Metabolic Properties of Cells Isolated From Adult Mouse Liver

Berry, Michael N.

doi:10.1083/jcb.15.1.1

Cited by 52 publications

(28 citation statements)

References 21 publications

(30 reference statements)

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“…protein synthesis , urea-cycle activity (Berry, 1962), nucleic acid synthesis (Jacob Vol. 126 & Bhargava, 1965a,b;Jezyk & Liberti, 1969), gluconeogenesis (Berry & Friend, 1969) and ethanol oxidation (Berry, 1971); (5) retention of tissue organizational specificity (Bhargava & Bhargava, 1968).…”

Section: Studies With Mechanically Prepared Cellsmentioning

confidence: 99%

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Crisp

Pogson

1972

Biochemical Journal

130

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Parenchymal cells contain both glucose 6-phosphatase and fructose 1,6-diphosphatase. The non-parenchymal fraction appears to contain fructose 1,6-diphosphatase, but is devoid of glucose 6-phosphatase. 8. No aldolase A was detectable in the whole liver. Aldolase B occurs in both parenchymal and non-parenchymal tissue. 9. Parenchymal cells prepared by mechanical disruption ofmouse liver with 20 % polyvinyl alcohol exhibit a similar enzyme profile to those prepared enzymically. 10. The methodology involved in the preparation of isolated liver cells is discussed. The importance of the measurement of several parameters as criteria for establishing the viability of parenchymal cells is stressed. 11. The metabolic implications of the results in the present study are discussed.

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Section: Studies With Mechanically Prepared Cellsmentioning

confidence: 99%

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Crisp

Pogson

1972

Biochemical Journal

130

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“…X 6000. medium to the interior of the cells so that intracellular structures would be directly exposed to the constituents of the medium. This may account for the similarities in metabolic behaviour between isolated liver cells and isolated mitochondria (5).…”

Section: Changes In the Cell Membranementioning

confidence: 99%

“…The finding that the plasma membranes of isolated liver cells are partially disrupted provides an explanation for the leakage of enzymes (5,12,13) and other soluble constituents (4) from the cells. The loss of permeability barriers at the cell surface would also allow access of the suspending isolated cells and of perfused but undispersed liver, were not swollen but contained numerous clear areas which appeared to lie within the mitochondrial cristae.…”

Section: Changes In the Cell Membranementioning

confidence: 99%

“…The medium used for perfusion and dispersion of the liver comprised 0.40 M sucrose, 0.02 M tris HC1 buffer, pH 7.3. A portion of the cells was washed once and resuspended in this medium, and the remainder was washed and resuspended in either 0.20M sucrose medium or 0.06 M sucrose, 0.08 U KC1 medium (5). All washing procedures were carried out at 0-4°C using ice cold media.…”

Section: Methodsmentioning

confidence: 99%

“…Isolated cells from adult mouse liver were prepared as described previously (5). The medium used for perfusion and dispersion of the liver comprised 0.40 M sucrose, 0.02 M tris HC1 buffer, pH 7.3.…”

Section: Methodsmentioning

confidence: 99%

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Fine Structure of Cells Isolated From Adult Mouse Liver

Berry

Simpson

1962

The Journal of Cell Biology

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Suspensions of isolated cells in various media were prepared from mouse liver which had been perfused via the portal vein with a buffered medium containing 0.40 M sucrose, and the cells were fixed with osmium tetroxide. Their fine structure was compared with that of cells from perfused and unperfnscd intact liver. Perfusion brought about some separation of the cells with little or no damage to cell membranes. When cells were dispersed in 0.40 u sucrose medium the plasma membranes partially broke down, and this disintegration was increased by transfer of the cells to media of lower osmolarity. This is presumed to account for the loss of permeability barriers which occurs in isolated liver cells. The mitochondria in cells of perfused liver and in isolated cells remained elongated, but the layers of many mitochondrial cristae became separated by clear spaces. When cells were transferred to a medium containing 0.20 M sucrose, the mitochondria swelled and became spherical, often with displacement of the swollen cristae to the periphery. In a medium containing 0.06 M sucrose and 0.08 M potassium chloride the outer chamber of many mitochondria became swollen with displacement of the mitochondrial body to one side to give a crescent-shaped appearance. These changes in mitochondrial morphology are discussed in relation to the metabolic activity of isolated liver cells.

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