Metabolic Regulation of Floral Scent in<i>Petunia axillaris</i>Lines: Biosynthetic Relationship between Dihydroconiferyl Acetate and<i>iso</i>-Eugenol

Kondo, Masatoshi; Oyama-Okubo, Naomi; Sagae, Masanori; Ando, Toshio; Marchesi, Eduardo; Nakayama, Masayoshi

doi:10.1271/bbb.60507

Cited by 12 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Species have been identified with as little as one compound emitted by Nicotiana africana to 35 in N. bonariensis during the night (Raguso et al, 2006). Other species such as Petunia (Kondo et al, 2007) have a range of scent components between 10 and 21, similar to the one found in the current study. The diversity of compounds produced in Antirrhinum is large but 500 VOCs have been described in studies in roses demonstrating the possibility of being a highly complex trait (Knudsen et al, 2006).…”

Section: Discussionsupporting

confidence: 86%

Phenotypic Space and Variation of Floral Scent Profiles during Late Flower Development in Antirrhinum

et al. 2016

View full text Add to dashboard Cite

The genus Antirrhinum comprises about 28 species with a center of origin in the Iberian Peninsula. They show an important diversity of growing niches. We have performed a comprehensive analysis of scent profiles in eight wild species, Antirrhinum linkianum, A. tortuosum, A. cirrigherum, A. latifolium, A. meonanthum, A. braun-blanquetii, A. barrelieri, and A. graniticum. We used also two laboratory inbred lines A. majus, 165E and Sippe50. We identified 63 volatile organic compounds (VOCs) belonging to phenylpropanoids, benzenoids, mono- and sesquiterpenes, nitrogen-containing compounds, and aliphatic alcohols previously described in plants. Twenty-four VOCs were produced at levels higher than 2% of total VOC emission, while other VOCs were emitted in trace amounts. The absolute scent emission varied during flower maturation and species. The lowest emitting was A. meonanthum while A. tortuosum had the largest emissions. Species were clustered according to their scent profiles and the resulting dendrogram matched the current species phylogeny. However, two accessions, A. majus Sippe 50 and A. braun-blanquetii, showed development-specific changes in their VOC composition, suggesting a precise control and fine tuning of scent profiles. Cluster analysis of the different scent components failed to identify a specific synthesis pathway, indicating a key role of scent profiles as blends. There is considerable degree of chemodiversity in scent profiles in Antirrhinum. The specific developmental stage plays an important role in scent quantitative emissions. The relative robustness of the bouquets could be an adaptation to local pollinators.

show abstract

Section: Discussionsupporting

confidence: 86%

Phenotypic Space and Variation of Floral Scent Profiles during Late Flower Development in Antirrhinum

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The total amount of endogenous scent compounds decreased as the temperature increased. As the endogenous amount of the floral scent would probably be regulated by biosynthesis and metabolic conversion in P. axillaris flowers, 9) the present results indicate that increasing temperature reduced the biosynthetic activity and/or promoted metabolic conversion activity.…”

Section: Discussionmentioning

confidence: 54%

Effect of Temperature on the Floral Scent Emission and Endogenous Volatile Profile ofPetunia axillaris

Sagae

Oyama-Okubo

Ando

et al. 2008

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

“…Kondo et al. (2007) also examined scent biosynthesis and emission in P. axillaris , confirming that flowers of several P. axillaris parodii accessions as well as some P. axillaris axillaris accessions emitted little or no isoeugenol and eugenol.…”

Section: Introductionmentioning

confidence: 68%

The lack of floral synthesis and emission of isoeugenol in Petunia axillaris subsp. parodii is due to a mutation in the isoeugenol synthase gene

et al. 2009

View full text Add to dashboard Cite

SUMMARYFloral scent has been extensively investigated in plants of the South American genus Petunia. Flowers of Petunia integrifolia emit mostly benzaldehyde, while flowers of Petunia axillaris subsp. axillaris emit a mixture of volatile benzenoid and phenylpropanoid compounds that include isoeugenol and eugenol. Flowers of the artificial hybrid Petunia hybrida, a cross between P. integrifolia and P. axillaris, emit a similar spectrum of volatiles as P. axillaris subsp. axillaris. However, the flowers of P. axillaris subsp. parodii emit neither isoeugenol nor eugenol but contain high levels of dihydroconiferyl acetate in the petals, the main scentsynthesizing and scent-emitting organs. We recently showed that both isoeugenol and eugenol in P. hybrida are biosynthesized from coniferyl acetate in reactions catalyzed by isoeugenol synthase (PhIGS1) and eugenol synthase (PhEGS1), respectively, via a quinone methide-like intermediate. Here we show that P. axillaris subsp. parodii has a functional EGS gene that is expressed in flowers, but its IGS gene contains a frame-shift mutation that renders it inactive. Despite the presence of active EGS enzyme in P. axillaris subsp. parodii, in the absence of IGS activity the coniferyl acetate substrate is converted by an as yet unknown enzyme to dihydroconiferyl acetate. By contrast, suppressing the expression of PhIGS1 in P. hybrida by RNA interference also leads to a decrease in isoeugenol biosynthesis, but instead of the accumulation of dihydroconiferyl acetate, the flowers synthesize higher levels of eugenol.

show abstract

Metabolic Regulation of Floral Scent inPetunia axillarisLines: Biosynthetic Relationship between Dihydroconiferyl Acetate andiso-Eugenol

Cited by 12 publications

References 10 publications

Phenotypic Space and Variation of Floral Scent Profiles during Late Flower Development in Antirrhinum

Phenotypic Space and Variation of Floral Scent Profiles during Late Flower Development in Antirrhinum

Effect of Temperature on the Floral Scent Emission and Endogenous Volatile Profile ofPetunia axillaris

The lack of floral synthesis and emission of isoeugenol in Petunia axillaris subsp. parodii is due to a mutation in the isoeugenol synthase gene

Contact Info

Product

Resources

About