Metabolic Responses of a Phototrophic Co-Culture Enriched from a Freshwater Sediment on Changing Substrate Availability and its Relevance for Biogeochemical Iron Cycling

Schmidt, Caroline; Nikeleit, Verena; Schaedler, Franziska; Leider, Arne; Lueder, Ulf; Bryce, Casey; Hallmann, Christian; Kappler, Andreas

doi:10.1080/01490451.2020.1837303

Cited by 3 publications

(8 citation statements)

References 74 publications

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“…Nevertheless, there is the possibility for a close relationship with the Pseudopelobacter sp., much like what was observed by Schmidt et al (2021) in their enrichment. It seems unlikely to involve simultaneous Fe(III) reduction by Pseudopleobacter sp.…”

Section: Morphology and Growth Of Bla1supporting

confidence: 80%

“…Here, we describe the enrichment and characterization of a novel pelagic Fe(II)-oxidizing photoferrotroph from a meromictic and ferruginous lake that is closely related to other phototrophic GSB, but distinct in genomic and physiological characteristics. Similar to other photoferrotrophic GSB (Heising et al, 1999;Bryce et al, 2019;Schmidt et al, 2021), this organism could not be isolated, but is the most abundant organism in the enrichment. The characterization of novel photoferrotrophs informs the framework for understanding how APB, specifically GSB, influence carbon and iron cycling within ferruginous systems.…”

Section: Introductionmentioning

confidence: 83%

“…This is similar to the optimum for other photoferrotrophs (Straub et al, 1999), although a freshwater photoferrotrophic Chlorobium sp. isolated from Lake Constance had an optimum of pH 7.4-7.6 (Schmidt et al, 2021). Protons are a product of Eq.…”

Section: Morphology and Growth Of Bla1mentioning

confidence: 99%

“…with a photosynthetic Rhodopseudomonas sp. (Schmidt et al, 2021). In that study, detailed experiments and subsequent isolation of the Rhodopseudomonas sp.…”

Section: Morphology and Growth Of Bla1mentioning

confidence: 99%

“…strain N1 enrichment (Laufer et al, 2017;Bryce et al, 2019), the freshwater Chlorobium ferrooxidans strain KoFox, which grows in coculture with Geospirillum sp. strain KoFum (Heising et al, 1999), the freshwater coculture of a strain closely related to Chlorobium ferrooxidans that grows in coculture with a strain closely related to Rhodopseudomonas palustris (Schmidt et al, 2021), and the isolate Chlorobium phaeoferrooxidans strain KB01 (Crowe et al, 2017). C. phaeoferrooxidans strain KB01 is the first photoferrotroph to be isolated from a ferruginous water column (Llirós et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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“Candidatus Chlorobium masyuteum,” a Novel Photoferrotrophic Green Sulfur Bacterium Enriched From a Ferruginous Meromictic Lake

et al. 2021

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Anoxygenic phototrophic bacteria can be important primary producers in some meromictic lakes. Green sulfur bacteria (GSB) have been detected in ferruginous lakes, with some evidence that they are photosynthesizing using Fe(II) as an electron donor (i.e., photoferrotrophy). However, some photoferrotrophic GSB can also utilize reduced sulfur compounds, complicating the interpretation of Fe-dependent photosynthetic primary productivity. An enrichment (BLA1) from meromictic ferruginous Brownie Lake, Minnesota, United States, contains an Fe(II)-oxidizing GSB and a metabolically flexible putative Fe(III)-reducing anaerobe. “Candidatus Chlorobium masyuteum” grows photoautotrophically with Fe(II) and possesses the putative Fe(II) oxidase-encoding cyc2 gene also known from oxygen-dependent Fe(II)-oxidizing bacteria. It lacks genes for oxidation of reduced sulfur compounds. Its genome encodes for hydrogenases and a reverse TCA cycle that may allow it to utilize H2 and acetate as electron donors, an inference supported by the abundance of this organism when the enrichment was supplied by these substrates and light. The anaerobe “Candidatus Pseudopelobacter ferreus” is in low abundance (∼1%) in BLA1 and is a putative Fe(III)-reducing bacterium from the Geobacterales ord. nov. While “Ca. C. masyuteum” is closely related to the photoferrotrophs C. ferroooxidans strain KoFox and C. phaeoferrooxidans strain KB01, it is unique at the genomic level. The main light-harvesting molecule was identified as bacteriochlorophyll c with accessory carotenoids of the chlorobactene series. BLA1 optimally oxidizes Fe(II) at a pH of 6.8, and the rate of Fe(II) oxidation was 0.63 ± 0.069 mmol day–1, comparable to other photoferrotrophic GSB cultures or enrichments. Investigation of BLA1 expands the genetic basis for phototrophic Fe(II) oxidation by GSB and highlights the role these organisms may play in Fe(II) oxidation and carbon cycling in ferruginous lakes.

