Metabolic risks of neonates at birth following in utero exposure to HIV-ART: the amino acid profile of cord blood

Moutloatse, Gontse P.; Schoeman, Johannes C.; Lindeque, Jeremie Zander; Reenen, Mari van; Hankemeier, Thomas; Bunders, Madeleine J.; Mason, Shayne

doi:10.1007/s11306-017-1222-y

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2023

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(2 citation statements)

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“…Sample preparation and LC-MS/MS analysis was done based on the methods described in detail by Moutloatse et al (2017) [ 18 ], with slight deviation. Briefly, a 50 μL serum sample was mixed with an ice cold 410 μL isotope mixture in methanol and acetonitrile (1:5 ratio), centrifuged (protein crash) and supernatant dried under nitrogen gas.…”

Section: Methodsmentioning

confidence: 99%

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Phenylalanine metabolism and tetrahydrobiopterin bio-availability in COVID-19 and HIV

Mason

Reenen

Rossouw

et al. 2023

Heliyon

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Section: Methodsmentioning

confidence: 99%

“…One μl sample was injected and analyzed with a 1200 series Agilent HPLC system linked to an Agilent 6410 MSMS system. The settings for the LC-MS/MS were identical to those of Moutloatse et al (2017) [ 18 ].…”

Section: Methodsmentioning

confidence: 99%