Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations

Sousa, Andressa Rodrigues de Oliveira; Silva, Edson Mário de Andrade; Filho, M. A. Coelho; Costa, Márcio Gilberto Cardoso; Filho, Walter dos Santos Soares; Micheli, Fabienne; Maserti, Biancaelena; Gesteira, Abelmon da Silva

doi:10.1016/j.scienta.2021.110490

Cited by 18 publications

(10 citation statements)

References 53 publications

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“…Trehalose – an osmoprotective disaccharide with the ability to stabilize dehydrated membranes (Golovina et al, 2009; Luo et al, 2010) – was also accumulated in roots of all rootstocks, reinforcing previous observation (Santana-Vieira et al, 2016). Unlike previous studies reporting higher raffinose in roots and leaves (Santana-Vieira et al, 2016; Sousa et al, 2022), raffinose accumulation was found only in leaves, mainly young leaves under water deficit ( Figs. 5b and 7 ).…”

Section: Discussioncontrasting

confidence: 99%

“…In drought tolerant plants, increased IAA biosynthesis was associated with an up-regulation of indole-3-acetaldehyde oxidase, a key enzyme in the tryptophan degradation pathway (Gong et al, 2010; Pustovoitova et al, 2004). In the other rootstocks, tryptophan was increased, as already reported for Sunki mandarin (Sousa et al, 2022). In addition, decreases in all phenylpropanoids ( Figs.…”

Section: Discussionsupporting

confidence: 80%

“…Citrus rootstocks presented differential responses and strategies under water deficit. As reported in previous studies (Santana-Vieira et al, 2016; Sousa et al, 2022), Sunki mandarin rootstock seemed to invest more energy in protective compounds and had impaired plant growth, while Swingle citrumelo resisted against drought and presented minor metabolic changes. Rangpur lime responded earlier to the initial variation of water availability, prioritizing root growth rather than shoot growth, a physiological strategy to balance water uptake and loss when such resource is limiting.…”

Section: Discussionsupporting

confidence: 72%

“…Although the metabolic profile has already been used to understand drought tolerance in citrus species (Pérez-Pérez et al, 2007; Zandalinas et al, 2017; Sousa et al, 2022), new insights are only possible if a comprehensive spatio-temporal analysis is performed considering water deficit intensity and duration, plant recovery capacity, plant organs, and modulation by rootstocks in citrus trees. Assuming that the morpho-physiological changes already identified in Rangpur lime are associated with the reconfiguration of metabolic pathways, the identification of key metabolites – and then metabolic pathways – associated with drought tolerance and how such reconfiguration affects scion metabolism become essential to reveal the rootstock modulation of citrus responses to drought and then link micro (cellular level) to macro (plant level) changes under varying water conditions.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability

Silva

Miranda

Cunha

et al. 2022

Preprint

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Water stress is a major environmental factor affecting Citrus spp. and Rangpur lime is a drought-tolerant rootstock used to enhance orange yield in rainfed orchards. Here, we combined morpho-physiological analyses with metabolic profiling of roots and leaves of Valencia orange scions grafted onto Rangpur lime, Swingle citrumelo or Sunki mandarin rootstocks under water deficit. Our aim was to present a comprehensive spatio-temporal evaluation of citrus responses to drought and highlight the metabolic adjustments associated with drought tolerance induced by Rangpur lime. Plant responses were evaluated during the initial phase of reduction in water availability, when water deficit was maximum and also after rehydration. Fifty-eight primary metabolites were modulated by water deficit, mainly amino acids, organic acids and sugars. Metabolic changes indicated adjustments related to osmotic, energetic and redox processes under low water availability, which were dependent on rootstock and varied between roots and leaves and along the experimental period. Rangpur lime prioritized root growth in the initial phase of water deficit, which was linked to less sugar accumulation, changes in nucleotide metabolism, downregulation in Shikimic acid pathway and accumulation of arginine. After rehydration, the resume of shoot growth was associated with high accumulation of arginine and asparagine. The better performance of Rangpur lime seems to be associated with its high sensitivity of roots to changes in water availability and possible signaling compounds have been suggested.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

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Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability

