Metabolic stability of plants regenerated from cryopreserved shoot tips of Dioscorea deltoidea – an endangered medicinal plant

Dixit-Sharma, Sonali; Ahuja-Ghosh, Sangeeta; Mandal, B. B.; Srivastava, Prem S.

doi:10.1016/j.scienta.2005.02.011

Cited by 23 publications

(5 citation statements)

References 3 publications

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“…The shoot tip of H. perforatum cryopreserved showed genetic stability with sustained production of hypericin after recovery of meristems, and at levels similar to the unfrozen control [189, 190]. Vitrification and encapsulation-dehydration techniques of cryopreservation applied to the Dioscorea deltoidea resulted in high frequency regeneration of plantlets with stability in diosgenin content as the control [191]. The use of minimal growth conservation coupled with genomic DNA methylation manipulation sustained paclitaxel production in the cell cultures of T. media that showed decreased yield upon repeated subculture [192].…”

Section: Stress and Defensive Response Initiators In Relations To Thementioning

confidence: 99%

Stress and defense responses in plant secondary metabolites production

2019

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In the growth condition(s) of plants, numerous secondary metabolites (SMs) are produced by them to serve variety of cellular functions essential for physiological processes, and recent increasing evidences have implicated stress and defense response signaling in their production. The type and concentration(s) of secondary molecule(s) produced by a plant are determined by the species, genotype, physiology, developmental stage and environmental factors during growth. This suggests the physiological adaptive responses employed by various plant taxonomic groups in coping with the stress and defensive stimuli. The past recent decades had witnessed renewed interest to study abiotic factors that influence secondary metabolism during in vitro and in vivo growth of plants. Application of molecular biology tools and techniques are facilitating understanding the signaling processes and pathways involved in the SMs production at subcellular, cellular, organ and whole plant systems during in vivo and in vitro growth, with application in metabolic engineering of biosynthetic pathways intermediates.

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Section: Stress and Defensive Response Initiators In Relations To Thementioning

confidence: 99%

Stress and defense responses in plant secondary metabolites production

2019

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“…Genetic stability was also confirmed using morphological parameters, flow cytometry measurements, and RFLP assays, suggesting that the cryopreservation method does not cause somaclonal variability as no significant differences were observed in regenerated material compared to the controls [33,34]. The metabolic stability of Dioscorea deltoidea and Panax ginseng after cryopreservation was also shown [35]. However, according to other authors, some genetic changes may occur in cryopreserved plants [36][37][38].…”

Section: Introductionmentioning

confidence: 66%

Post-Cryogenic Viability of Peach (Persica vulgaris Mill.) Dormant Buds from the VIR Genetic Collection

et al. 2022

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The long-term storage of the genetic resources of fruit crops for breeding needs can be freely developed by cryopreservation cuttings with dormant buds in liquid nitrogen vapor, but so far, this method has not been practically used for peach. Cuttings with dormant buds of five peach varieties growing in the field gene bank at Krymsk Experiment and Breeding Station of VIR were collected for cryopreservation in 2019–2021. The three-factor analysis of variance showed that the viability of peach cuttings was significantly affected by the year (p < 0.001) and variety (p < 0.001). According to the three-year average characteristics of the cultivars, the analysis of variance showed a significant difference in the viability of the cultivars after cryopreservation (p = 0.004). According to the results of the three years of study, cvs. ‘Podarok Kryma’ (43.3%) and ‘Lucky 24 B’ (44.4%) showed the lowest viability after cryopreservation, significantly lower than cvs. ‘Baby Gold’ (54.4%) and ‘Ustojchivy 90′ (55.6%). Cv. ‘Lyubimets Krasnodara’ (48.9%) occupied an intermediate position. These viability values exceeded the minimum requirement for samples subjected to long-term cryogenic storage in a cryobank. Low-temperature storage of peach cuttings at –5 °C can be used for short-term preservation. After low-temperature storage, the viability of peach cutting amounted to an average of 67.1%.

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“…The most widely used methods for cryopreservation of various endemic and threatened plant species were droplet-vitrification [104], vitrification [105,106], encapsulation-vitrification [107], and encapsulation-dehydration [108]. Different types of explants have been used for cryostorage, such as: shoot tips [109], nodal explants [110], in vitro grown buds [111], callus [104], protocorms [112], seeds [113,114], and pollen [115].…”

Section: Cryopreservationmentioning

confidence: 99%

Overview of the Success of In Vitro Culture for Ex Situ Conservation and Sustainable Utilization of Endemic and Subendemic Native Plants of Romania

Radomir¹,

Stan²,

Florea³

et al. 2023

Sustainability

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Romania has a relatively high diversity of plant species, including 3829 vascular and 979 non-vascular spontaneous plant taxa (species and subspecies). Due to uncontrolled harvesting as well as other causes, including climate change and ecological collapse, the speed of species extinction and the narrowing of the genetic base of plant resources has been reported as a critical issue. Therefore, the national Red List of Romanian flora includes 1453 threatened taxa, of which 95 are endemic and 90 subendemic. Many of these have high ornamental, medicinal–cosmetic, and/or aromatic properties. The high extinction risk of these valuable plants has stimulated both the reconsideration of their vital importance as genetic resources and interest in finding effective methods for conservation. Cultivating these phytogenetic resources in a human-controlled environment is of high importance for effective ex situ conservation, which can further serve sustainable exploitation needs and may facilitate in situ conservation actions. In vitro culture is a powerful tool for producing elite plants for cultivation for different purposes. This review summarizes the current knowledge on in vitro multiplication of 22 endemic and subendemic native plants of Romania, examining the materials used, the treatments applied, and the results obtained in each stage of the micropropagation protocol (culture initiation, proliferation, rooting, and acclimatization). The findings from the reviewed studies are presented in a comparative way, and the potential of plant tissue culture in conservation and sustainable exploitation of these Romanian species is outlined.

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Metabolic stability of plants regenerated from cryopreserved shoot tips of Dioscorea deltoidea – an endangered medicinal plant

Cited by 23 publications

References 3 publications

Stress and defense responses in plant secondary metabolites production

Stress and defense responses in plant secondary metabolites production

Post-Cryogenic Viability of Peach (Persica vulgaris Mill.) Dormant Buds from the VIR Genetic Collection

Overview of the Success of In Vitro Culture for Ex Situ Conservation and Sustainable Utilization of Endemic and Subendemic Native Plants of Romania

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