Physical injury and tissue damage is prevalent throughout the animal kingdom, with the ability to quickly and efficiently regenerate providing a selective advantage. The skeletal muscle possesses a uniquely large regenerative capacity within most vertebrates, and has thus become an important model for investigating cellular processes underpinning tissue regeneration. The innate immune system, specifically those of the myeloid lineage, have garnered increasing attention as crucial mediators orchestrating the successful resolution of muscle regeneration, most notably their role in phagocytosis of cellular debris, secretion of cytokines regulating muscle stem cell dynamics, and temporal control of the pro- and anti-inflammatory phases. However, investment into the innate immune system is energetically costly resulting in many species decreasing investment in the innate immune system under nutrient-limited environments. Whether this reduced investment into the innate immune system results in a decreased capacity to mount a regenerative response following tissue damage remains unclear. To this point, we utilized the emerging evolutionary model, Astyanax mexicanus, to investigate the consequence of shifts in immune system investment on skeletal muscle regeneration.