Metabolic response to prolonged cycling with 13C-glucose ingestion following downhill running

Abstract

The purpose of this study was to describe the effect of muscle damage and delayed-onset muscle soreness (DOMS) on the metabolic response during a subsequent period of prolonged concentric exercise (120 min, approximately 61% V(.)O(2max), on a cycle ergometer), with ingestion of 3 g of (13)C-glucose/kg body mass. We hypothesized that the oxidation of plasma and exogenous glucose would be reduced, while the oxidation of glucose arising from muscle glycogen would be increased. Six male subjects were studied during exercise in a control situation and 2 days following downhill running, at a time when plasma creatine kinase (CK) activity was increased, and DOMS was present. Carbohydrate and lipid oxidation were computed from indirect respiratory calorimetry corrected for protein oxidation, while the oxidation of plasma glucose and muscle glycogen were computed from V(.)(13)CO(2) and the ratio of (13)C/(12)C in the plasma glucose. All data were presented as the mean and the standard error of the mean. The oxidation of protein (approximately 6% energy yield, in the control and the experimental trial), lipid (approximately 15 and approximately 18%), and carbohydrate (approximately 79 and approximately 76%), as well as that of plasma glucose (approximately 41 and approximately 46%), glucose from the liver (approximately 12 and approximately 14%), and glucose from muscle glycogen (approximately 38 and approximately 31%) were not significantly different between the control and experimental (DOMS) trials. The response of the plasma glucose, insulin, lactate, and free fatty acid concentrations was not modified by the previous eccentric exercise. These results indicate that the metabolic response to prolonged concentric exercise is not modified by muscle damage and DOMS resulting from a bout of eccentric exercise performed 2 days before.

Muscle Fatigue Increases the Probability of Developing Hyperalgesia in Mice

“…13,33 However, the current study was unable to detect changes in lactate, phosphate, creatinine kinase, or to detect infiltration of neutrophils in the muscle, suggesting that metabolic changes in the muscle associated with damage are unlikely to contribute to the enhanced nociceptive response to pH. Changes in blood lactate clearly occur during a fatiguing exercise bout, 12,16,34,37 and these lactate changes can last up to 15 minutes after a high-intensity exercise bout. 16,17,37 In the current study, the animals spontaneously ran in the running wheel and thus were not likely exercising at a strong intensity typically observed in studies that measure lactate.…”