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Section: Morphology and Growth Of Bla1supporting

confidence: 80%

Section: Introductionmentioning

confidence: 83%

Section: Morphology and Growth Of Bla1mentioning

confidence: 99%

“…with a photosynthetic Rhodopseudomonas sp. (Schmidt et al, 2021). In that study, detailed experiments and subsequent isolation of the Rhodopseudomonas sp.…”

Section: Morphology and Growth Of Bla1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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“Candidatus Chlorobium masyuteum,” a Novel Photoferrotrophic Green Sulfur Bacterium Enriched From a Ferruginous Meromictic Lake

et al. 2021

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Microbial community dynamics and cycling of plutonium and iron in a seasonally stratified and radiologically contaminated pond

Merino,

Wasserman,

Coutelot

et al. 2023

Sci Rep

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Plutonium (Pu) cycling and mobility in the environment can be impacted by the iron cycle and microbial community dynamics. We investigated the spatial and temporal changes of the microbiome in an iron (Fe)-rich, plutonium-contaminated, monomictic reservoir (Pond B, Savannah River Site, South Carolina, USA). The microbial community composition varied with depth during seasonal thermal stratification and was strongly correlated with redox. During stratification, Fe(II) oxidizers (e.g., Ferrovum, Rhodoferax, Chlorobium) were most abundant in the hypoxic/anoxic zones, while Fe(III) reducers (e.g., Geothrix, Geobacter) dominated the deep, anoxic zone. Sulfate reducers and methanogens were present in the anoxic layer, likely contributing to iron and plutonium cycling. Multinomial regression of predicted functions/pathways identified metabolisms highly associated with stratification (within the top 5%), including iron reduction, methanogenesis, C1 compound utilization, fermentation, and aromatic compound degradation. Two sediment cores collected at the Inlet and Outlet of the pond were dominated by putative fermenters and organic matter (OM) degraders. Overall, microbiome analyses revealed the potential for three microbial impacts on the plutonium and iron biogeochemical cycles: (1) plutonium bioaccumulation throughout the water column, (2) Pu–Fe-OM-aggregate formation by Fe(II) oxidizers under microaerophilic/aerobic conditions, and (3) Pu–Fe-OM-aggregate or sediment reductive dissolution and organic matter degradation in the deep, anoxic waters.

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Phototrophic Fe(II) oxidation benefits from light/dark cycles

Nikeleit,

Roth,

Maisch

et al. 2024

Environ Microbiol Rep

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Phototrophic Fe(II)‐oxidizers use Fe(II) as electron donor for CO2 fixation thus linking Fe(II) oxidation, ATP formation, and growth directly to the availability of sunlight. We compared the effect of short (10 h light/14 h dark) and long (2–3 days light/2–3 days dark) light/dark cycles to constant light conditions for the phototrophic Fe(II)‐oxidizer Chlorobium ferrooxidans KoFox. Fe(II) oxidation was completed first in the setup with constant light (9 mM Fe(II) oxidised within 8.9 days) compared to the light/dark cycles but both short and long light/dark cycles showed faster maximum Fe(II) oxidation rates. In the short and long cycle, Fe(II) oxidation rates reached 3.5 ± 1.0 and 2.6 ± 0.3 mM/d, respectively, compared to 2.1 ± 0.3 mM/d in the constant light setup. Maximum Fe(II) oxidation was significantly faster in the short cycle compared to the constant light setup. Cell growth reached roughly equivalent cell numbers across all three light conditions (from 0.2–2.0 × 106 cells/mL to 1.1–1.4 × 108 cells/mL) and took place in both the light and dark phases of incubation. SEM images showed different mineral structures independent of the light setup and 57Fe Mössbauer spectroscopy confirmed the formation of poorly crystalline Fe(III) oxyhydroxides (such as ferrihydrite) in all three setups. Our results suggest that periods of darkness have a significant impact on phototrophic Fe(II)‐oxidizers and significantly influence rates of Fe(II) oxidation.

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Metabolic Responses of a Phototrophic Co-Culture Enriched from a Freshwater Sediment on Changing Substrate Availability and its Relevance for Biogeochemical Iron Cycling

Cited by 3 publications

References 74 publications

“Candidatus Chlorobium masyuteum,” a Novel Photoferrotrophic Green Sulfur Bacterium Enriched From a Ferruginous Meromictic Lake

“Candidatus Chlorobium masyuteum,” a Novel Photoferrotrophic Green Sulfur Bacterium Enriched From a Ferruginous Meromictic Lake

Microbial community dynamics and cycling of plutonium and iron in a seasonally stratified and radiologically contaminated pond

Phototrophic Fe(II) oxidation benefits from light/dark cycles

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