Metabolic regulation of T lymphocyte motility and migration

Vuononvirta, Juho; Marelli‐Berg, Federica M.; Poobalasingam, Thanushiyan

doi:10.1016/j.mam.2020.100888

Cited by 14 publications

(6 citation statements)

References 110 publications

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“…Here, both rSsAK-1 and rSsAK-2 promoted PBMC migration, and high concentrations of rSsAK-2 exerted a particularly significant effect, inducing PBMC migration into the skin lesions and participating in the course of local infection. PBMC migration is regulated by many chemokines and adhesion factors ( 31 , 32 ), which in turn are regulated by cytokines ( 33 ). Here, the rSsAKs promoted IL-4 secretion while rSsAK-2 inhibited IL-10 secretion.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of two recombinant arginine kinases in Sarcoptes Scabiei on host peripheral blood mononuclear cells

et al. 2022

Front. Immunol.

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BackgroundAs an important zoonotic parasitic disease with global distribution, scabies causes serious public health and economic problems. Arginine kinase (AK) is involved in cell signal transduction, inflammation, and apoptosis. Two AKs were identified in Sarcoptes scabiei, but their functions in the host immune response remain unclear.MethodsrSsAK-1 and rSsAK-2 were expressed, purified, and immunolocalized. The effects of rSsAK-1 and rSsAK-2 on rabbit PBMC proliferation, apoptosis, and migration; Bcl-2, Bcl-xl, Fas, Bax, and NF-κB transcription levels; and IL-2, IFN-γ, IL-4, IL-10, TGF-β1, and IL-17 secretion were detected.ResultsrSsAK-1 and rSsAK-2 were cloned and expressed successfully. Both enzymes were ~57 kDa and contained 17-kDa tagged proteins, and had good catalytic activity and immunoreactivity. The proteins were located in the S. scabiei exoskeleton, chewing mouthparts, legs, stomach, and intestine. SsAK-1 and SsAK-2 were secreted in the pool and epidermis of the skin lesions, which may be involved in S. scabiei–host interaction. rSsAK-1 and rSsAK-2 significantly promoted cell proliferation, induced cell migration, inhibited apoptosis, and increased Bcl-2, Bcl-xl and NF-κB (p65) transcription levels concentration-dependently, and inhibited IL-2, IFN-γ, and IL-10 secretion and promoted IL-4 and IL-17 secretion.ConclusionrSsAK-1 and rSsAK-2 might increase Bcl-2 and Bcl-xl expression by activating the NF-κB signaling pathway to promote cell proliferation and inhibit apoptosis, which induced PBMC survival. By inducing PBMC migration to the infection site, rSsAK-1 and rSsAK-2 shifted the Th1/Th2 balance toward Th2 and changed the Th17/Treg balance, which indicated their immune role in S. scabiei allergic inflammation.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of two recombinant arginine kinases in Sarcoptes Scabiei on host peripheral blood mononuclear cells

et al. 2022

Front. Immunol.

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“…We are just beginning the exploration of the biological roles played by irisin in the regulation of leukocyte migration in inflammation. As increasing evidence has pointed to the important roles played by irisin in metabolic regulation, further investigations of irisin biology may lead to the realization that irisin is at the focal point of metabolic regulation of leukocyte migration, which is an emerging research topic [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Irisin supports integrin-mediated cell adhesion of lymphocytes

