Metabolic studies with 3-methyl glucose. 1. Its fate in the animal body

Campbell, P. N.; Young, F. G.

doi:10.1042/bj0520439

Cited by 32 publications

(9 citation statements)

References 8 publications

(3 reference statements)

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“…The chemical shift is 30% larger than for the signal of D-Glucose-6,6-d 2 , which was reported in recent publications in this field [ 10 , 11 ], making it a little bit easier to distinguish the peaks in the spectrum. OMG has little or no effect on the hormone regulation of the blood sugar level, thus allowing much higher concentrations of this sugar in the blood than for D-glucose [ 21 ]. In the future, this might also allow to increase the SNR.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of deuterium magnetic resonance spectroscopy of 3-O-Methylglucose at 7 Tesla

et al. 2021

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Deuterium Magnetic Resonance Spectroscopy (DMRS) is a non-invasive technique that allows the detection of deuterated compounds in vivo. DMRS has a large potential to analyze uptake, perfusion, washout or metabolism, since deuterium is a stable isotope and therefore does not decay during biologic processing of a deuterium labelled substance. Moreover, DMRS allows the distinction between different deuterated substances. In this work, we performed DMRS of deuterated 3-O-Methylglucose (OMG). OMG is a non-metabolizable glucose analog which is transported similar to D-glucose. DMRS of OMG was performed in phantom and in vivo measurements using a preclinical 7 Tesla MRI system. The chemical shift (3.51 ± 0.1 ppm) and relaxation times were determined. OMG was injected intravenously and spectra were acquired over a period of one hour to monitor the time evolution of the deuterium signal in tumor-bearing rats. The increase and washout of OMG could be observed. Three different exponential functions were compared in terms of how well they describe the OMG washout. A mono-exponential model with offset seems to describe the observed time course best with a time constant of 1910 ± 770 s and an offset of 2.5 ± 1.2 mmol/l (mean ± std, N = 3). Chemical shift imaging could be performed with a voxel size of 7.1 mm x 7.1 mm x 7.9 mm. The feasibility of DMRS with deuterium labelled OMG could be demonstrated. These data might serve as basis for future studies that aim to characterize glucose transport using DMRS.

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Section: Discussionmentioning

confidence: 99%

Feasibility of deuterium magnetic resonance spectroscopy of 3-O-Methylglucose at 7 Tesla

et al. 2021

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“…The nonmetabolizable glucose analog 3-O-methyl-d-glucose (hereafter 3OMd-glucose) has been extensively used as a proxy for d-glucose in the measurement of glucose uptake in vitro (e.g., Sigalet et al 1996;Tavakkolizadeh et al 2001;Chang et al 2004;Lavin et al 2007) and in vivo (e.g., Campbell and Young 1952;Uhing and Kimura 1995a;Chang and Karasov 2004;McWhorter and Karasov 2007;Tracy et al 2007). 3OMd-glucose competes for the same apical cotransporter(s) as dglucose, such as SGLT1 and GLUT2 (Solberg and Diamond 1987;Birnir et al 1991;Uhing and Kimura 1995b;Kellett 2001), and has a maximal transport rate (V max ) in mammals that is 65%-100% that of d-glucose, depending on species (Thomson et al 1982;Birnir et al 1991;Fedorak et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Radiolabeledd‐Glucose and the Nonmetabolizable Analog 3‐O‐Methyl‐d‐Glucose as Tools for In Vivo Absorption Studies

McWhorter¹,

Green²,

Karasov³

2010

Physiological and Biochemical Zoology

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3-O-methyl-D-glucose has been extensively used as a proxy for d-glucose uptake. This nonmetabolizable analog has lower affinity for transporters, potentially leading to underestimates of glucose absorption rates as well as overestimates of the nutritional significance of passive uptake. Here we sought to precisely quantify the bias, if any, incurred when using 3-O-methyl-D-glucose by comparing relative absorption rates with D-glucose in vivo in a seasonally frugivorous bird, the American robin. By simultaneously administering these D-glucose probes with L-glucose--the latter absorbed only via nonmediated mechanisms and the former absorbed by both mediated and nonmediated mechanisms--using common pharmacokinetic procedures, we were able to estimate the nutritional significance of paracellular uptake in this species. The relative absorption rate of 3-O-methyl-D-glucose calculated over the initial absorptive phase was not significantly different from that of D-glucose, indicating that the former provides reasonable estimates of glucose absorption rates in vivo. The ratio of L-glucose to D-glucose cumulative fractional absorption indicates that around 60% of total glucose absorption in American robins is paracellular and showed no apparent bias in using 3-O-methyl-D-glucose when averaged over the entire initial absorptive phase. Although the absorption and elimination kinetics of radiolabeled D-glucose were appropriate for pharmacokinetic analysis in this study, because of the potential for interspecific differences in loss to catabolism, it should be used in vivo with caution and with independent verification of absorption efficiency.

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“…In this method the dissolved material is heated with hydroiodic acid when the methoxyl groups are converted to methyl iodide. I soon showed that the sugar was absorbed from the intestine and was unmetabolized before excretion in the urine with no sign of methylated glycogen (1,2). This was a relatively dull, but not unexpected finding, but a more important one was to emerge.…”

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confidence: 98%

How I Became a Biochemist

Campbell

2002

IUBMB Life

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Metabolic studies with 3-methyl glucose. 1. Its fate in the animal body

Cited by 32 publications

References 8 publications

Feasibility of deuterium magnetic resonance spectroscopy of 3-O-Methylglucose at 7 Tesla

Feasibility of deuterium magnetic resonance spectroscopy of 3-O-Methylglucose at 7 Tesla

Assessment of Radiolabeledd‐Glucose and the Nonmetabolizable Analog 3‐O‐Methyl‐d‐Glucose as Tools for In Vivo Absorption Studies

How I Became a Biochemist

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