Metabolic regulation of pathways of carbohydrate oxidation in potato (<i>Solanum tuberosum</i>) tubers

Centeno, Danilo da Cruz; Oliver, Sandra N.; Nunes‐Nesi, Adriano; Geigenberger, Peter; Machado, Daniel Nolasco; Loureiro, Marcelo Ehlers; Silva, Marco A. P.; Fernie, Alisdair R.

doi:10.1111/j.1399-3054.2008.01109.x

Cited by 12 publications

(5 citation statements)

References 77 publications

(96 reference statements)

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“…The increase in pyruvate levels in these lines was accompanied by a stimulation of respiration and a strong decrease in internal oxygen concentration in growing tubers (Bologa et al, 2003). Interestingly, both COX-and AOX-dependent pathways of respiration were up-regulated in the transgenic tubers compared to wild type (Centeno et al, 2008).…”

Section: Control Of the Internal Oxygen Concentration By The Level Ofmentioning

confidence: 83%

Regulation of Respiration and Fermentation to Control the Plant Internal Oxygen Concentration

Zabalza

Dongen

Froehlich³

et al. 2008

Plant Physiol.

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Plant internal oxygen concentrations can drop well below ambient even when the plant grows under optimal conditions. Using pea (Pisum sativum) roots, we show how amenable respiration adapts to hypoxia to save oxygen when the oxygen availability decreases. The data cannot simply be explained by oxygen being limiting as substrate but indicate the existence of a regulatory mechanism, because the oxygen concentration at which the adaptive response is initiated is independent of the actual respiratory rate. Two phases can be discerned during the adaptive reaction: an initial linear decline of respiration is followed by a nonlinear inhibition in which the respiratory rate decreased progressively faster upon decreasing oxygen availability. In contrast to the cytochrome c pathway, the inhibition of the alternative oxidase pathway shows only the linear component of the adaptive response. Feeding pyruvate to the roots led to an increase of the oxygen consumption rate, which ultimately led to anoxia. The importance of balancing the in vivo pyruvate availability in the tissue was further investigated. Using various alcohol dehydrogenase knockout lines of Arabidopsis (Arabidopsis thaliana), it was shown that even under aerobic conditions, alcohol fermentation plays an important role in the control of the level of pyruvate in the tissue. Interestingly, alcohol fermentation appeared to be primarily induced by a drop in the energy status of the tissue rather than by a low oxygen concentration, indicating that sensing the energy status is an important component of optimizing plant metabolism to changes in the oxygen availability.

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Section: Control Of the Internal Oxygen Concentration By The Level Ofmentioning

confidence: 83%

Regulation of Respiration and Fermentation to Control the Plant Internal Oxygen Concentration

Zabalza

Dongen

Froehlich³

et al. 2008

Plant Physiol.

Self Cite

243

268

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“…The end‐products of glycolysis (such as pyruvate) in plants are incorporated into the TCA cycle. This process produces a large amount of energy and is the most effective way to obtain energy from the oxidation of sugar and other substances (Centeno et al, ; Li et al, ).…”

Section: Discussionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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Alkali soil is a major abiotic constraint that limits plant distribution and yield in the northeast of China. Puccinellia tenuiflora is considered the most promising grass species for salt‐alkali grassland restoration. However, there is little information on the molecular mechanisms underlying how arbuscular mycorrhizal fungi (AMF) enhances P. tenuiflora stress responses in alkali‐degraded soil. In this study, AMF colonization, growth, photosynthetic pigments, and inorganic ion contents were measured. Isobaric tags for relative and absolute quantification‐based quantitative proteomic technology were employed to identify the differentially abundant proteins in P. tenuiflora seedlings with or without AMF under alkalinity stress. The results showed that AMF colonization increased seedling biomass, photosynthetic pigment contents, and K+/Na+ ratio under alkalinity stress. Moreover, a total of 598 proteins were significantly differentially regulated in P. tenuiflora seedlings after AMF inoculation under alkalinity stress compared with under alkalinity stress alone. The results showed that AMF inoculation significantly improved protein synthesis, reactive oxygen species scavenging, and nitrogen metabolism to promote the biosynthesis of osmotic substances in response to alkalinity stress. In addition, P. tenuiflora seedlings produced more energy to compensate for the energy loss caused by alkalinity stress after AMF inoculation. In conclusion, these findings provide new insights into the physiological mechanisms of the response to alkali‐degraded soil in P. tenuiflora seedlings with AMF and also clarify the role of AMF in the molecular regulation network of P. tenuiflora under alkalinity stress.

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“…A concerted regulation of glycolysis at the protein (Umeda and Uchimiya 1994; Centeno et al 2008) and gene (Wasaki et al 2003) level is believed to maintain a constant metabolic flux through the glycolytic pathway. We hypothesized that a metabolite-mediated regulation of gene expression is the basis of the observed down-regulation of glycolysis in the non-isoprene emitting plants.…”

Section: Discussionmentioning

confidence: 99%

RNAi-mediated suppression of isoprene emission in poplar transiently impacts phenolic metabolism under high temperature and high light intensities: a transcriptomic and metabolomic analysis

et al. 2010

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In plants, isoprene plays a dual role: (a) as thermo-protective agent proposed to prevent degradation of enzymes/membrane structures involved in photosynthesis, and (b) as reactive molecule reducing abiotic oxidative stress. The present work addresses the question whether suppression of isoprene emission interferes with genome wide transcription rates and metabolite fluxes in grey poplar (Populusxcanescens) throughout the growing season. Gene expression and metabolite profiles of isoprene emitting wild type plants and RNAi-mediated non-isoprene emitting poplars were compared by using poplar Affymetrix microarrays and non-targeted FT-ICR-MS (Fourier transform ion cyclotron resonance mass spectrometry). We observed a transcriptional down-regulation of genes encoding enzymes of phenylpropanoid regulatory and biosynthetic pathways, as well as distinct metabolic down-regulation of condensed tannins and anthocyanins, in non-isoprene emitting genotypes during July, when high temperature and light intensities possibly caused transient drought stress, as indicated by stomatal closure. Under these conditions leaves of non-isoprene emitting plants accumulated hydrogen peroxide (H2O2), a signaling molecule in stress response and negative regulator of anthocyanin biosynthesis. The absence of isoprene emission under high temperature and light stress resulted transiently in a new chemo(pheno)type with suppressed production of phenolic compounds. This may compromise inducible defenses and may render non-isoprene emitting poplars more susceptible to environmental stress.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-010-9654-z) contains supplementary material, which is available to authorized users.

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Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers

Cited by 12 publications

References 77 publications

Regulation of Respiration and Fermentation to Control the Plant Internal Oxygen Concentration

Regulation of Respiration and Fermentation to Control the Plant Internal Oxygen Concentration

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

RNAi-mediated suppression of isoprene emission in poplar transiently impacts phenolic metabolism under high temperature and high light intensities: a transcriptomic and metabolomic analysis

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