Metabolic response of tomato leaves upon different plant–pathogen interactions

López-Gresa, María Pilar; Maltese, Federica; Bellés, José M.; Conejero, Vicente; Kim, Hye Kyong; Choi, Young Hae; Verpoorte, Robert

doi:10.1002/pca.1179

Cited by 115 publications

(99 citation statements)

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“…The results previously found in P syringae-infected-tomato leaves, in which in general high levels of some amino acids were found, (Pérez Garcia et al, 1998;López-Gresa et al, 2010), are in contrast with those found in this work upon viral infection which produced a general decrease in the pool of amino acids. Only glutamine/glutamate and asparagine/aspartate were clearly induced in leaves inoculated 345 with ToMV.…”

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confidence: 56%

“…1 H NMR spectra, 2D NMR experiments (2D J-resolved, COSY and HMBC), our in-house database of reference compounds and previous reports (López-Gresa et al, 2010) were used for the signal assignment of metabolites from the tomato leaves. Table 1 (Fig 2): b) leaves located at different positions in the stem by component 2 (Fig 2); c) healthy control and ToMV infected leaves by 280 component 3 (Fig 3); and d) samples collected at 9:00 h and 19:00 h by component 4 (Fig 4).…”

Section: Plant Metabolites Have Very Diverse Physical-chemical Propermentioning

confidence: 99%

“…In our previous report, some characteristic metabolites of tomato plants related to either Citrus Exocortis viroid (CEVd) or Pseudomonas syringae infection have been identified by NMR-based metabolomics (López-Gresa et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

López-Gresa

Lisón

Kim

et al. 2012

Journal of Plant Physiology

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general time-dependent decrease in organic acids, amino acids (excluding asparagine), phenylpropanoids and rutin was observed in individual leaves. In addition, metabolite alterations were also found to correlate with the developmental stage of the leaf: high levels of organic acids, some amino acids, phenylpropanoids, and flavonoids were found in lower leaves while elevated amounts of sugars were present in the upper ones. 35Moreover, a marked variation in the content of some metabolites was also observed to be associated to the asymptomatic ToMV infection both in inoculated and systemically infected leaves. While flavonoids accumulated in virus-inoculated leaves, increased levels of phenylpropanoids were observed in non-inoculated leaves where ToMV actively replicates. Finally, diurnal changes in the metabolite content were also 40 observed: an increase of amino acids and organic acids (except glutamic acid) were observed in the samples collected in the morning, whereas sugars and secondary metabolite levels increased in the tomato leaves harvested in the evening.

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confidence: 56%

Section: Plant Metabolites Have Very Diverse Physical-chemical Propermentioning

confidence: 99%

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Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

López-Gresa

Lisón

Kim

et al. 2012

Journal of Plant Physiology

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“…Moreover, a number of studies have demonstrated that exogenous treatments with SA induce resistance to different pathogens in different species (Edgar et al, 2006;Mandal et al, 2009;Meher et al, 2011;Saikia et al, 2003;Shang et al, 2011;Spletzer and Enyedi, 1999;Wang and Liu, 2012). Studies with different plant-pathogen systems establishing a compatible, non-necrotizing interaction, have reported a significant accumulation of gentisic acid (GA) in infected plants (Bellés et al, 1999;Bellés et al, 2006;López-Gresa et al, 2010). Furthermore, exogenous applications of this compound induce defensive responses different than those mediated by salicylic acid (SA).…”

Section: Discussionmentioning

confidence: 99%

Salicylic acid and gentisic acid induce RNA silencing-related genes and plant resistance to RNA pathogens