Silva

Miranda

Cunha

et al. 2022

Preprint

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“…Studies have shown that most flavonoid biosynthesis-related genes were up-regulated in grafted plants (Lopez-Hinojosa et al, 2021). Furthermore, it has been reported that trehalose and raffinose in the citrus rootstock, and sugar alcohols (e.g., sorbitol) and amino acids (e.g., tyrosine, aspartic acid, methionine, ornithine, and tryptophan) in droughtresistant rootstocks could support the antioxidant system and scavenge ROS to protect the membrane integrity (Jimenez et al, 2013;Keunen et al, 2013;Lunn et al, 2014;Santana-Vieira et al, 2016;Sousa et al, 2022). It also indicated that the proline accumulation in the plants grafted with drought-resistant rootstocks could stabilize the membrane and proteins under water-deficient conditions (Jimenez et al, 2013;Schneider et al, 2018;Ozturk et al, 2021).…”

Section: Non-enzymatic Antioxidantsmentioning

confidence: 99%

Grafting enhances plants drought resistance: Current understanding, mechanisms, and future perspectives

Yang

Xia²,

Zeng³

et al. 2022

Front. Plant Sci.

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Drought, one of the most severe and complex abiotic stresses, is increasingly occurring due to global climate change and adversely affects plant growth and yield. Grafting is a proven and effective tool to enhance plant drought resistance ability by regulating their physiological and molecular processes. In this review, we have summarized the current understanding, mechanisms, and perspectives of the drought stress resistance of grafted plants. Plants resist drought through adaptive changes in their root, stem, and leaf morphology and structure, stomatal closure modulation to reduce transpiration, activating osmoregulation, enhancing antioxidant systems, and regulating phytohormones and gene expression changes. Additionally, the mRNAs, miRNAs and peptides crossing the grafted healing sites also confer drought resistance. However, the interaction between phytohormones, establishment of the scion-rootstock communication through genetic materials to enhance drought resistance is becoming a hot research topic. Therefore, our review provides not only physiological evidences for selecting drought-resistant rootstocks or scions, but also a clear understanding of the potential molecular effects to enhance drought resistance using grafted plants.

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Asparagine and nitric oxide jointly enhance antioxidant capacity and nitrogen metabolism to improve drought resistance in cotton: Evidence from long‐term field trials

Akin,

Kaya

2023

Food and Energy Security

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Asparagine (Asn) and nitric oxide (NO) can enhance plant tolerance to abiotic stress, but their interaction is not well understood. Cotton is a vital resource for the textile industry, but its yield is reduced by drought stress, which could threaten its global supply in a warming and stressful world. Improving cotton's drought tolerance is crucial for supporting the textile industry. Two simultaneous field experiments were conducted to study the effect of Asn and sodium nitroprusside (SNP), a NO donor, on cotton's drought tolerance. Two irrigation treatments were applied: control (C: 100% A pan) and drought stress (50% A pan). The plants were also sprayed with two plant stimulants before imposing drought stress: Mock control, 20 mM Asn, and 0.2 mM SNP, either alone or together. Drought stress impaired plant growth, photosynthesis, yield, nitrogen metabolism, and antioxidant defense, while increasing oxidative stress and free amino acid levels. However, Asn and SNP treatments alleviated these negative effects and improved antioxidant enzyme activity, plant growth, yield, and nitrogen content. The Asn + SNP treatment also increased irrigation water productivity under water‐limited conditions, suggesting its potential for enhancing water use efficiency in cotton production. The combined treatment was more effective than the single treatments, indicating a synergistic effect of Asn and SNP in enhancing drought tolerance in cotton. These results imply that Asn and SNP could be useful tools for sustaining cotton production under drought conditions by boosting nitrogen metabolism and antioxidant defense, thereby contributing to the global supply of cotton and supporting the textile industry.

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Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations

Cited by 18 publications

References 53 publications

Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability

Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability

Grafting enhances plants drought resistance: Current understanding, mechanisms, and future perspectives

Asparagine and nitric oxide jointly enhance antioxidant capacity and nitrogen metabolism to improve drought resistance in cotton: Evidence from long‐term field trials

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