Myint

Ito

Appiah

et al. 2021

Biochemistry and Biophysics Reports

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Irisin, a myokine released from skeletal muscle, has recently been found to act as a ligand for the integrins αVβ5, αVβ1, and α5β1 expressed on mesenchymal cells, thereby playing an important role in the metabolic remodeling of the bone, skeletal muscle and adipose tissues. Although the immune-modulatory effects of irisin in chronic inflammation have been documented, its interactions with lymphocytic integrins have yet to be elucidated. Here, we show that irisin supports the cell adhesion of human and mouse lymphocytes. Cell adhesion assays using a panel of inhibitory antibodies to integrins have shown that irisin-mediated lymphocyte adhesion involves multiple integrins including not only α4β1 and α5β1, but also leukocyte-specific αLβ2 and α4β7. Importantly, mouse lymphocytic TK-1 cells that lack the expression of β1 integrins have exhibited αLβ2- and α4β7-mediated cell adhesion to irisin. Irisin has also been demonstrated to bind to purified recombinant integrin αLβ2 and α4β7 proteins. Thus, irisin represents a novel ligand for integrin αLβ2 and α4β7, capable of supporting lymphocyte cell adhesion independently of β1 integrins. These results suggest that irisin may play an important role in regulating lymphocyte adhesion and migration in the inflamed vasculature.

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“…Reduced numbers of all tested lymphocyte subsets in peripheral blood may result from migration to peripheral lymphoid tissues or from cell death. Lymphocytes constantly migrate between peripheral blood and lymphatic tissues, and environmental cues can modulate this process [45,46]. In rats, in physiological conditions, mature lymphocytes migrate predominantly to the spleen or lymph nodes [47,48].…”

Section: Discussionmentioning

confidence: 99%

Intravenous haloperidol and cocaine alter the distribution of T CD4⁺ and B lymphocytes and NKT cells in rats

Jankowski

Ignatowska‐Jankowska

Glac

et al. 2021

Preprint

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Modulation of dopamine transmission evokes strong behavioral effects that can be achieved by psychoactive drugs such as haloperidol or cocaine. Cocaine non-specifically increases dopamine transmission by blocking dopamine active transporter (DAT) and evokes behavioral arousal, while haloperidol is a non-specific dopamine D2 receptor antagonist with sedative effects. Interestingly, dopamine has been found to affect immune cells in addition to its action in the central nervous system. Here we address the possible interactions between haloperidol and cocaine and their effects on both immune cells and behavior in freely moving rats. We use an intravenous model of haloperidol and binge cocaine administration to evaluate the drugs’ impact on the distribution of lymphocyte subsets in both the peripheral blood and the spleen. We assess the drugs’ behavioral effects by measuring locomotor activity. Cocaine evoked a pronounced locomotor response and stereotypic behaviors, both of which were completely blocked after pretreatment with haloperidol. The results suggest that blood lymphopenia which was induced by haloperidol and cocaine (except for NKT cells), is independent of dopaminergic activity and most likely results from the massive secretion of corticosterone. Haloperidol pretreatment prevented the cocaine-induced decrease in NKT cell numbers. On the other hand, the increased systemic dopaminergic activity after cocaine administration is a significant factor in retaining T CD4+ and B lymphocytes in the spleen.Abstract Figure

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Metabolic regulation of T lymphocyte motility and migration

Cited by 14 publications

References 110 publications

Immunomodulatory effects of two recombinant arginine kinases in Sarcoptes Scabiei on host peripheral blood mononuclear cells

Immunomodulatory effects of two recombinant arginine kinases in Sarcoptes Scabiei on host peripheral blood mononuclear cells

Irisin supports integrin-mediated cell adhesion of lymphocytes

Intravenous haloperidol and cocaine alter the distribution of T CD4⁺ and B lymphocytes and NKT cells in rats

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Metabolic regulation of T lymphocyte motility and migration

Cited by 14 publications

References 110 publications

Immunomodulatory effects of two recombinant arginine kinases in Sarcoptes Scabiei on host peripheral blood mononuclear cells

Immunomodulatory effects of two recombinant arginine kinases in Sarcoptes Scabiei on host peripheral blood mononuclear cells

Irisin supports integrin-mediated cell adhesion of lymphocytes

Intravenous haloperidol and cocaine alter the distribution of T CD4+ and B lymphocytes and NKT cells in rats

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Intravenous haloperidol and cocaine alter the distribution of T CD4⁺ and B lymphocytes and NKT cells in rats