Campos

Granell

Tárraga

et al. 2014

Plant Physiology and Biochemistry

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ElsevierCampos Beneyto, L.; Granell Albert, P.; Tarraga Herrero, S.; López Gresa, MP.; Conejero Tomás, V.; Belles Albert, JM.; Rodrigo Bravo, I.... (2014) AbstractWe have observed that treatments with salicylic acid (SA) or gentisic acid (GA) induced resistance to RNA pathogens such as ToMV and CEVd in tomato and Gynura auriantiaca, respectively. Accumulation of SA and GA has been found to occur in plants infected by these pathogens, thus pointing out a possible defence role of both molecules. To study the molecular basis of the observed induced resistance to RNA pathogens the induction of silencing-related genes by SA and GA was considered. For that purpose, we searched for tomato genes which were orthologous to those described in Arabidopsis thaliana, such as AtDCL1, AtDCL2, AtDCL4, AtRDR1, AtRDR2 and AtRDR6, and we tracked their induction in tomato along virus and viroid infections. We observed that CEVd significantly induced all these genes in tomato, with the exception of ToRDR6, being the induction of ToDCL4 the most outstanding. Regarding the ToMV asymptomatic infection, with the exception of ToRDR2, we observed a significant induction of all the indicated silencing-related genes, being ToDCL2 the most induced gene. Subsequently, we analyzed their transcriptional activation by SA and at the time when ToMV was inoculated on plants. ToDCL2, ToRDR1 and ToRDR2 were significantly induced by both SA and GA, whereas ToDCL1 was only induced by SA. Such an induction resulted more effective by SA treatment, which is in agreement with the stronger SA-induced resistance observed. Our results suggest that the observed delay in the RNA pathogen accumulation could be due to the pre-induction of RNA silencing-related genes by SA or GA. Highlights-Treatments with salicylic acid or gentisic acid induce resistance to RNA pathogens -Induction of tomato silencing-related genes by RNA pathogen infections is studied -Induction of RNA silencing by SA or GA treatments is analysed in tomato plants -New connexions between RNA silencing and the SA or GA response are proposed

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“…In previous studies Zacarés et al, 2007;Bellés et al, 2008;López-Gresa et al, 2010), we have determined the chemical structure, accumulation kinetics, and functional significance of diverse compounds from the phenylpropanoid metabolism in tomato and cucumber infected with different pathogens. In particular, challenging Rutgers tomato with P. syringae pv.…”

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confidence: 99%

Identification of defence metabolites in tomato plants infected by the bacterial pathogen Pseudomonas syringae

López-Gresa

Torres

Campos

et al. 2011

Environmental and Experimental Botany

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being associated with the tomato/P. syringae interaction. Inhibition of the P. syringaeinduced ethylene by 2-aminoethoxyvinylglycine, markedly impaired the accumulation of HCAA in inoculated tomato leaves, but it had no effect on CGA or rutin synthesis.On the other hand, the lack of accumulation of SA in NahG transgenic tomato plants, overexpressing a bacterial salicylic hydroxylase, did not prevent the enhancing of 40 HCAA produced by P. syringae infection. Taken together, our results indicate that ethylene, but not SA, is essential for the synthesis of HCAA in response to bacterial infection of tomato leaves. Antibiotic activities of the induced metabolites were also studied. CGA induced a notable expression of the defence-related genes PR1 and P23.Moreover, trans-HCAA of noradrenaline and octopamine showed a potent free radical 45 scavenging competence. In particular, trans N-feruloylnoradrenaline presented a very 3 outstanding antioxidant activity, thus indicating that these compounds may play a role in the defence response of tomato plants against bacterial infection. 50Keywords: Solanum lycopersicum, Pseudomonas syringae, plant metabolites, plantpathogen interactions, hydroxycinnamic acid amides, free radical scavenging activity.

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Metabolic response of tomato leaves upon different plant–pathogen interactions

Abstract: NMR metabolomics is a potent platform to analyse the compounds involved in different plant infections. A broad response to different pathogenic infections was revealed at metabolomic levels in the plant. Also, metabolic specificity against each pathogen was observed.

Cited by 115 publications

References 23 publications

Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

Salicylic acid and gentisic acid induce RNA silencing-related genes and plant resistance to RNA pathogens

Identification of defence metabolites in tomato plants infected by the bacterial pathogen Pseudomonas syringae

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