assays to cellular imaging are separated and discussed in more detail. Finally a perspective on how this field will continue to evolve is provided.

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Section: Aptamermentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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“…Experimental results (18,19,22,45,68,72,74,100,112,113,123,125,(129)(130)(131)(134)(135)(136)(137)(138) and theoretical considerations (92,139) suggest nanocrystalline materials and organic-inorganic hybrids are best described in the weak coupling regime. The application of the weak coupling model arises due to the dissimilarity in the wave functions of the molecular orbitals in organic systems and band-like orbitals in nanocrystals that prevents traditional strong interactions.…”

Section: Quantitative Analysis Of Qd Photoluminescence Quenching In Qmentioning

confidence: 97%

“…Typically, the above PL quenching for QD counterpart being observed in numerous QD-dye nanocomposites is commonly interpreted as being due to the photoinduced charge transfer (CT) (23,(119)(120)(121)(122)(123)(124) and/or to the dipole-dipole resonant energy transfer of Foerster type (FRET) QD→dye (18,19,22,45,68,72,74,92,100,101,112,(125)(126)(127)(128)(129)(130)(131). Usually in the FRET case, the direct verification of the energy transfer process as a real reason of PL quenching is the comparison of the experimental values of FRET efficiencies via the donor (QD) PL quenching and the sensitization of the acceptor (porphyrin) fluorescence.…”

Section: Spectral-kinetic Manifestation Of Qd-porphyrin Nanoassembliementioning

confidence: 99%

“…Based on evident progress in the synthesis of colloidal semiconductor QDs with relatively narrow size distributions within a few percent, rational shape-engineering, compositional modulation, electronic doping, and tailored surface chemistries, these nanoobjects as well as nanoassemblies based on QDs and functionalized organic compounds (including proteins) have become an important class of naomaterials with great potential for applications ranging from electronic and optoelectronic devices (1)(2)(3)(4)(5)(6)(7)(8)(9)(10) to material science (11)(12)(13)(14)(15), sensorics (16)(17)(18)(19) and nanomedicine (14,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). The science and technology of nearly all QD-based materials, drug delivery systems, diagnostic methods, controlled release systems, organic-inorganic nanoassemblies, etc., involve on every length scale, from the molecular to the macro, surface and interfacial phenomena that can be tuned by varying the surface and interfacial energy and by varying the specific chemical interactions and chemical groups populating such surfaces and interfaces.…”

Section: Introduction Criteria For Photodynamic Therapy With Qd-basedmentioning

confidence: 99%

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Surface Photochemistry of Quantum Dot-Porphyrin Nanoassemblies for Singlet Oxygen Generation

Zenkevich

Borczyskowski

2015

ACS Symposium Series

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This Chapter covers the basics of nanotechnology, focusing on hybrid organic-inorganic nanoassemblies based on self-assembly principles. Here, we are willing to discuss the formation principles and mechanisms of excitation energy relaxation being studied for nanoassemblies based on core/shell CdSe(ZnS) colloidal semiconductor quantum dots (QD) exhibiting size-dependent photophysical properties and surfacely activated by pyridyl-substituted porphyrins, H 2 P (bulk solutions) in liquid solvents at 295 K (steady-state, picosecond time-resolved luminescence spectroscopy and single molecule spectroscopy). Upon formation of QD-H 2 P nanoassemblies the QD photoluminescence (PL) is quenched as can be detected both via single particle detection and ensemble experiments in solution. PL quenching has been quantitatively assigned to FRET QD→ H 2 P (10-14%) and Non-FRET (86-90%) processes. Using near-IR photoluminescence measurements, we performed a quantitative comparative studying the efficiencies of the singlet oxygen generation by alone QDs and "QD-H 2 P" nanoassemblies upon variation of the laser pulse energy. It was found that for QD-H 2 P nanoassemblies the experimental efficiencies of singlet oxygen generation are essentially higher with respect to those obtained for alone QDs. In the case of QD-H 2 P nanoassemblies, it was proven also that the non-linear dependence of the efficiency of singlet oxygen generation on the laser pulse energy is caused by non-radiative intraband Auger processes, realized in QD counterpart. It has been found that values of FRET QD→ H 2 P efficiencies obtained via direct measurements of IR-emission of singlet oxygen at low laser excitation (Φ IR = 0.12 ± 0.03) are in a good agreement with the corresponding FRET efficiencies found from the direct sensitization data for porphyrin fluorescence (Φ FRET = 0.14 ± 0.02) in nanoassemblies. Such quantitative analysis was done for the first time and shows that namely FRET process QD→H 2 P is a main reason of singlet oxygen generation by QD→H 2 P nanoassemblies.

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“…Ferumoxytol, for example, is an FDA-approved nanomaterial for iron replacement in treating anemia but has been used to enhance MRI (26); when tagged with fluorochromes, ferumoxytol also doubles as an MR and/or optical imaging agent (12). Quantum dots have been essential in certain microscopic imaging experiments (27,28), especially in conjunction with environmentally sensitive particles (29), targeted particles, and short wave infrared particles that can be detected much deeper in tissue (30). Labeled antibodies and antibody fragments have long been used for targeted imaging, and the introduction of long-lived imaging isotopes ( 89 Zr, 68 Ga, 64 Cu, 124 I) has resulted in some spectacular clinical results (31)(32)(33).…”

Section: Engineering Advances Have Yielded Impressive Toolsmentioning

confidence: 99%

Advancing biomedical imaging

Weissleder

Nahrendorf

2015

Proc. Natl. Acad. Sci. U.S.A.

150

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Imaging reveals complex structures and dynamic interactive processes, located deep inside the body, that are otherwise difficult to decipher. Numerous imaging modalities harness every last inch of the energy spectrum. Clinical modalities include magnetic resonance imaging (MRI), X-ray computed tomography (CT), ultrasound, and light-based methods [endoscopy and optical coherence tomography (OCT)]. Research modalities include various light microscopy techniques (confocal, multiphoton, total internal reflection, superresolution fluorescence microscopy), electron microscopy, mass spectrometry imaging, fluorescence tomography, bioluminescence, variations of OCT, and optoacoustic imaging, among a few others. Although clinical imaging and research microscopy are often isolated from one another, we argue that their combination and integration is not only informative but also essential to discovering new biology and interpreting clinical datasets in which signals invariably originate from hundreds to thousands of cells per voxel.

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Metabolic Tumor Profiling with pH, Oxygen, and Glucose Chemosensors on a Quantum Dot Scaffold

Cited by 59 publications

References 279 publications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Surface Photochemistry of Quantum Dot-Porphyrin Nanoassemblies for Singlet Oxygen Generation

Advancing biomedical imaging

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