Metabolic time-course response after resistance exercise: A metabolomics approach

Abstract

This study analysed the time course of the global metabolic acute response after resistance exercise (RE), with the use of proton nuclear magnetic resonance (H NMR) spectroscopy. Ten young healthy males performed 4 sets of 10 repetitions at 70% of one-repetition maximum in the leg press and knee extension exercises and had the serum metabolome assessed at 5, 15, 30 and 60 min post-RE. Measurements were also performed 1 h earlier and immediately before the exercises, as an attempt to characterise each participant's serum metabolome at rest. One-way ANOVA was applied and the significance level was set at P ≤ 0.05. RE promoted an increase in 2-hydroxybutyrate, 2-oxoisocaproate, 3-hydroxyisobutyrate, alanine, hypoxanthine, lactate, pyruvate and succinate concentrations. However, isoleucine, leucine, lysine, ornithine and valine had their concentrations decreased post-RE compared with at rest. This is the first study to show significant changes in serum concentration of metabolites such as 2-oxoisocaproate, 2-hydroxybutyrate, 3-hydroxyisobutyrate, lysine, hypoxanthine and pyruvate post-RE, attesting metabolomics as an interesting approach to advance in the understanding of global RE-induced metabolic changes. Moreover, the present data could influence the time point of blood collection in the future studies that aims to investigate metabolism and exercise.

Exercise‐induced α‐ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1‐dependent adrenal activation

“…We successfully identified several rapid response metabolites (pyruvate, lactate, malate, succinate, AKG, xanthine, and hypoxanthine) induced by resistance exercise. In line with other literature (Yde et al , ; Berton et al , ), we found resistance exercise induced a rapid accumulation of pyruvate and lactate, reflecting anaerobic metabolism and muscle damage (Gorostiaga et al , ). The increase in pyruvate is due to the limited ability of mitochondria to oxidase pyruvate during anaerobic exercise.…”

α-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation

“…We successfully identified several rapid response metabolites (pyruvate, lactate, malate, succinate, AKG, xanthine, and hypoxanthine) induced by resistance exercise. In line with other literature (Berton et al, 2017; Yde et al, 2013), we found resistance exercise induced a rapid accumulation of pyruvate and lactate, reflecting anaerobic metabolism and muscle damage (Gorostiaga et al, 2014). The increase of pyruvate is due to the limited ability of mitochondria to oxidase pyruvate during anaerobic exercise.…”

“…These plasmas metabolic profiles provide signatures of endurance exercise performance and cardiovascular disease susceptibility, and also identify molecular pathways that may modulate the salutary effects on cardiovascular function. However, very few metabolomics data is available for resistance exercise (Berton et al, 2017; Li et al, 2012), and the underlying chemical and physiological mechanisms for the stimulatory effects of resistance exercise on fat loss and muscle hypertrophy are not fully understood. Our goal is to identify the essential mediator for the beneficial metabolic effects of resistance exercise and provide potential therapeutic strategies to mimic the health effects of resistance exercise to combat obesity.…”

Non-targeted metabolomics in sport and exercise science

“…The comprehensive overview of metabolites provided by metabolomic analysis allows for greater certainty that the manipulation of recovery periods did not induce metabolic differences compared with the measurement of a limited number of variables using a traditional approach. More recently, increases in serum lactate, pyruvate, succinate and multiple butyrates, along with a reduction in amino acids, has been recorded after a single bout of resistance exercise (Berton et al, 2016). Urinary increases in lactate, pyruvate and succinate have also been identified as pre-to post-exercise discriminators 30 min after a single 30 s cycle ergometer sprint (Enea et al, 2010).…”